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Motor-like DNA motion due to an ATP-hydrolyzing protein under nanoconfinement
We report that long double-stranded DNA confined to quasi-1D nanochannels undergoes superdiffusive motion under the action of the enzyme T4 DNA ligase in the presence of necessary co-factors. Inside the confined environment of the nanochannel, double-stranded DNA molecules stretch out due to self-av...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6030079/ https://www.ncbi.nlm.nih.gov/pubmed/29968756 http://dx.doi.org/10.1038/s41598-018-28278-0 |
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author | Roushan, Maedeh Azad, Zubair Movahed, Saeid Ray, Paul D. Livshits, Gideon I. Lim, Shuang Fang Weninger, Keith R. Riehn, Robert |
author_facet | Roushan, Maedeh Azad, Zubair Movahed, Saeid Ray, Paul D. Livshits, Gideon I. Lim, Shuang Fang Weninger, Keith R. Riehn, Robert |
author_sort | Roushan, Maedeh |
collection | PubMed |
description | We report that long double-stranded DNA confined to quasi-1D nanochannels undergoes superdiffusive motion under the action of the enzyme T4 DNA ligase in the presence of necessary co-factors. Inside the confined environment of the nanochannel, double-stranded DNA molecules stretch out due to self-avoiding interactions. In absence of a catalytically active enzyme, we see classical diffusion of the center of mass. However, cooperative interactions of proteins with the DNA can lead to directed motion of DNA molecules inside the nanochannel. Here we show directed motion in this configuration for three different proteins (T4 DNA ligase, MutS, E. coli DNA ligase) in the presence of their energetic co-factors (ATP, NAD(+)). |
format | Online Article Text |
id | pubmed-6030079 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-60300792018-07-11 Motor-like DNA motion due to an ATP-hydrolyzing protein under nanoconfinement Roushan, Maedeh Azad, Zubair Movahed, Saeid Ray, Paul D. Livshits, Gideon I. Lim, Shuang Fang Weninger, Keith R. Riehn, Robert Sci Rep Article We report that long double-stranded DNA confined to quasi-1D nanochannels undergoes superdiffusive motion under the action of the enzyme T4 DNA ligase in the presence of necessary co-factors. Inside the confined environment of the nanochannel, double-stranded DNA molecules stretch out due to self-avoiding interactions. In absence of a catalytically active enzyme, we see classical diffusion of the center of mass. However, cooperative interactions of proteins with the DNA can lead to directed motion of DNA molecules inside the nanochannel. Here we show directed motion in this configuration for three different proteins (T4 DNA ligase, MutS, E. coli DNA ligase) in the presence of their energetic co-factors (ATP, NAD(+)). Nature Publishing Group UK 2018-07-03 /pmc/articles/PMC6030079/ /pubmed/29968756 http://dx.doi.org/10.1038/s41598-018-28278-0 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Roushan, Maedeh Azad, Zubair Movahed, Saeid Ray, Paul D. Livshits, Gideon I. Lim, Shuang Fang Weninger, Keith R. Riehn, Robert Motor-like DNA motion due to an ATP-hydrolyzing protein under nanoconfinement |
title | Motor-like DNA motion due to an ATP-hydrolyzing protein under nanoconfinement |
title_full | Motor-like DNA motion due to an ATP-hydrolyzing protein under nanoconfinement |
title_fullStr | Motor-like DNA motion due to an ATP-hydrolyzing protein under nanoconfinement |
title_full_unstemmed | Motor-like DNA motion due to an ATP-hydrolyzing protein under nanoconfinement |
title_short | Motor-like DNA motion due to an ATP-hydrolyzing protein under nanoconfinement |
title_sort | motor-like dna motion due to an atp-hydrolyzing protein under nanoconfinement |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6030079/ https://www.ncbi.nlm.nih.gov/pubmed/29968756 http://dx.doi.org/10.1038/s41598-018-28278-0 |
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