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SSPIM: a beam shaping toolbox for structured selective plane illumination microscopy

Selective plane illumination microscopy (SPIM) represents a preferred method in dynamic tissue imaging, because it combines high spatiotemporal resolution with low phototoxicity. The OpenSPIM system was developed to provide an accessible and flexible microscope set-up for non-specialist users. Here,...

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Autores principales: Aakhte, Mostafa, Akhlaghi, Ehsan A., Müller, H.-Arno J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6030192/
https://www.ncbi.nlm.nih.gov/pubmed/29968787
http://dx.doi.org/10.1038/s41598-018-28389-8
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author Aakhte, Mostafa
Akhlaghi, Ehsan A.
Müller, H.-Arno J.
author_facet Aakhte, Mostafa
Akhlaghi, Ehsan A.
Müller, H.-Arno J.
author_sort Aakhte, Mostafa
collection PubMed
description Selective plane illumination microscopy (SPIM) represents a preferred method in dynamic tissue imaging, because it combines high spatiotemporal resolution with low phototoxicity. The OpenSPIM system was developed to provide an accessible and flexible microscope set-up for non-specialist users. Here, we report Structured SPIM (SSPIM), which offers an open-source, user-friendly and compact toolbox for beam shaping to be applied within the OpenSPIM platform. SSPIM is able to generate digital patterns for a wide range of illumination beams including static and spherical Gaussian beams, Bessel beams and Airy beams by controlling the pattern of a Spatial Light Modulator (SLM). In addition, SSPIM can produce patterns for structured illumination including incoherent and coherent array beams and tiling for all types of the supported beams. We describe the workflow of the toolbox and demonstrate its application by comparing experimental data with simulation results for a wide range of illumination beams. Finally, the capability of SSPIM is investigated by 3D imaging of Drosophila embryos using scanned Gaussian, Bessel and array beams. SSPIM provides an accessible toolbox to generate and optimize the desired beam patterns and helps adapting the OpenSPIM system towards a wider range of biological samples.
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spelling pubmed-60301922018-07-11 SSPIM: a beam shaping toolbox for structured selective plane illumination microscopy Aakhte, Mostafa Akhlaghi, Ehsan A. Müller, H.-Arno J. Sci Rep Article Selective plane illumination microscopy (SPIM) represents a preferred method in dynamic tissue imaging, because it combines high spatiotemporal resolution with low phototoxicity. The OpenSPIM system was developed to provide an accessible and flexible microscope set-up for non-specialist users. Here, we report Structured SPIM (SSPIM), which offers an open-source, user-friendly and compact toolbox for beam shaping to be applied within the OpenSPIM platform. SSPIM is able to generate digital patterns for a wide range of illumination beams including static and spherical Gaussian beams, Bessel beams and Airy beams by controlling the pattern of a Spatial Light Modulator (SLM). In addition, SSPIM can produce patterns for structured illumination including incoherent and coherent array beams and tiling for all types of the supported beams. We describe the workflow of the toolbox and demonstrate its application by comparing experimental data with simulation results for a wide range of illumination beams. Finally, the capability of SSPIM is investigated by 3D imaging of Drosophila embryos using scanned Gaussian, Bessel and array beams. SSPIM provides an accessible toolbox to generate and optimize the desired beam patterns and helps adapting the OpenSPIM system towards a wider range of biological samples. Nature Publishing Group UK 2018-07-03 /pmc/articles/PMC6030192/ /pubmed/29968787 http://dx.doi.org/10.1038/s41598-018-28389-8 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Aakhte, Mostafa
Akhlaghi, Ehsan A.
Müller, H.-Arno J.
SSPIM: a beam shaping toolbox for structured selective plane illumination microscopy
title SSPIM: a beam shaping toolbox for structured selective plane illumination microscopy
title_full SSPIM: a beam shaping toolbox for structured selective plane illumination microscopy
title_fullStr SSPIM: a beam shaping toolbox for structured selective plane illumination microscopy
title_full_unstemmed SSPIM: a beam shaping toolbox for structured selective plane illumination microscopy
title_short SSPIM: a beam shaping toolbox for structured selective plane illumination microscopy
title_sort sspim: a beam shaping toolbox for structured selective plane illumination microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6030192/
https://www.ncbi.nlm.nih.gov/pubmed/29968787
http://dx.doi.org/10.1038/s41598-018-28389-8
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