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Differentiation of human male germ cells from Wharton's jelly-derived mesenchymal stem cells
OBJECTIVE: Recapitulation of the spermatogenesis process in vitro is a tool for studying the biology of germ cells, and may lead to promising therapeutic strategies in the future. In this study, we attempted to transdifferentiate Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) into mal...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society for Reproductive Medicine
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6030615/ https://www.ncbi.nlm.nih.gov/pubmed/29984207 http://dx.doi.org/10.5653/cerm.2018.45.2.75 |
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author | Dissanayake, DMAB Patel, H Wijesinghe, PS |
author_facet | Dissanayake, DMAB Patel, H Wijesinghe, PS |
author_sort | Dissanayake, DMAB |
collection | PubMed |
description | OBJECTIVE: Recapitulation of the spermatogenesis process in vitro is a tool for studying the biology of germ cells, and may lead to promising therapeutic strategies in the future. In this study, we attempted to transdifferentiate Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) into male germ cells using all-trans retinoic acid and Sertoli cell-conditioned medium. METHODS: Human WJ-MSCs were propagated by the explant culture method, and cells at the second passage were induced with differentiation medium containing all-trans retinoic acid for 2 weeks. Putative germ cells were cultured with Sertoli cell-conditioned medium at 36℃ for 3 more weeks. RESULTS: The gene expression profile was consistent with the stage-specific development of germ cells. The expression of Oct4 and Plzf (early germ cell markers) was diminished, while Stra8 (a premeiotic marker), Scp3 (a meiotic marker), and Acr and Prm1 (postmeiotic markers) were upregulated during the induction period. In morphological studies, approximately 5% of the cells were secondary spermatocytes that had completed two stages of acrosome formation (the Golgi phase and the cap phase). A few spermatid-like cells that had undergone the initial stage of tail formation were also noted. CONCLUSION: Human WJ-MSCs can be transdifferentiated into more advanced stages of germ cells by a simple two-step induction protocol using retinoic acid and Sertoli cell-conditioned medium. |
format | Online Article Text |
id | pubmed-6030615 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Korean Society for Reproductive Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-60306152018-07-07 Differentiation of human male germ cells from Wharton's jelly-derived mesenchymal stem cells Dissanayake, DMAB Patel, H Wijesinghe, PS Clin Exp Reprod Med Original Article OBJECTIVE: Recapitulation of the spermatogenesis process in vitro is a tool for studying the biology of germ cells, and may lead to promising therapeutic strategies in the future. In this study, we attempted to transdifferentiate Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) into male germ cells using all-trans retinoic acid and Sertoli cell-conditioned medium. METHODS: Human WJ-MSCs were propagated by the explant culture method, and cells at the second passage were induced with differentiation medium containing all-trans retinoic acid for 2 weeks. Putative germ cells were cultured with Sertoli cell-conditioned medium at 36℃ for 3 more weeks. RESULTS: The gene expression profile was consistent with the stage-specific development of germ cells. The expression of Oct4 and Plzf (early germ cell markers) was diminished, while Stra8 (a premeiotic marker), Scp3 (a meiotic marker), and Acr and Prm1 (postmeiotic markers) were upregulated during the induction period. In morphological studies, approximately 5% of the cells were secondary spermatocytes that had completed two stages of acrosome formation (the Golgi phase and the cap phase). A few spermatid-like cells that had undergone the initial stage of tail formation were also noted. CONCLUSION: Human WJ-MSCs can be transdifferentiated into more advanced stages of germ cells by a simple two-step induction protocol using retinoic acid and Sertoli cell-conditioned medium. The Korean Society for Reproductive Medicine 2018-06 2018-06-29 /pmc/articles/PMC6030615/ /pubmed/29984207 http://dx.doi.org/10.5653/cerm.2018.45.2.75 Text en Copyright © 2018. The Korean Society for Reproductive Medicine http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Dissanayake, DMAB Patel, H Wijesinghe, PS Differentiation of human male germ cells from Wharton's jelly-derived mesenchymal stem cells |
title | Differentiation of human male germ cells from Wharton's jelly-derived mesenchymal stem cells |
title_full | Differentiation of human male germ cells from Wharton's jelly-derived mesenchymal stem cells |
title_fullStr | Differentiation of human male germ cells from Wharton's jelly-derived mesenchymal stem cells |
title_full_unstemmed | Differentiation of human male germ cells from Wharton's jelly-derived mesenchymal stem cells |
title_short | Differentiation of human male germ cells from Wharton's jelly-derived mesenchymal stem cells |
title_sort | differentiation of human male germ cells from wharton's jelly-derived mesenchymal stem cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6030615/ https://www.ncbi.nlm.nih.gov/pubmed/29984207 http://dx.doi.org/10.5653/cerm.2018.45.2.75 |
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