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CRISPOR: intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens

CRISPOR.org is a web tool for genome editing experiments with the CRISPR–Cas9 system. It finds guide RNAs in an input sequence and ranks them according to different scores that evaluate potential off-targets in the genome of interest and predict on-target activity. The list of genomes is continuousl...

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Detalles Bibliográficos
Autores principales: Concordet, Jean-Paul, Haeussler, Maximilian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6030908/
https://www.ncbi.nlm.nih.gov/pubmed/29762716
http://dx.doi.org/10.1093/nar/gky354
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author Concordet, Jean-Paul
Haeussler, Maximilian
author_facet Concordet, Jean-Paul
Haeussler, Maximilian
author_sort Concordet, Jean-Paul
collection PubMed
description CRISPOR.org is a web tool for genome editing experiments with the CRISPR–Cas9 system. It finds guide RNAs in an input sequence and ranks them according to different scores that evaluate potential off-targets in the genome of interest and predict on-target activity. The list of genomes is continuously expanded, with more 150 genomes added in the last two years. CRISPOR tries to provide a comprehensive solution from selection, cloning and expression of guide RNA as well as providing primers needed for testing guide activity and potential off-targets. Recent developments include batch design for genome-wide CRISPR and saturation screens, creating custom oligonucleotides for guide cloning and the design of next generation sequencing primers to test for off-target mutations. CRISPOR is available from http://crispor.org, including the full source code of the website and a stand-alone, command-line version.
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spelling pubmed-60309082018-07-10 CRISPOR: intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens Concordet, Jean-Paul Haeussler, Maximilian Nucleic Acids Res Web Server Issue CRISPOR.org is a web tool for genome editing experiments with the CRISPR–Cas9 system. It finds guide RNAs in an input sequence and ranks them according to different scores that evaluate potential off-targets in the genome of interest and predict on-target activity. The list of genomes is continuously expanded, with more 150 genomes added in the last two years. CRISPOR tries to provide a comprehensive solution from selection, cloning and expression of guide RNA as well as providing primers needed for testing guide activity and potential off-targets. Recent developments include batch design for genome-wide CRISPR and saturation screens, creating custom oligonucleotides for guide cloning and the design of next generation sequencing primers to test for off-target mutations. CRISPOR is available from http://crispor.org, including the full source code of the website and a stand-alone, command-line version. Oxford University Press 2018-07-02 2018-05-14 /pmc/articles/PMC6030908/ /pubmed/29762716 http://dx.doi.org/10.1093/nar/gky354 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Web Server Issue
Concordet, Jean-Paul
Haeussler, Maximilian
CRISPOR: intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens
title CRISPOR: intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens
title_full CRISPOR: intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens
title_fullStr CRISPOR: intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens
title_full_unstemmed CRISPOR: intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens
title_short CRISPOR: intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens
title_sort crispor: intuitive guide selection for crispr/cas9 genome editing experiments and screens
topic Web Server Issue
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6030908/
https://www.ncbi.nlm.nih.gov/pubmed/29762716
http://dx.doi.org/10.1093/nar/gky354
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