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Assessment of Capacity to Capture DNA Aerosols by Clean Filters for Molecular Biology Experiments
Experimental contamination by exogenous DNA is a major issue in molecular biological studies for data quality and its management. We herein assessed DNA aerosols for the risk of contamination and tested the capacity of clean air filters to trap and remove DNA aerosols. DNA aerosols were generated by...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI)
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6031387/ https://www.ncbi.nlm.nih.gov/pubmed/29910221 http://dx.doi.org/10.1264/jsme2.ME18012 |
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author | Morono, Yuki Hoshino, Tatsuhiko Terada, Takeshi Suzuki, Taketo Sato, Takahiro Yuasa, Hisashi Kubota, Yuji Inagaki, Fumio |
author_facet | Morono, Yuki Hoshino, Tatsuhiko Terada, Takeshi Suzuki, Taketo Sato, Takahiro Yuasa, Hisashi Kubota, Yuji Inagaki, Fumio |
author_sort | Morono, Yuki |
collection | PubMed |
description | Experimental contamination by exogenous DNA is a major issue in molecular biological studies for data quality and its management. We herein assessed DNA aerosols for the risk of contamination and tested the capacity of clean air filters to trap and remove DNA aerosols. DNA aerosols were generated by atomizing a DNA solution and introduced into a laminar flow clean air unit. Capture and detection performed upstream and downstream of the clean air unit showed that a significant fraction (>99.96%) of introduced molecules was trapped and removed by the filter. Although DNA aerosols appear to be an avoidable source of exogenous contamination, a clearer understanding and careful experimental procedures are needed in order to perform contamination-free, high-quality molecular biology experiments. |
format | Online Article Text |
id | pubmed-6031387 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-60313872018-07-06 Assessment of Capacity to Capture DNA Aerosols by Clean Filters for Molecular Biology Experiments Morono, Yuki Hoshino, Tatsuhiko Terada, Takeshi Suzuki, Taketo Sato, Takahiro Yuasa, Hisashi Kubota, Yuji Inagaki, Fumio Microbes Environ Articles Experimental contamination by exogenous DNA is a major issue in molecular biological studies for data quality and its management. We herein assessed DNA aerosols for the risk of contamination and tested the capacity of clean air filters to trap and remove DNA aerosols. DNA aerosols were generated by atomizing a DNA solution and introduced into a laminar flow clean air unit. Capture and detection performed upstream and downstream of the clean air unit showed that a significant fraction (>99.96%) of introduced molecules was trapped and removed by the filter. Although DNA aerosols appear to be an avoidable source of exogenous contamination, a clearer understanding and careful experimental procedures are needed in order to perform contamination-free, high-quality molecular biology experiments. the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2018-06 2018-06-16 /pmc/articles/PMC6031387/ /pubmed/29910221 http://dx.doi.org/10.1264/jsme2.ME18012 Text en Copyright © 2018 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Morono, Yuki Hoshino, Tatsuhiko Terada, Takeshi Suzuki, Taketo Sato, Takahiro Yuasa, Hisashi Kubota, Yuji Inagaki, Fumio Assessment of Capacity to Capture DNA Aerosols by Clean Filters for Molecular Biology Experiments |
title | Assessment of Capacity to Capture DNA Aerosols by Clean Filters for Molecular Biology Experiments |
title_full | Assessment of Capacity to Capture DNA Aerosols by Clean Filters for Molecular Biology Experiments |
title_fullStr | Assessment of Capacity to Capture DNA Aerosols by Clean Filters for Molecular Biology Experiments |
title_full_unstemmed | Assessment of Capacity to Capture DNA Aerosols by Clean Filters for Molecular Biology Experiments |
title_short | Assessment of Capacity to Capture DNA Aerosols by Clean Filters for Molecular Biology Experiments |
title_sort | assessment of capacity to capture dna aerosols by clean filters for molecular biology experiments |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6031387/ https://www.ncbi.nlm.nih.gov/pubmed/29910221 http://dx.doi.org/10.1264/jsme2.ME18012 |
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