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Assessment of Capacity to Capture DNA Aerosols by Clean Filters for Molecular Biology Experiments

Experimental contamination by exogenous DNA is a major issue in molecular biological studies for data quality and its management. We herein assessed DNA aerosols for the risk of contamination and tested the capacity of clean air filters to trap and remove DNA aerosols. DNA aerosols were generated by...

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Detalles Bibliográficos
Autores principales: Morono, Yuki, Hoshino, Tatsuhiko, Terada, Takeshi, Suzuki, Taketo, Sato, Takahiro, Yuasa, Hisashi, Kubota, Yuji, Inagaki, Fumio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6031387/
https://www.ncbi.nlm.nih.gov/pubmed/29910221
http://dx.doi.org/10.1264/jsme2.ME18012
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author Morono, Yuki
Hoshino, Tatsuhiko
Terada, Takeshi
Suzuki, Taketo
Sato, Takahiro
Yuasa, Hisashi
Kubota, Yuji
Inagaki, Fumio
author_facet Morono, Yuki
Hoshino, Tatsuhiko
Terada, Takeshi
Suzuki, Taketo
Sato, Takahiro
Yuasa, Hisashi
Kubota, Yuji
Inagaki, Fumio
author_sort Morono, Yuki
collection PubMed
description Experimental contamination by exogenous DNA is a major issue in molecular biological studies for data quality and its management. We herein assessed DNA aerosols for the risk of contamination and tested the capacity of clean air filters to trap and remove DNA aerosols. DNA aerosols were generated by atomizing a DNA solution and introduced into a laminar flow clean air unit. Capture and detection performed upstream and downstream of the clean air unit showed that a significant fraction (>99.96%) of introduced molecules was trapped and removed by the filter. Although DNA aerosols appear to be an avoidable source of exogenous contamination, a clearer understanding and careful experimental procedures are needed in order to perform contamination-free, high-quality molecular biology experiments.
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spelling pubmed-60313872018-07-06 Assessment of Capacity to Capture DNA Aerosols by Clean Filters for Molecular Biology Experiments Morono, Yuki Hoshino, Tatsuhiko Terada, Takeshi Suzuki, Taketo Sato, Takahiro Yuasa, Hisashi Kubota, Yuji Inagaki, Fumio Microbes Environ Articles Experimental contamination by exogenous DNA is a major issue in molecular biological studies for data quality and its management. We herein assessed DNA aerosols for the risk of contamination and tested the capacity of clean air filters to trap and remove DNA aerosols. DNA aerosols were generated by atomizing a DNA solution and introduced into a laminar flow clean air unit. Capture and detection performed upstream and downstream of the clean air unit showed that a significant fraction (>99.96%) of introduced molecules was trapped and removed by the filter. Although DNA aerosols appear to be an avoidable source of exogenous contamination, a clearer understanding and careful experimental procedures are needed in order to perform contamination-free, high-quality molecular biology experiments. the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2018-06 2018-06-16 /pmc/articles/PMC6031387/ /pubmed/29910221 http://dx.doi.org/10.1264/jsme2.ME18012 Text en Copyright © 2018 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Morono, Yuki
Hoshino, Tatsuhiko
Terada, Takeshi
Suzuki, Taketo
Sato, Takahiro
Yuasa, Hisashi
Kubota, Yuji
Inagaki, Fumio
Assessment of Capacity to Capture DNA Aerosols by Clean Filters for Molecular Biology Experiments
title Assessment of Capacity to Capture DNA Aerosols by Clean Filters for Molecular Biology Experiments
title_full Assessment of Capacity to Capture DNA Aerosols by Clean Filters for Molecular Biology Experiments
title_fullStr Assessment of Capacity to Capture DNA Aerosols by Clean Filters for Molecular Biology Experiments
title_full_unstemmed Assessment of Capacity to Capture DNA Aerosols by Clean Filters for Molecular Biology Experiments
title_short Assessment of Capacity to Capture DNA Aerosols by Clean Filters for Molecular Biology Experiments
title_sort assessment of capacity to capture dna aerosols by clean filters for molecular biology experiments
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6031387/
https://www.ncbi.nlm.nih.gov/pubmed/29910221
http://dx.doi.org/10.1264/jsme2.ME18012
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