Ureolytic Prokaryotes in Soil: Community Abundance and Diversity

Although the turnover of urea is a crucial process in nitrogen transformation in soil, limited information is currently available on the abundance and diversity of ureolytic prokaryotes. The abundance and diversity of the soil 16S rRNA gene and ureC (encoding a urease catalytic subunit) were examine...

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Detalles Bibliográficos
Autores principales: Oshiki, Mamoru, Araki, Mitsuru, Hirakata, Yuga, Hatamoto, Masashi, Yamaguchi, Takashi, Araki, Nobuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2018
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6031400/
https://www.ncbi.nlm.nih.gov/pubmed/29709896
http://dx.doi.org/10.1264/jsme2.ME17188
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author Oshiki, Mamoru
Araki, Mitsuru
Hirakata, Yuga
Hatamoto, Masashi
Yamaguchi, Takashi
Araki, Nobuo
author_facet Oshiki, Mamoru
Araki, Mitsuru
Hirakata, Yuga
Hatamoto, Masashi
Yamaguchi, Takashi
Araki, Nobuo
author_sort Oshiki, Mamoru
collection PubMed
description Although the turnover of urea is a crucial process in nitrogen transformation in soil, limited information is currently available on the abundance and diversity of ureolytic prokaryotes. The abundance and diversity of the soil 16S rRNA gene and ureC (encoding a urease catalytic subunit) were examined in seven soil types using quantitative PCR and amplicon sequencing with Illumina MiSeq. The amplicon sequencing of ureC revealed that the ureolytic community was composed of phylogenetically varied prokaryotes, and we detected 363 to 1,685 species-level ureC operational taxonomic units (OTUs) per soil sample, whereas 5,984 OTUs were site-specific OTUs found in only one of the seven soil types.
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spelling pubmed-60314002018-07-06 Ureolytic Prokaryotes in Soil: Community Abundance and Diversity Oshiki, Mamoru Araki, Mitsuru Hirakata, Yuga Hatamoto, Masashi Yamaguchi, Takashi Araki, Nobuo Microbes Environ Short Communication Although the turnover of urea is a crucial process in nitrogen transformation in soil, limited information is currently available on the abundance and diversity of ureolytic prokaryotes. The abundance and diversity of the soil 16S rRNA gene and ureC (encoding a urease catalytic subunit) were examined in seven soil types using quantitative PCR and amplicon sequencing with Illumina MiSeq. The amplicon sequencing of ureC revealed that the ureolytic community was composed of phylogenetically varied prokaryotes, and we detected 363 to 1,685 species-level ureC operational taxonomic units (OTUs) per soil sample, whereas 5,984 OTUs were site-specific OTUs found in only one of the seven soil types. the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2018-06 2018-04-28 /pmc/articles/PMC6031400/ /pubmed/29709896 http://dx.doi.org/10.1264/jsme2.ME17188 Text en Copyright © 2018 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Oshiki, Mamoru
Araki, Mitsuru
Hirakata, Yuga
Hatamoto, Masashi
Yamaguchi, Takashi
Araki, Nobuo
Ureolytic Prokaryotes in Soil: Community Abundance and Diversity
title Ureolytic Prokaryotes in Soil: Community Abundance and Diversity
title_full Ureolytic Prokaryotes in Soil: Community Abundance and Diversity
title_fullStr Ureolytic Prokaryotes in Soil: Community Abundance and Diversity
title_full_unstemmed Ureolytic Prokaryotes in Soil: Community Abundance and Diversity
title_short Ureolytic Prokaryotes in Soil: Community Abundance and Diversity
title_sort ureolytic prokaryotes in soil: community abundance and diversity
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6031400/
https://www.ncbi.nlm.nih.gov/pubmed/29709896
http://dx.doi.org/10.1264/jsme2.ME17188
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