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Mass Spectrometry Based Imaging of Labile Glucosides in Plants
Mass spectrometry based imaging is a powerful tool to investigate the spatial distribution of a broad range of metabolites across a variety of sample types. The recent developments in instrumentation and computing capabilities have increased the mass range, sensitivity and resolution and rendered sa...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6031732/ https://www.ncbi.nlm.nih.gov/pubmed/30002667 http://dx.doi.org/10.3389/fpls.2018.00892 |
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author | Bøgeskov Schmidt, Frederik Heskes, Allison M. Thinagaran, Dinaiz Lindberg Møller, Birger Jørgensen, Kirsten Boughton, Berin A. |
author_facet | Bøgeskov Schmidt, Frederik Heskes, Allison M. Thinagaran, Dinaiz Lindberg Møller, Birger Jørgensen, Kirsten Boughton, Berin A. |
author_sort | Bøgeskov Schmidt, Frederik |
collection | PubMed |
description | Mass spectrometry based imaging is a powerful tool to investigate the spatial distribution of a broad range of metabolites across a variety of sample types. The recent developments in instrumentation and computing capabilities have increased the mass range, sensitivity and resolution and rendered sample preparation the limiting step for further improvements. Sample preparation involves sectioning and mounting followed by selection and application of matrix. In plant tissues, labile small molecules and specialized metabolites are subject to degradation upon mechanical disruption of plant tissues. In this study, the benefits of cryo-sectioning, stabilization of fragile tissues and optimal application of the matrix to improve the results from MALDI mass spectrometry imaging (MSI) is investigated with hydroxynitrile glucosides as the main experimental system. Denatured albumin proved an excellent agent for stabilizing fragile tissues such as Lotus japonicus leaves. In stem cross sections of Manihot esculenta, maintaining the samples frozen throughout the sectioning process and preparation of the samples by freeze drying enhanced the obtained signal intensity by twofold to fourfold. Deposition of the matrix by sublimation improved the spatial information obtained compared to spray. The imaging demonstrated that the cyanogenic glucosides (CNglcs) were localized in the vascular tissues in old stems of M. esculenta and in the periderm and vascular tissues of tubers. In MALDI mass spectrometry, the imaged compounds are solely identified by their m/z ratio. L. japonicus MG20 and the mutant cyd1 that is devoid of hydroxynitrile glucosides were used as negative controls to verify the assignment of the observed masses to linamarin, lotaustralin, and linamarin acid. |
format | Online Article Text |
id | pubmed-6031732 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-60317322018-07-12 Mass Spectrometry Based Imaging of Labile Glucosides in Plants Bøgeskov Schmidt, Frederik Heskes, Allison M. Thinagaran, Dinaiz Lindberg Møller, Birger Jørgensen, Kirsten Boughton, Berin A. Front Plant Sci Plant Science Mass spectrometry based imaging is a powerful tool to investigate the spatial distribution of a broad range of metabolites across a variety of sample types. The recent developments in instrumentation and computing capabilities have increased the mass range, sensitivity and resolution and rendered sample preparation the limiting step for further improvements. Sample preparation involves sectioning and mounting followed by selection and application of matrix. In plant tissues, labile small molecules and specialized metabolites are subject to degradation upon mechanical disruption of plant tissues. In this study, the benefits of cryo-sectioning, stabilization of fragile tissues and optimal application of the matrix to improve the results from MALDI mass spectrometry imaging (MSI) is investigated with hydroxynitrile glucosides as the main experimental system. Denatured albumin proved an excellent agent for stabilizing fragile tissues such as Lotus japonicus leaves. In stem cross sections of Manihot esculenta, maintaining the samples frozen throughout the sectioning process and preparation of the samples by freeze drying enhanced the obtained signal intensity by twofold to fourfold. Deposition of the matrix by sublimation improved the spatial information obtained compared to spray. The imaging demonstrated that the cyanogenic glucosides (CNglcs) were localized in the vascular tissues in old stems of M. esculenta and in the periderm and vascular tissues of tubers. In MALDI mass spectrometry, the imaged compounds are solely identified by their m/z ratio. L. japonicus MG20 and the mutant cyd1 that is devoid of hydroxynitrile glucosides were used as negative controls to verify the assignment of the observed masses to linamarin, lotaustralin, and linamarin acid. Frontiers Media S.A. 2018-06-28 /pmc/articles/PMC6031732/ /pubmed/30002667 http://dx.doi.org/10.3389/fpls.2018.00892 Text en Copyright © 2018 Bøgeskov Schmidt, Heskes, Thinagaran, Lindberg Møller, Jørgensen and Boughton http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Bøgeskov Schmidt, Frederik Heskes, Allison M. Thinagaran, Dinaiz Lindberg Møller, Birger Jørgensen, Kirsten Boughton, Berin A. Mass Spectrometry Based Imaging of Labile Glucosides in Plants |
title | Mass Spectrometry Based Imaging of Labile Glucosides in Plants |
title_full | Mass Spectrometry Based Imaging of Labile Glucosides in Plants |
title_fullStr | Mass Spectrometry Based Imaging of Labile Glucosides in Plants |
title_full_unstemmed | Mass Spectrometry Based Imaging of Labile Glucosides in Plants |
title_short | Mass Spectrometry Based Imaging of Labile Glucosides in Plants |
title_sort | mass spectrometry based imaging of labile glucosides in plants |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6031732/ https://www.ncbi.nlm.nih.gov/pubmed/30002667 http://dx.doi.org/10.3389/fpls.2018.00892 |
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