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Inhibitory Effects of Arsenic Trioxide and Thalidomide on Angiogenesis and Vascular Endothelial Growth Factor Expression in Leukemia Cells

Acute myeloid leukemia (AML) is a blood disorder characterized by uncontrolled proliferation of myeloid progenitors and decrease in the apoptosis rate. The vascular endothelial growth factor (VEGF) promotes blood vessel regeneration which might play important roles in development and progression of...

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Autores principales: Kian, Mahnaz Mohammadi, Mohammadi, Saeed, Tavallaei, Mahmoud, Chahardouli, Bahram, Rostami, Saharbano, Zahedpanah, Mahdi, Ghavamzadeh, Ardeshir, Nikbakht, Mohsen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: West Asia Organization for Cancer Prevention 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6031772/
https://www.ncbi.nlm.nih.gov/pubmed/29699374
http://dx.doi.org/10.22034/APJCP.2018.19.4.1127
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author Kian, Mahnaz Mohammadi
Mohammadi, Saeed
Tavallaei, Mahmoud
Chahardouli, Bahram
Rostami, Saharbano
Zahedpanah, Mahdi
Ghavamzadeh, Ardeshir
Nikbakht, Mohsen
author_facet Kian, Mahnaz Mohammadi
Mohammadi, Saeed
Tavallaei, Mahmoud
Chahardouli, Bahram
Rostami, Saharbano
Zahedpanah, Mahdi
Ghavamzadeh, Ardeshir
Nikbakht, Mohsen
author_sort Kian, Mahnaz Mohammadi
collection PubMed
description Acute myeloid leukemia (AML) is a blood disorder characterized by uncontrolled proliferation of myeloid progenitors and decrease in the apoptosis rate. The vascular endothelial growth factor (VEGF) promotes blood vessel regeneration which might play important roles in development and progression of neoplasia. Our previous studies focused on cytotoxicity and anticancer effects of arsenic trioxide (ATO) and thalidomide (THAL) as an anti-VEGF compound in the AML cell model. ATO also affects regulatory genes involved in cell proliferation and apoptosis. The aim of present study was to examine the effects of ATO and THAL alone and in combination on U937 and KG-1 cells, with attention to mRNA expression for VEGF isoforms. Growth inhibitory effects was assessed by MTT assay and apoptosis induction was determined by Annexin/PI staining. mRNA expression levels were evaluated by real-time PCR. Our data indicated that ATO (1.618μM and 1μM in KG-1 and U937 cell lines respectively), THAL (80μM and 60μM) and their combination inhibited proliferation and induced apoptosis in our cell lines. mRNA expression of VEGF (A, B) decreased while C and D isoforms did not show any significant changes. Taken together, according to the obtained results, the VEGF autocrine loop could be a target as a therapeutic strategy for cases of AML.
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spelling pubmed-60317722018-07-11 Inhibitory Effects of Arsenic Trioxide and Thalidomide on Angiogenesis and Vascular Endothelial Growth Factor Expression in Leukemia Cells Kian, Mahnaz Mohammadi Mohammadi, Saeed Tavallaei, Mahmoud Chahardouli, Bahram Rostami, Saharbano Zahedpanah, Mahdi Ghavamzadeh, Ardeshir Nikbakht, Mohsen Asian Pac J Cancer Prev Research Article Acute myeloid leukemia (AML) is a blood disorder characterized by uncontrolled proliferation of myeloid progenitors and decrease in the apoptosis rate. The vascular endothelial growth factor (VEGF) promotes blood vessel regeneration which might play important roles in development and progression of neoplasia. Our previous studies focused on cytotoxicity and anticancer effects of arsenic trioxide (ATO) and thalidomide (THAL) as an anti-VEGF compound in the AML cell model. ATO also affects regulatory genes involved in cell proliferation and apoptosis. The aim of present study was to examine the effects of ATO and THAL alone and in combination on U937 and KG-1 cells, with attention to mRNA expression for VEGF isoforms. Growth inhibitory effects was assessed by MTT assay and apoptosis induction was determined by Annexin/PI staining. mRNA expression levels were evaluated by real-time PCR. Our data indicated that ATO (1.618μM and 1μM in KG-1 and U937 cell lines respectively), THAL (80μM and 60μM) and their combination inhibited proliferation and induced apoptosis in our cell lines. mRNA expression of VEGF (A, B) decreased while C and D isoforms did not show any significant changes. Taken together, according to the obtained results, the VEGF autocrine loop could be a target as a therapeutic strategy for cases of AML. West Asia Organization for Cancer Prevention 2018 /pmc/articles/PMC6031772/ /pubmed/29699374 http://dx.doi.org/10.22034/APJCP.2018.19.4.1127 Text en Copyright: © Asian Pacific Journal of Cancer Prevention http://creativecommons.org/licenses/BY-SA/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
spellingShingle Research Article
Kian, Mahnaz Mohammadi
Mohammadi, Saeed
Tavallaei, Mahmoud
Chahardouli, Bahram
Rostami, Saharbano
Zahedpanah, Mahdi
Ghavamzadeh, Ardeshir
Nikbakht, Mohsen
Inhibitory Effects of Arsenic Trioxide and Thalidomide on Angiogenesis and Vascular Endothelial Growth Factor Expression in Leukemia Cells
title Inhibitory Effects of Arsenic Trioxide and Thalidomide on Angiogenesis and Vascular Endothelial Growth Factor Expression in Leukemia Cells
title_full Inhibitory Effects of Arsenic Trioxide and Thalidomide on Angiogenesis and Vascular Endothelial Growth Factor Expression in Leukemia Cells
title_fullStr Inhibitory Effects of Arsenic Trioxide and Thalidomide on Angiogenesis and Vascular Endothelial Growth Factor Expression in Leukemia Cells
title_full_unstemmed Inhibitory Effects of Arsenic Trioxide and Thalidomide on Angiogenesis and Vascular Endothelial Growth Factor Expression in Leukemia Cells
title_short Inhibitory Effects of Arsenic Trioxide and Thalidomide on Angiogenesis and Vascular Endothelial Growth Factor Expression in Leukemia Cells
title_sort inhibitory effects of arsenic trioxide and thalidomide on angiogenesis and vascular endothelial growth factor expression in leukemia cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6031772/
https://www.ncbi.nlm.nih.gov/pubmed/29699374
http://dx.doi.org/10.22034/APJCP.2018.19.4.1127
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