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Sirt1 Gene Expression and Gastric Epithelial Cells Tumor Stage in Patients with Helicobacter pylori Infection

INTRODUCTION: The World Health Organization has categorized Helicobacter pylori as a carcinogen for gastric cancer, which causes human mortality worldwide. A number of studies have shown that H. pylori affects cell signaling in gastric epithelial cells and changes the expression of some proteins suc...

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Autores principales: Saravle, Saman Mohammadi, Hedayati, Manouchehr Ahmadi, Mohammadi, Ebrahim, Sheikhesmaeili, Farshad, Nikkhou, Bahram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: West Asia Organization for Cancer Prevention 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6031779/
https://www.ncbi.nlm.nih.gov/pubmed/29693338
http://dx.doi.org/10.22034/APJCP.2018.19.4.913
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author Saravle, Saman Mohammadi
Hedayati, Manouchehr Ahmadi
Mohammadi, Ebrahim
Sheikhesmaeili, Farshad
Nikkhou, Bahram
author_facet Saravle, Saman Mohammadi
Hedayati, Manouchehr Ahmadi
Mohammadi, Ebrahim
Sheikhesmaeili, Farshad
Nikkhou, Bahram
author_sort Saravle, Saman Mohammadi
collection PubMed
description INTRODUCTION: The World Health Organization has categorized Helicobacter pylori as a carcinogen for gastric cancer, which causes human mortality worldwide. A number of studies have shown that H. pylori affects cell signaling in gastric epithelial cells and changes the expression of some proteins such as proinflammatory cytokines. Bacterial infections may alter sirt1 and sirt2 genes expression in inflammatory tissues and cancer cells. In this study, sirt1 and sirt2 genes expression in gastric cancers was surveyed with reference to H. pylori status. METHODS: Stomach biopsies were collected from 50 gastric cancer patients, 25 H. pylori-positive and 25 H. pylori-negative as determined by the urea rapid test. Tumor grade was determined by a pathologist. After total RNA extraction from gastric cancer biopsy samples and cDNA synthesis, sirt1 and sirt2 genes expression levels were determined by Real Time PCR and ΔΔCT methods. RESULTS: There was no statistically significant link between H. pylori infection and sirt1 (P<0.899) and sirt2 (P<0.169) genes expression in gastric epithelial cells. However, pathologic findings showed that there is a statistically significant relationship between sirt1 gene expression and the tumor grade (P<0.024). DISCUSSION: A statistically significant association was found between sirt1 gene expression and tumor grade of gastric cancers that could be due to effects on progression of cancer cells infected with H. pylori.
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spelling pubmed-60317792018-07-11 Sirt1 Gene Expression and Gastric Epithelial Cells Tumor Stage in Patients with Helicobacter pylori Infection Saravle, Saman Mohammadi Hedayati, Manouchehr Ahmadi Mohammadi, Ebrahim Sheikhesmaeili, Farshad Nikkhou, Bahram Asian Pac J Cancer Prev Research Article INTRODUCTION: The World Health Organization has categorized Helicobacter pylori as a carcinogen for gastric cancer, which causes human mortality worldwide. A number of studies have shown that H. pylori affects cell signaling in gastric epithelial cells and changes the expression of some proteins such as proinflammatory cytokines. Bacterial infections may alter sirt1 and sirt2 genes expression in inflammatory tissues and cancer cells. In this study, sirt1 and sirt2 genes expression in gastric cancers was surveyed with reference to H. pylori status. METHODS: Stomach biopsies were collected from 50 gastric cancer patients, 25 H. pylori-positive and 25 H. pylori-negative as determined by the urea rapid test. Tumor grade was determined by a pathologist. After total RNA extraction from gastric cancer biopsy samples and cDNA synthesis, sirt1 and sirt2 genes expression levels were determined by Real Time PCR and ΔΔCT methods. RESULTS: There was no statistically significant link between H. pylori infection and sirt1 (P<0.899) and sirt2 (P<0.169) genes expression in gastric epithelial cells. However, pathologic findings showed that there is a statistically significant relationship between sirt1 gene expression and the tumor grade (P<0.024). DISCUSSION: A statistically significant association was found between sirt1 gene expression and tumor grade of gastric cancers that could be due to effects on progression of cancer cells infected with H. pylori. West Asia Organization for Cancer Prevention 2018 /pmc/articles/PMC6031779/ /pubmed/29693338 http://dx.doi.org/10.22034/APJCP.2018.19.4.913 Text en Copyright: © Asian Pacific Journal of Cancer Prevention http://creativecommons.org/licenses/BY-SA/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
spellingShingle Research Article
Saravle, Saman Mohammadi
Hedayati, Manouchehr Ahmadi
Mohammadi, Ebrahim
Sheikhesmaeili, Farshad
Nikkhou, Bahram
Sirt1 Gene Expression and Gastric Epithelial Cells Tumor Stage in Patients with Helicobacter pylori Infection
title Sirt1 Gene Expression and Gastric Epithelial Cells Tumor Stage in Patients with Helicobacter pylori Infection
title_full Sirt1 Gene Expression and Gastric Epithelial Cells Tumor Stage in Patients with Helicobacter pylori Infection
title_fullStr Sirt1 Gene Expression and Gastric Epithelial Cells Tumor Stage in Patients with Helicobacter pylori Infection
title_full_unstemmed Sirt1 Gene Expression and Gastric Epithelial Cells Tumor Stage in Patients with Helicobacter pylori Infection
title_short Sirt1 Gene Expression and Gastric Epithelial Cells Tumor Stage in Patients with Helicobacter pylori Infection
title_sort sirt1 gene expression and gastric epithelial cells tumor stage in patients with helicobacter pylori infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6031779/
https://www.ncbi.nlm.nih.gov/pubmed/29693338
http://dx.doi.org/10.22034/APJCP.2018.19.4.913
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