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Metagenomics of Microbial Communities in Gallbladder Bile from Patients with Gallbladder Cancer or Cholelithiasis

Salmonella typhi and Helicobacter infections have been shown to increase risk of gallbladder cancer (GBC), but findings have been inconsistent. Other bacterial infections may also be associated with GBC. However, information on microbial pathogens in gallbladder bile of GBC patients is scarce. We ai...

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Autores principales: Tsuchiya, Yasuo, Loza, Ernest, Villa-Gomez, Guido, Trujillo, Carlos C, Baez, Sergio, Asai, Takao, Ikoma, Toshikazu, Endoh, Kazuo, Nakamura, Kazutoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: West Asia Organization for Cancer Prevention 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6031792/
https://www.ncbi.nlm.nih.gov/pubmed/29693356
http://dx.doi.org/10.22034/APJCP.2018.19.4.961
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author Tsuchiya, Yasuo
Loza, Ernest
Villa-Gomez, Guido
Trujillo, Carlos C
Baez, Sergio
Asai, Takao
Ikoma, Toshikazu
Endoh, Kazuo
Nakamura, Kazutoshi
author_facet Tsuchiya, Yasuo
Loza, Ernest
Villa-Gomez, Guido
Trujillo, Carlos C
Baez, Sergio
Asai, Takao
Ikoma, Toshikazu
Endoh, Kazuo
Nakamura, Kazutoshi
author_sort Tsuchiya, Yasuo
collection PubMed
description Salmonella typhi and Helicobacter infections have been shown to increase risk of gallbladder cancer (GBC), but findings have been inconsistent. Other bacterial infections may also be associated with GBC. However, information on microbial pathogens in gallbladder bile of GBC patients is scarce. We aimed to investigate the microbial communities in gallbladder bile of patients with GBC and cholelithiasis (CL). Seven GBC patients and 30 CL patients were enrolled in this study. Genomic DNA was extracted from bile and the V3-V4 region of 16S rRNA was amplified. The sequencing results were compared with the 16S database, and the bacteria were identified by homology searches and phylogenetic analysis. DNA was detected in the bile of three GBC (42.9%; Bolivia, 1; Chile, 2) and four CL patients (13.3%; Bolivia, 1; Chile, 3). Of the 37 patients, 30 (81.1%) were negative and unable to analyze. Salmonella typhi and Helicobacter sp. were not detected in bile from any GBC patients. As the predominant species, Fusobacterium nucleatum, Escherichia coli, and Enetrobacter sp. were detected in bile from GBC patients. Those in bile from CL patients were Escherichia coli, Salmonella sp., and Enerococcus gallinarum. Escherichia coli was detected in bile samples from both GBC and CL patients. Whether the bacteria detected in bile from GBC patients would associated with the development of GBC warrant further investigation.
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spelling pubmed-60317922018-07-11 Metagenomics of Microbial Communities in Gallbladder Bile from Patients with Gallbladder Cancer or Cholelithiasis Tsuchiya, Yasuo Loza, Ernest Villa-Gomez, Guido Trujillo, Carlos C Baez, Sergio Asai, Takao Ikoma, Toshikazu Endoh, Kazuo Nakamura, Kazutoshi Asian Pac J Cancer Prev Research Article Salmonella typhi and Helicobacter infections have been shown to increase risk of gallbladder cancer (GBC), but findings have been inconsistent. Other bacterial infections may also be associated with GBC. However, information on microbial pathogens in gallbladder bile of GBC patients is scarce. We aimed to investigate the microbial communities in gallbladder bile of patients with GBC and cholelithiasis (CL). Seven GBC patients and 30 CL patients were enrolled in this study. Genomic DNA was extracted from bile and the V3-V4 region of 16S rRNA was amplified. The sequencing results were compared with the 16S database, and the bacteria were identified by homology searches and phylogenetic analysis. DNA was detected in the bile of three GBC (42.9%; Bolivia, 1; Chile, 2) and four CL patients (13.3%; Bolivia, 1; Chile, 3). Of the 37 patients, 30 (81.1%) were negative and unable to analyze. Salmonella typhi and Helicobacter sp. were not detected in bile from any GBC patients. As the predominant species, Fusobacterium nucleatum, Escherichia coli, and Enetrobacter sp. were detected in bile from GBC patients. Those in bile from CL patients were Escherichia coli, Salmonella sp., and Enerococcus gallinarum. Escherichia coli was detected in bile samples from both GBC and CL patients. Whether the bacteria detected in bile from GBC patients would associated with the development of GBC warrant further investigation. West Asia Organization for Cancer Prevention 2018 /pmc/articles/PMC6031792/ /pubmed/29693356 http://dx.doi.org/10.22034/APJCP.2018.19.4.961 Text en Copyright: © Asian Pacific Journal of Cancer Prevention http://creativecommons.org/licenses/BY-SA/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
spellingShingle Research Article
Tsuchiya, Yasuo
Loza, Ernest
Villa-Gomez, Guido
Trujillo, Carlos C
Baez, Sergio
Asai, Takao
Ikoma, Toshikazu
Endoh, Kazuo
Nakamura, Kazutoshi
Metagenomics of Microbial Communities in Gallbladder Bile from Patients with Gallbladder Cancer or Cholelithiasis
title Metagenomics of Microbial Communities in Gallbladder Bile from Patients with Gallbladder Cancer or Cholelithiasis
title_full Metagenomics of Microbial Communities in Gallbladder Bile from Patients with Gallbladder Cancer or Cholelithiasis
title_fullStr Metagenomics of Microbial Communities in Gallbladder Bile from Patients with Gallbladder Cancer or Cholelithiasis
title_full_unstemmed Metagenomics of Microbial Communities in Gallbladder Bile from Patients with Gallbladder Cancer or Cholelithiasis
title_short Metagenomics of Microbial Communities in Gallbladder Bile from Patients with Gallbladder Cancer or Cholelithiasis
title_sort metagenomics of microbial communities in gallbladder bile from patients with gallbladder cancer or cholelithiasis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6031792/
https://www.ncbi.nlm.nih.gov/pubmed/29693356
http://dx.doi.org/10.22034/APJCP.2018.19.4.961
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