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Ultrafiltration and size exclusion chromatography combined with asymmetrical-flow field-flow fractionation for the isolation and characterisation of extracellular vesicles from urine
Extracellular vesicles (EVs) have a great potential in clinical applications. However, their isolation from different bodily fluids and their characterisation are currently not optimal or standardised. Here, we report the results of examining the performance of ultrafiltration combined with size exc...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6032024/ https://www.ncbi.nlm.nih.gov/pubmed/29988836 http://dx.doi.org/10.1080/20013078.2018.1490143 |
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author | Oeyen, Eline Van Mol, Kurt Baggerman, Geert Willems, Hanny Boonen, Kurt Rolfo, Christian Pauwels, Patrick Jacobs, An Schildermans, Karin Cho, William C Mertens, Inge |
author_facet | Oeyen, Eline Van Mol, Kurt Baggerman, Geert Willems, Hanny Boonen, Kurt Rolfo, Christian Pauwels, Patrick Jacobs, An Schildermans, Karin Cho, William C Mertens, Inge |
author_sort | Oeyen, Eline |
collection | PubMed |
description | Extracellular vesicles (EVs) have a great potential in clinical applications. However, their isolation from different bodily fluids and their characterisation are currently not optimal or standardised. Here, we report the results of examining the performance of ultrafiltration combined with size exclusion chromatography (UF-SEC) to isolate EVs from urine. The results reveal that UF-SEC is an efficient method and provides high purity. Furthermore, we introduce asymmetrical-flow field-flow fractionation coupled with a UV detector and multi-angle light-scattering detector (AF4/UV-MALS) as a characterisation method and compare it with current methods. We demonstrate that AF4/UV-MALS is a straightforward and reproducible method for determining size, amount and purity of isolated urinary EVs. |
format | Online Article Text |
id | pubmed-6032024 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-60320242018-07-09 Ultrafiltration and size exclusion chromatography combined with asymmetrical-flow field-flow fractionation for the isolation and characterisation of extracellular vesicles from urine Oeyen, Eline Van Mol, Kurt Baggerman, Geert Willems, Hanny Boonen, Kurt Rolfo, Christian Pauwels, Patrick Jacobs, An Schildermans, Karin Cho, William C Mertens, Inge J Extracell Vesicles Research Article Extracellular vesicles (EVs) have a great potential in clinical applications. However, their isolation from different bodily fluids and their characterisation are currently not optimal or standardised. Here, we report the results of examining the performance of ultrafiltration combined with size exclusion chromatography (UF-SEC) to isolate EVs from urine. The results reveal that UF-SEC is an efficient method and provides high purity. Furthermore, we introduce asymmetrical-flow field-flow fractionation coupled with a UV detector and multi-angle light-scattering detector (AF4/UV-MALS) as a characterisation method and compare it with current methods. We demonstrate that AF4/UV-MALS is a straightforward and reproducible method for determining size, amount and purity of isolated urinary EVs. Taylor & Francis 2018-07-03 /pmc/articles/PMC6032024/ /pubmed/29988836 http://dx.doi.org/10.1080/20013078.2018.1490143 Text en © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Oeyen, Eline Van Mol, Kurt Baggerman, Geert Willems, Hanny Boonen, Kurt Rolfo, Christian Pauwels, Patrick Jacobs, An Schildermans, Karin Cho, William C Mertens, Inge Ultrafiltration and size exclusion chromatography combined with asymmetrical-flow field-flow fractionation for the isolation and characterisation of extracellular vesicles from urine |
title | Ultrafiltration and size exclusion chromatography combined with asymmetrical-flow field-flow fractionation for the isolation and characterisation of extracellular vesicles from urine |
title_full | Ultrafiltration and size exclusion chromatography combined with asymmetrical-flow field-flow fractionation for the isolation and characterisation of extracellular vesicles from urine |
title_fullStr | Ultrafiltration and size exclusion chromatography combined with asymmetrical-flow field-flow fractionation for the isolation and characterisation of extracellular vesicles from urine |
title_full_unstemmed | Ultrafiltration and size exclusion chromatography combined with asymmetrical-flow field-flow fractionation for the isolation and characterisation of extracellular vesicles from urine |
title_short | Ultrafiltration and size exclusion chromatography combined with asymmetrical-flow field-flow fractionation for the isolation and characterisation of extracellular vesicles from urine |
title_sort | ultrafiltration and size exclusion chromatography combined with asymmetrical-flow field-flow fractionation for the isolation and characterisation of extracellular vesicles from urine |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6032024/ https://www.ncbi.nlm.nih.gov/pubmed/29988836 http://dx.doi.org/10.1080/20013078.2018.1490143 |
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