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Tumor Size-Dependent Anticancer Efficacy of Chlorin Derivatives for Photodynamic Therapy
Photodynamic therapy (PDT) with a suitable photosensitizer molecule is a promising anticancer treatment. We evaluated two chlorin molecules as potential photosensitizers, methyl pyropheophorbide a (MPPa) and N-methoxyl purpurinimide (NMPi), against A549 human lung adenocarcinoma cells in vitro as we...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6032057/ https://www.ncbi.nlm.nih.gov/pubmed/29844257 http://dx.doi.org/10.3390/ijms19061596 |
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author | Chang, Ji-Eun Liu, Yang Lee, Tae Heon Lee, Woo Kyoung Yoon, Il Kim, Kwhanmien |
author_facet | Chang, Ji-Eun Liu, Yang Lee, Tae Heon Lee, Woo Kyoung Yoon, Il Kim, Kwhanmien |
author_sort | Chang, Ji-Eun |
collection | PubMed |
description | Photodynamic therapy (PDT) with a suitable photosensitizer molecule is a promising anticancer treatment. We evaluated two chlorin molecules as potential photosensitizers, methyl pyropheophorbide a (MPPa) and N-methoxyl purpurinimide (NMPi), against A549 human lung adenocarcinoma cells in vitro as well as in A549 tumor-bearing mice in vivo. Cell viability, microscopy, and fluorescence-activated cell sorting (FACS) analyses were performed for the in vitro studies. MPPa and NMPi showed high phototoxicity in vitro, which was dependent on the concentration of the photosensitizers as well as the light irradiation time. In the animal study, tumor volume change, tumor surface alterations, and hematoxylin & eosin (H&E) and terminal deoxyribonucleotidyl transferse-mediated dUTP nick-end labelling (TUNEL) staining analyses were performed and compared between small (tumor volume of <50 mm(3)) and large (tumor volume of >50 mm(3)) size of initial tumors. MPPa and NMPi showed high anticancer efficacy against small-size tumors, indicating that early treatment with PDT is effective. Especially, repeated two times PDT with NMPi allowed almost complete eradication against small-size tumors. However, MPPa and NMPi were not effective against large-size tumors. In conclusion, the two chlorin derivatives, MPPa and NMPi, show good anticancer efficacy as promising photosensitizers for PDT in vitro and in vivo. Moreover, their activity in vivo was significantly dependent on the initial tumor size in mice, which confirms the importance of early cancer treatment. |
format | Online Article Text |
id | pubmed-6032057 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-60320572018-07-13 Tumor Size-Dependent Anticancer Efficacy of Chlorin Derivatives for Photodynamic Therapy Chang, Ji-Eun Liu, Yang Lee, Tae Heon Lee, Woo Kyoung Yoon, Il Kim, Kwhanmien Int J Mol Sci Article Photodynamic therapy (PDT) with a suitable photosensitizer molecule is a promising anticancer treatment. We evaluated two chlorin molecules as potential photosensitizers, methyl pyropheophorbide a (MPPa) and N-methoxyl purpurinimide (NMPi), against A549 human lung adenocarcinoma cells in vitro as well as in A549 tumor-bearing mice in vivo. Cell viability, microscopy, and fluorescence-activated cell sorting (FACS) analyses were performed for the in vitro studies. MPPa and NMPi showed high phototoxicity in vitro, which was dependent on the concentration of the photosensitizers as well as the light irradiation time. In the animal study, tumor volume change, tumor surface alterations, and hematoxylin & eosin (H&E) and terminal deoxyribonucleotidyl transferse-mediated dUTP nick-end labelling (TUNEL) staining analyses were performed and compared between small (tumor volume of <50 mm(3)) and large (tumor volume of >50 mm(3)) size of initial tumors. MPPa and NMPi showed high anticancer efficacy against small-size tumors, indicating that early treatment with PDT is effective. Especially, repeated two times PDT with NMPi allowed almost complete eradication against small-size tumors. However, MPPa and NMPi were not effective against large-size tumors. In conclusion, the two chlorin derivatives, MPPa and NMPi, show good anticancer efficacy as promising photosensitizers for PDT in vitro and in vivo. Moreover, their activity in vivo was significantly dependent on the initial tumor size in mice, which confirms the importance of early cancer treatment. MDPI 2018-05-29 /pmc/articles/PMC6032057/ /pubmed/29844257 http://dx.doi.org/10.3390/ijms19061596 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chang, Ji-Eun Liu, Yang Lee, Tae Heon Lee, Woo Kyoung Yoon, Il Kim, Kwhanmien Tumor Size-Dependent Anticancer Efficacy of Chlorin Derivatives for Photodynamic Therapy |
title | Tumor Size-Dependent Anticancer Efficacy of Chlorin Derivatives for Photodynamic Therapy |
title_full | Tumor Size-Dependent Anticancer Efficacy of Chlorin Derivatives for Photodynamic Therapy |
title_fullStr | Tumor Size-Dependent Anticancer Efficacy of Chlorin Derivatives for Photodynamic Therapy |
title_full_unstemmed | Tumor Size-Dependent Anticancer Efficacy of Chlorin Derivatives for Photodynamic Therapy |
title_short | Tumor Size-Dependent Anticancer Efficacy of Chlorin Derivatives for Photodynamic Therapy |
title_sort | tumor size-dependent anticancer efficacy of chlorin derivatives for photodynamic therapy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6032057/ https://www.ncbi.nlm.nih.gov/pubmed/29844257 http://dx.doi.org/10.3390/ijms19061596 |
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