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Design of a Seed-Specific Chimeric Promoter with a Modified Expression Profile to Improve Seed Oil Content

Increasing the yield of plant oil is an important objective to meet the demand for sustainable resources and energy. Some attempts to enhance the expression of genes involved in oil synthesis in seeds have succeeded in increasing oil content. In many cases, the promoters of seed-storage protein gene...

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Autores principales: Aoyagi, Toshihiro, Kobayashi, Masaya, Kozaki, Akiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6032214/
https://www.ncbi.nlm.nih.gov/pubmed/29874815
http://dx.doi.org/10.3390/ijms19061667
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author Aoyagi, Toshihiro
Kobayashi, Masaya
Kozaki, Akiko
author_facet Aoyagi, Toshihiro
Kobayashi, Masaya
Kozaki, Akiko
author_sort Aoyagi, Toshihiro
collection PubMed
description Increasing the yield of plant oil is an important objective to meet the demand for sustainable resources and energy. Some attempts to enhance the expression of genes involved in oil synthesis in seeds have succeeded in increasing oil content. In many cases, the promoters of seed-storage protein genes have been used as seed-specific promoters. However, conventional promoters are developmentally regulated and their expression periods are limited. We constructed a chimeric promoter that starts to express in the early stage of seed development, and high-level expression is retained until the later stage by connecting the promoters of the biotin carboxyl carrier protein 2 (BCCP2) gene encoding the BCCP2 subunit of acetyl-CoA carboxylase and the fatty acid elongase 1 (FAE1) gene from Arabidopsis. The constructed promoter was ligated upstream of the TAG1 gene encoding diacylglycerol acyltransferase 1 and introduced into Arabidopsis. Seeds from transgenic plants carrying AtTAG1 under the control of the chimeric promoter showed increased oil content (up by 18–73%) compared with wild-type seeds. The novel expression profile of the chimeric promoter showed that this could be a promising strategy to manipulate the content of seed-storage oils and other compounds.
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spelling pubmed-60322142018-07-13 Design of a Seed-Specific Chimeric Promoter with a Modified Expression Profile to Improve Seed Oil Content Aoyagi, Toshihiro Kobayashi, Masaya Kozaki, Akiko Int J Mol Sci Article Increasing the yield of plant oil is an important objective to meet the demand for sustainable resources and energy. Some attempts to enhance the expression of genes involved in oil synthesis in seeds have succeeded in increasing oil content. In many cases, the promoters of seed-storage protein genes have been used as seed-specific promoters. However, conventional promoters are developmentally regulated and their expression periods are limited. We constructed a chimeric promoter that starts to express in the early stage of seed development, and high-level expression is retained until the later stage by connecting the promoters of the biotin carboxyl carrier protein 2 (BCCP2) gene encoding the BCCP2 subunit of acetyl-CoA carboxylase and the fatty acid elongase 1 (FAE1) gene from Arabidopsis. The constructed promoter was ligated upstream of the TAG1 gene encoding diacylglycerol acyltransferase 1 and introduced into Arabidopsis. Seeds from transgenic plants carrying AtTAG1 under the control of the chimeric promoter showed increased oil content (up by 18–73%) compared with wild-type seeds. The novel expression profile of the chimeric promoter showed that this could be a promising strategy to manipulate the content of seed-storage oils and other compounds. MDPI 2018-06-05 /pmc/articles/PMC6032214/ /pubmed/29874815 http://dx.doi.org/10.3390/ijms19061667 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aoyagi, Toshihiro
Kobayashi, Masaya
Kozaki, Akiko
Design of a Seed-Specific Chimeric Promoter with a Modified Expression Profile to Improve Seed Oil Content
title Design of a Seed-Specific Chimeric Promoter with a Modified Expression Profile to Improve Seed Oil Content
title_full Design of a Seed-Specific Chimeric Promoter with a Modified Expression Profile to Improve Seed Oil Content
title_fullStr Design of a Seed-Specific Chimeric Promoter with a Modified Expression Profile to Improve Seed Oil Content
title_full_unstemmed Design of a Seed-Specific Chimeric Promoter with a Modified Expression Profile to Improve Seed Oil Content
title_short Design of a Seed-Specific Chimeric Promoter with a Modified Expression Profile to Improve Seed Oil Content
title_sort design of a seed-specific chimeric promoter with a modified expression profile to improve seed oil content
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6032214/
https://www.ncbi.nlm.nih.gov/pubmed/29874815
http://dx.doi.org/10.3390/ijms19061667
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AT kozakiakiko designofaseedspecificchimericpromoterwithamodifiedexpressionprofiletoimproveseedoilcontent