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Comparison of Three Extraction Approaches for the Isolation of Neurotransmitters from Rat Brain Samples
The determination of neurotransmitters (NTs) as relevant potential biomarkers in the study of various central nervous system (CNS) pathologies has been demonstrated. Knowing that NTs-related diseases mostly occupy individual regions of the nervous system, as observed, for instance, in neurodegenerat...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6032232/ https://www.ncbi.nlm.nih.gov/pubmed/29882927 http://dx.doi.org/10.3390/ijms19061560 |
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author | Miękus, Natalia Olędzka, Ilona Harshkova, Darya Liakh, Ivan Plenis, Alina Kowalski, Piotr Bączek, Tomasz |
author_facet | Miękus, Natalia Olędzka, Ilona Harshkova, Darya Liakh, Ivan Plenis, Alina Kowalski, Piotr Bączek, Tomasz |
author_sort | Miękus, Natalia |
collection | PubMed |
description | The determination of neurotransmitters (NTs) as relevant potential biomarkers in the study of various central nervous system (CNS) pathologies has been demonstrated. Knowing that NTs-related diseases mostly occupy individual regions of the nervous system, as observed, for instance, in neurodegenerative diseases (Alzheimer’s and Parkinson’s Diseases), the analysis of brain slices is preferred to whole-brain analysis. In this report, we present sample preparation approaches, such as solid-phase extraction, solid-phase microextraction, and dispersive liquid–liquid microextraction, and discuss the pitfalls and advantages of each extraction method. The ionic liquid (1-ethyl-3-methylimidazolium tetrafluoroborate)-assisted solid-phase microextraction (IL-SPME) is found to be, in our research, the relevant step towards the simultaneous determination of six NTs, namely, dopamine (DA), adrenaline (A), noradrenaline (NA), serotonin (5-HT), l-tryptophan (l-Trp), l-tyrosine (l-Tyr) in rat brain samples. The development of a novel bioanalytical technique for the evaluation of biomarkers in the context of green chemistry might be accelerated just with the use of IL, and this approach can be considered an advantageous strategy. |
format | Online Article Text |
id | pubmed-6032232 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-60322322018-07-13 Comparison of Three Extraction Approaches for the Isolation of Neurotransmitters from Rat Brain Samples Miękus, Natalia Olędzka, Ilona Harshkova, Darya Liakh, Ivan Plenis, Alina Kowalski, Piotr Bączek, Tomasz Int J Mol Sci Communication The determination of neurotransmitters (NTs) as relevant potential biomarkers in the study of various central nervous system (CNS) pathologies has been demonstrated. Knowing that NTs-related diseases mostly occupy individual regions of the nervous system, as observed, for instance, in neurodegenerative diseases (Alzheimer’s and Parkinson’s Diseases), the analysis of brain slices is preferred to whole-brain analysis. In this report, we present sample preparation approaches, such as solid-phase extraction, solid-phase microextraction, and dispersive liquid–liquid microextraction, and discuss the pitfalls and advantages of each extraction method. The ionic liquid (1-ethyl-3-methylimidazolium tetrafluoroborate)-assisted solid-phase microextraction (IL-SPME) is found to be, in our research, the relevant step towards the simultaneous determination of six NTs, namely, dopamine (DA), adrenaline (A), noradrenaline (NA), serotonin (5-HT), l-tryptophan (l-Trp), l-tyrosine (l-Tyr) in rat brain samples. The development of a novel bioanalytical technique for the evaluation of biomarkers in the context of green chemistry might be accelerated just with the use of IL, and this approach can be considered an advantageous strategy. MDPI 2018-05-24 /pmc/articles/PMC6032232/ /pubmed/29882927 http://dx.doi.org/10.3390/ijms19061560 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Miękus, Natalia Olędzka, Ilona Harshkova, Darya Liakh, Ivan Plenis, Alina Kowalski, Piotr Bączek, Tomasz Comparison of Three Extraction Approaches for the Isolation of Neurotransmitters from Rat Brain Samples |
title | Comparison of Three Extraction Approaches for the Isolation of Neurotransmitters from Rat Brain Samples |
title_full | Comparison of Three Extraction Approaches for the Isolation of Neurotransmitters from Rat Brain Samples |
title_fullStr | Comparison of Three Extraction Approaches for the Isolation of Neurotransmitters from Rat Brain Samples |
title_full_unstemmed | Comparison of Three Extraction Approaches for the Isolation of Neurotransmitters from Rat Brain Samples |
title_short | Comparison of Three Extraction Approaches for the Isolation of Neurotransmitters from Rat Brain Samples |
title_sort | comparison of three extraction approaches for the isolation of neurotransmitters from rat brain samples |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6032232/ https://www.ncbi.nlm.nih.gov/pubmed/29882927 http://dx.doi.org/10.3390/ijms19061560 |
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