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pSTAT3 Levels Have Divergent Expression Patterns and Associations with Survival in Squamous Cell Carcinoma and Adenocarcinoma of the Oesophagus

Signal transducers and activator of transcription (STAT)-3 is activated in cancers, where it promotes growth, inflammation, angiogenesis, and inhibits apoptosis. Tissue microarrays were generated using tissues from 154 patients, with oesophageal adenocarcinoma (OAC) (n = 116) or squamous cell carcin...

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Autores principales: O’ Sullivan, Katie E., Michielsen, Adriana J., O’ Regan, Esther, Cathcart, Mary C., Moore, Gillian, Breen, Eamon, Segurado, Ricardo, Reynolds, John V., Lysaght, Joanne, O’ Sullivan, Jacintha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6032321/
https://www.ncbi.nlm.nih.gov/pubmed/29890775
http://dx.doi.org/10.3390/ijms19061720
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author O’ Sullivan, Katie E.
Michielsen, Adriana J.
O’ Regan, Esther
Cathcart, Mary C.
Moore, Gillian
Breen, Eamon
Segurado, Ricardo
Reynolds, John V.
Lysaght, Joanne
O’ Sullivan, Jacintha
author_facet O’ Sullivan, Katie E.
Michielsen, Adriana J.
O’ Regan, Esther
Cathcart, Mary C.
Moore, Gillian
Breen, Eamon
Segurado, Ricardo
Reynolds, John V.
Lysaght, Joanne
O’ Sullivan, Jacintha
author_sort O’ Sullivan, Katie E.
collection PubMed
description Signal transducers and activator of transcription (STAT)-3 is activated in cancers, where it promotes growth, inflammation, angiogenesis, and inhibits apoptosis. Tissue microarrays were generated using tissues from 154 patients, with oesophageal adenocarcinoma (OAC) (n = 116) or squamous cell carcinoma (SCC) (n = 38) tumours. The tissues were stained for pSTAT3 and IL-6R using immunohistochemistry. The OE33 (OAC) and OE21 (SCC) cell lines were treated with the STAT3 inhibitor, STATTIC. The Univariate cox regression analysis revealed that a positive pSTAT3 in SCC was adversely associated with survival (Hazard ratio (HR) 6.382, 95% CI 1.266–32.184), while a protective effect was demonstrated with the higher pSTAT3 levels in OAC epithelium (HR 0.74, 95% CI 0.574–0.953). The IL-6R intensity levels were higher in the SCC tumours compared with the OAC tumours for the core and leading edge tumour tissue. The pSTAT3 levels correlated positively with the IL-6R levels in both the OAC and SCC. The treatment of OE21 and OE33 cells with the STAT3 inhibitor STATTIC in vitro resulted in decreased survival, proliferation, migration, and increased apoptosis. The pSTAT3 expression was associated with adverse survival in SCC, but not in the OAC patients. The inhibition of STAT3 in both of the tumour subtypes resulted in alterations in the survival, proliferation, migration, and apoptosis, suggesting a potential role for therapeutically targeting STAT3.
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spelling pubmed-60323212018-07-13 pSTAT3 Levels Have Divergent Expression Patterns and Associations with Survival in Squamous Cell Carcinoma and Adenocarcinoma of the Oesophagus O’ Sullivan, Katie E. Michielsen, Adriana J. O’ Regan, Esther Cathcart, Mary C. Moore, Gillian Breen, Eamon Segurado, Ricardo Reynolds, John V. Lysaght, Joanne O’ Sullivan, Jacintha Int J Mol Sci Article Signal transducers and activator of transcription (STAT)-3 is activated in cancers, where it promotes growth, inflammation, angiogenesis, and inhibits apoptosis. Tissue microarrays were generated using tissues from 154 patients, with oesophageal adenocarcinoma (OAC) (n = 116) or squamous cell carcinoma (SCC) (n = 38) tumours. The tissues were stained for pSTAT3 and IL-6R using immunohistochemistry. The OE33 (OAC) and OE21 (SCC) cell lines were treated with the STAT3 inhibitor, STATTIC. The Univariate cox regression analysis revealed that a positive pSTAT3 in SCC was adversely associated with survival (Hazard ratio (HR) 6.382, 95% CI 1.266–32.184), while a protective effect was demonstrated with the higher pSTAT3 levels in OAC epithelium (HR 0.74, 95% CI 0.574–0.953). The IL-6R intensity levels were higher in the SCC tumours compared with the OAC tumours for the core and leading edge tumour tissue. The pSTAT3 levels correlated positively with the IL-6R levels in both the OAC and SCC. The treatment of OE21 and OE33 cells with the STAT3 inhibitor STATTIC in vitro resulted in decreased survival, proliferation, migration, and increased apoptosis. The pSTAT3 expression was associated with adverse survival in SCC, but not in the OAC patients. The inhibition of STAT3 in both of the tumour subtypes resulted in alterations in the survival, proliferation, migration, and apoptosis, suggesting a potential role for therapeutically targeting STAT3. MDPI 2018-06-10 /pmc/articles/PMC6032321/ /pubmed/29890775 http://dx.doi.org/10.3390/ijms19061720 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
O’ Sullivan, Katie E.
Michielsen, Adriana J.
O’ Regan, Esther
Cathcart, Mary C.
Moore, Gillian
Breen, Eamon
Segurado, Ricardo
Reynolds, John V.
Lysaght, Joanne
O’ Sullivan, Jacintha
pSTAT3 Levels Have Divergent Expression Patterns and Associations with Survival in Squamous Cell Carcinoma and Adenocarcinoma of the Oesophagus
title pSTAT3 Levels Have Divergent Expression Patterns and Associations with Survival in Squamous Cell Carcinoma and Adenocarcinoma of the Oesophagus
title_full pSTAT3 Levels Have Divergent Expression Patterns and Associations with Survival in Squamous Cell Carcinoma and Adenocarcinoma of the Oesophagus
title_fullStr pSTAT3 Levels Have Divergent Expression Patterns and Associations with Survival in Squamous Cell Carcinoma and Adenocarcinoma of the Oesophagus
title_full_unstemmed pSTAT3 Levels Have Divergent Expression Patterns and Associations with Survival in Squamous Cell Carcinoma and Adenocarcinoma of the Oesophagus
title_short pSTAT3 Levels Have Divergent Expression Patterns and Associations with Survival in Squamous Cell Carcinoma and Adenocarcinoma of the Oesophagus
title_sort pstat3 levels have divergent expression patterns and associations with survival in squamous cell carcinoma and adenocarcinoma of the oesophagus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6032321/
https://www.ncbi.nlm.nih.gov/pubmed/29890775
http://dx.doi.org/10.3390/ijms19061720
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