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Very Small Embryonic-Like Stem Cells, Endothelial Progenitor Cells, and Different Monocyte Subsets Are Effectively Mobilized in Acute Lymphoblastic Leukemia Patients after G-CSF Treatment

BACKGROUND: Acute lymphoblastic leukemia (ALL) is a malignant disease of lymphoid progenitor cells. ALL chemotherapy is associated with numerous side effects including neutropenia that is routinely prevented by the administration of growth factors such as granulocyte colony-stimulating factor (G-CSF...

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Autores principales: Eljaszewicz, Andrzej, Bolkun, Lukasz, Grubczak, Kamil, Rusak, Malgorzata, Wasiluk, Tomasz, Dabrowska, Milena, Radziwon, Piotr, Marlicz, Wojciech, Kamiński, Karol, Kloczko, Janusz, Moniuszko, Marcin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6032642/
https://www.ncbi.nlm.nih.gov/pubmed/30034479
http://dx.doi.org/10.1155/2018/1943980
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author Eljaszewicz, Andrzej
Bolkun, Lukasz
Grubczak, Kamil
Rusak, Malgorzata
Wasiluk, Tomasz
Dabrowska, Milena
Radziwon, Piotr
Marlicz, Wojciech
Kamiński, Karol
Kloczko, Janusz
Moniuszko, Marcin
author_facet Eljaszewicz, Andrzej
Bolkun, Lukasz
Grubczak, Kamil
Rusak, Malgorzata
Wasiluk, Tomasz
Dabrowska, Milena
Radziwon, Piotr
Marlicz, Wojciech
Kamiński, Karol
Kloczko, Janusz
Moniuszko, Marcin
author_sort Eljaszewicz, Andrzej
collection PubMed
description BACKGROUND: Acute lymphoblastic leukemia (ALL) is a malignant disease of lymphoid progenitor cells. ALL chemotherapy is associated with numerous side effects including neutropenia that is routinely prevented by the administration of growth factors such as granulocyte colony-stimulating factor (G-CSF). To date, the effects of G-CSF treatment on the level of mobilization of different stem and progenitor cells in ALL patients subjected to clinically effective chemotherapy have not been fully elucidated. Therefore, in this study we aimed to assess the effect of administration of G-CSF to ALL patients on mobilization of other than hematopoietic stem cell (HSCs) subsets, namely, very small embryonic-like stem cells (VSELs), endothelial progenitor cells (EPCs), and different monocyte subsets. METHODS: We used multicolor flow cytometry to quantitate numbers of CD34+ cells, hematopoietic stem cells (HSCs), VSELs, EPCs, and different monocyte subsets in the peripheral blood of ALL patients and normal age-matched blood donors. RESULTS: We showed that ALL patients following chemotherapy, when compared to healthy donors, presented with significantly lower numbers of CD34+ cells, HSCs, VSELs, and CD14+ monocytes, but not EPCs. Moreover, we found that G-CSF administration induced effective mobilization of all the abovementioned progenitor and stem cell subsets with high regenerative and proangiogenic potential. CONCLUSION: These findings contribute to better understanding the beneficial clinical effect of G-CSF administration in ALL patients following successful chemotherapy.
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spelling pubmed-60326422018-07-22 Very Small Embryonic-Like Stem Cells, Endothelial Progenitor Cells, and Different Monocyte Subsets Are Effectively Mobilized in Acute Lymphoblastic Leukemia Patients after G-CSF Treatment Eljaszewicz, Andrzej Bolkun, Lukasz Grubczak, Kamil Rusak, Malgorzata Wasiluk, Tomasz Dabrowska, Milena Radziwon, Piotr Marlicz, Wojciech Kamiński, Karol Kloczko, Janusz Moniuszko, Marcin Stem Cells Int Research Article BACKGROUND: Acute lymphoblastic leukemia (ALL) is a malignant disease of lymphoid progenitor cells. ALL chemotherapy is associated with numerous side effects including neutropenia that is routinely prevented by the administration of growth factors such as granulocyte colony-stimulating factor (G-CSF). To date, the effects of G-CSF treatment on the level of mobilization of different stem and progenitor cells in ALL patients subjected to clinically effective chemotherapy have not been fully elucidated. Therefore, in this study we aimed to assess the effect of administration of G-CSF to ALL patients on mobilization of other than hematopoietic stem cell (HSCs) subsets, namely, very small embryonic-like stem cells (VSELs), endothelial progenitor cells (EPCs), and different monocyte subsets. METHODS: We used multicolor flow cytometry to quantitate numbers of CD34+ cells, hematopoietic stem cells (HSCs), VSELs, EPCs, and different monocyte subsets in the peripheral blood of ALL patients and normal age-matched blood donors. RESULTS: We showed that ALL patients following chemotherapy, when compared to healthy donors, presented with significantly lower numbers of CD34+ cells, HSCs, VSELs, and CD14+ monocytes, but not EPCs. Moreover, we found that G-CSF administration induced effective mobilization of all the abovementioned progenitor and stem cell subsets with high regenerative and proangiogenic potential. CONCLUSION: These findings contribute to better understanding the beneficial clinical effect of G-CSF administration in ALL patients following successful chemotherapy. Hindawi 2018-06-21 /pmc/articles/PMC6032642/ /pubmed/30034479 http://dx.doi.org/10.1155/2018/1943980 Text en Copyright © 2018 Andrzej Eljaszewicz et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Eljaszewicz, Andrzej
Bolkun, Lukasz
Grubczak, Kamil
Rusak, Malgorzata
Wasiluk, Tomasz
Dabrowska, Milena
Radziwon, Piotr
Marlicz, Wojciech
Kamiński, Karol
Kloczko, Janusz
Moniuszko, Marcin
Very Small Embryonic-Like Stem Cells, Endothelial Progenitor Cells, and Different Monocyte Subsets Are Effectively Mobilized in Acute Lymphoblastic Leukemia Patients after G-CSF Treatment
title Very Small Embryonic-Like Stem Cells, Endothelial Progenitor Cells, and Different Monocyte Subsets Are Effectively Mobilized in Acute Lymphoblastic Leukemia Patients after G-CSF Treatment
title_full Very Small Embryonic-Like Stem Cells, Endothelial Progenitor Cells, and Different Monocyte Subsets Are Effectively Mobilized in Acute Lymphoblastic Leukemia Patients after G-CSF Treatment
title_fullStr Very Small Embryonic-Like Stem Cells, Endothelial Progenitor Cells, and Different Monocyte Subsets Are Effectively Mobilized in Acute Lymphoblastic Leukemia Patients after G-CSF Treatment
title_full_unstemmed Very Small Embryonic-Like Stem Cells, Endothelial Progenitor Cells, and Different Monocyte Subsets Are Effectively Mobilized in Acute Lymphoblastic Leukemia Patients after G-CSF Treatment
title_short Very Small Embryonic-Like Stem Cells, Endothelial Progenitor Cells, and Different Monocyte Subsets Are Effectively Mobilized in Acute Lymphoblastic Leukemia Patients after G-CSF Treatment
title_sort very small embryonic-like stem cells, endothelial progenitor cells, and different monocyte subsets are effectively mobilized in acute lymphoblastic leukemia patients after g-csf treatment
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6032642/
https://www.ncbi.nlm.nih.gov/pubmed/30034479
http://dx.doi.org/10.1155/2018/1943980
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