Optogenetic Control of Voltage‐Gated Calcium Channels

Voltage‐gated Ca(2+) (Ca(V)) channels mediate Ca(2+) entry into excitable cells to regulate a myriad of cellular events following membrane depolarization. We report the engineering of RGK GTPases, a class of genetically encoded Ca(V) channel modulators, to enable photo‐tunable modulation of Ca(V) ch...

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Detalles Bibliográficos
Autores principales: Ma, Guolin, Liu, Jindou, Ke, Yuepeng, Liu, Xin, Li, Minyong, Wang, Fen, Han, Gang, Huang, Yun, Wang, Youjun, Zhou, Yubin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6032918/
https://www.ncbi.nlm.nih.gov/pubmed/29569306
http://dx.doi.org/10.1002/anie.201713080
Descripción
Sumario:Voltage‐gated Ca(2+) (Ca(V)) channels mediate Ca(2+) entry into excitable cells to regulate a myriad of cellular events following membrane depolarization. We report the engineering of RGK GTPases, a class of genetically encoded Ca(V) channel modulators, to enable photo‐tunable modulation of Ca(V) channel activity in excitable mammalian cells. This optogenetic tool (designated optoRGK) tailored for Ca(V) channels could find broad applications in interrogating a wide range of Ca(V)‐mediated physiological processes.

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