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Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin

Ovine footrot is a degenerative disease of sheep feet leading to the separation of hoof-horn from the underlying skin and lameness. This study quantitatively examined histological features of the ovine interdigital skin as well as their relationship with pro-inflammatory cytokine (IL-1β) and virulen...

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Autores principales: Agbaje, Michael, Rutland, Catrin S., Maboni, Grazieli, Blanchard, Adam, Bexon, Melissa, Stewart, Ceri, Jones, Michael A., Totemeyer, Sabine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6033080/
https://www.ncbi.nlm.nih.gov/pubmed/30002960
http://dx.doi.org/10.7717/peerj.5097
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author Agbaje, Michael
Rutland, Catrin S.
Maboni, Grazieli
Blanchard, Adam
Bexon, Melissa
Stewart, Ceri
Jones, Michael A.
Totemeyer, Sabine
author_facet Agbaje, Michael
Rutland, Catrin S.
Maboni, Grazieli
Blanchard, Adam
Bexon, Melissa
Stewart, Ceri
Jones, Michael A.
Totemeyer, Sabine
author_sort Agbaje, Michael
collection PubMed
description Ovine footrot is a degenerative disease of sheep feet leading to the separation of hoof-horn from the underlying skin and lameness. This study quantitatively examined histological features of the ovine interdigital skin as well as their relationship with pro-inflammatory cytokine (IL-1β) and virulent Dichelobacter nodosus in footrot. From 55 healthy and 30 footrot ovine feet, parallel biopsies (one fixed for histology) were collected post-slaughter and analysed for lesions and histopathological analysis using haematoxylin and eosin and Periodic Acid-Schiff. Histological lesions were similar in both conditions while inflammatory scores mirror IL-1β expression levels. Increased inflammatory score corresponded with high virulent D. nodosus load and was significant (p < 0.0001) in footrot feet with an inflammatory score of 3 compared to scores 1 and 2. In addition, in contrast to healthy tissues, localisation of eubacterial load extended beyond follicular depths in footrot samples. The novel inflammatory cell infiltration scoring system in this study may be used to grade inflammatory response in the ovine feet and demonstrated an association between severity of inflammatory response and increased virulent D. nodosus load.
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spelling pubmed-60330802018-07-12 Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin Agbaje, Michael Rutland, Catrin S. Maboni, Grazieli Blanchard, Adam Bexon, Melissa Stewart, Ceri Jones, Michael A. Totemeyer, Sabine PeerJ Agricultural Science Ovine footrot is a degenerative disease of sheep feet leading to the separation of hoof-horn from the underlying skin and lameness. This study quantitatively examined histological features of the ovine interdigital skin as well as their relationship with pro-inflammatory cytokine (IL-1β) and virulent Dichelobacter nodosus in footrot. From 55 healthy and 30 footrot ovine feet, parallel biopsies (one fixed for histology) were collected post-slaughter and analysed for lesions and histopathological analysis using haematoxylin and eosin and Periodic Acid-Schiff. Histological lesions were similar in both conditions while inflammatory scores mirror IL-1β expression levels. Increased inflammatory score corresponded with high virulent D. nodosus load and was significant (p < 0.0001) in footrot feet with an inflammatory score of 3 compared to scores 1 and 2. In addition, in contrast to healthy tissues, localisation of eubacterial load extended beyond follicular depths in footrot samples. The novel inflammatory cell infiltration scoring system in this study may be used to grade inflammatory response in the ovine feet and demonstrated an association between severity of inflammatory response and increased virulent D. nodosus load. PeerJ Inc. 2018-07-02 /pmc/articles/PMC6033080/ /pubmed/30002960 http://dx.doi.org/10.7717/peerj.5097 Text en ©2018 Agbaje et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Agbaje, Michael
Rutland, Catrin S.
Maboni, Grazieli
Blanchard, Adam
Bexon, Melissa
Stewart, Ceri
Jones, Michael A.
Totemeyer, Sabine
Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin
title Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin
title_full Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin
title_fullStr Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin
title_full_unstemmed Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin
title_short Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin
title_sort novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6033080/
https://www.ncbi.nlm.nih.gov/pubmed/30002960
http://dx.doi.org/10.7717/peerj.5097
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