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Dynamic changes in morphology, gene expression and microbiome in the jejunum of compensatory‐growth rats induced by protein restriction

We previously reported that protein‐restricted rats experienced compensatory growth when they were switched to a normal protein diet (NPD). This study aimed to investigate the changes in gene expression and microbiome in the jejunum of compensatory‐growth rats. Weaned Sprague‐Dawley rats were assign...

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Autores principales: Zhu, Yizhi, Shi, Chao, Niu, Qingyan, Wang, Jing, Zhu, Weiyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6033190/
https://www.ncbi.nlm.nih.gov/pubmed/29633535
http://dx.doi.org/10.1111/1751-7915.13266
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author Zhu, Yizhi
Shi, Chao
Niu, Qingyan
Wang, Jing
Zhu, Weiyun
author_facet Zhu, Yizhi
Shi, Chao
Niu, Qingyan
Wang, Jing
Zhu, Weiyun
author_sort Zhu, Yizhi
collection PubMed
description We previously reported that protein‐restricted rats experienced compensatory growth when they were switched to a normal protein diet (NPD). This study aimed to investigate the changes in gene expression and microbiome in the jejunum of compensatory‐growth rats. Weaned Sprague‐Dawley rats were assigned to an N group, an LN group and an L group. The rats in the L and N groups were fed a low protein diet (LPD) and the NPD respectively. The rats in the LN group were fed with the LPD for 2 weeks, followed by the NPD. The experiment lasted 70 days, and the rats were sacrificed for sampling on days 14, 28 and 70 to determine the jejunal morphology, microbiome and gene expression related to digestive, absorptive and barrier function. The results showed that, although rats in the LN group had temporarily impaired morphology and gene expression in the jejunum on day 14 in response to the LPD, they had improved jejunal morphology and gene expression related to jejunal function on day 28 compared to rats in the N group. This improvement might promote compensatory growth of rats. However, lower expression of genes related to nutrient absorption and undifferentiated villous height (VH) were observed in the jejunum of rats in the LN group on day 70. In contrast, rats in the L group had lower VH on day 28 and day 70, while the expression of absorptive genes increased on day 28 compared to rats in the N group. Additionally, dramatic microbial changes in the jejunum of compensatory‐growth rats were observed, principally for Lactobacillus, Streptococcus, Corynebacterium and Staphylococcus. Moreover, the abundance of Lactobacillus, Streptococcus, Corynebacterium and Staphylococcus significantly correlated with gene expression in the jejunum as revealed by the correlation analysis.
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spelling pubmed-60331902018-07-13 Dynamic changes in morphology, gene expression and microbiome in the jejunum of compensatory‐growth rats induced by protein restriction Zhu, Yizhi Shi, Chao Niu, Qingyan Wang, Jing Zhu, Weiyun Microb Biotechnol Research Articles We previously reported that protein‐restricted rats experienced compensatory growth when they were switched to a normal protein diet (NPD). This study aimed to investigate the changes in gene expression and microbiome in the jejunum of compensatory‐growth rats. Weaned Sprague‐Dawley rats were assigned to an N group, an LN group and an L group. The rats in the L and N groups were fed a low protein diet (LPD) and the NPD respectively. The rats in the LN group were fed with the LPD for 2 weeks, followed by the NPD. The experiment lasted 70 days, and the rats were sacrificed for sampling on days 14, 28 and 70 to determine the jejunal morphology, microbiome and gene expression related to digestive, absorptive and barrier function. The results showed that, although rats in the LN group had temporarily impaired morphology and gene expression in the jejunum on day 14 in response to the LPD, they had improved jejunal morphology and gene expression related to jejunal function on day 28 compared to rats in the N group. This improvement might promote compensatory growth of rats. However, lower expression of genes related to nutrient absorption and undifferentiated villous height (VH) were observed in the jejunum of rats in the LN group on day 70. In contrast, rats in the L group had lower VH on day 28 and day 70, while the expression of absorptive genes increased on day 28 compared to rats in the N group. Additionally, dramatic microbial changes in the jejunum of compensatory‐growth rats were observed, principally for Lactobacillus, Streptococcus, Corynebacterium and Staphylococcus. Moreover, the abundance of Lactobacillus, Streptococcus, Corynebacterium and Staphylococcus significantly correlated with gene expression in the jejunum as revealed by the correlation analysis. John Wiley and Sons Inc. 2018-04-06 /pmc/articles/PMC6033190/ /pubmed/29633535 http://dx.doi.org/10.1111/1751-7915.13266 Text en © 2018 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Zhu, Yizhi
Shi, Chao
Niu, Qingyan
Wang, Jing
Zhu, Weiyun
Dynamic changes in morphology, gene expression and microbiome in the jejunum of compensatory‐growth rats induced by protein restriction
title Dynamic changes in morphology, gene expression and microbiome in the jejunum of compensatory‐growth rats induced by protein restriction
title_full Dynamic changes in morphology, gene expression and microbiome in the jejunum of compensatory‐growth rats induced by protein restriction
title_fullStr Dynamic changes in morphology, gene expression and microbiome in the jejunum of compensatory‐growth rats induced by protein restriction
title_full_unstemmed Dynamic changes in morphology, gene expression and microbiome in the jejunum of compensatory‐growth rats induced by protein restriction
title_short Dynamic changes in morphology, gene expression and microbiome in the jejunum of compensatory‐growth rats induced by protein restriction
title_sort dynamic changes in morphology, gene expression and microbiome in the jejunum of compensatory‐growth rats induced by protein restriction
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6033190/
https://www.ncbi.nlm.nih.gov/pubmed/29633535
http://dx.doi.org/10.1111/1751-7915.13266
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