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Maternal diet during pregnancy is related with the infant stool microbiome in a delivery mode-dependent manner

BACKGROUND: The gut microbiome has an important role in infant health and immune development and may be affected by early-life exposures. Maternal diet may influence the infant gut microbiome through vertical transfer of maternal microbes to infants during vaginal delivery and breastfeeding. We aime...

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Autores principales: Lundgren, Sara N., Madan, Juliette C., Emond, Jennifer A., Morrison, Hilary G., Christensen, Brock C., Karagas, Margaret R., Hoen, Anne G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6033232/
https://www.ncbi.nlm.nih.gov/pubmed/29973274
http://dx.doi.org/10.1186/s40168-018-0490-8
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author Lundgren, Sara N.
Madan, Juliette C.
Emond, Jennifer A.
Morrison, Hilary G.
Christensen, Brock C.
Karagas, Margaret R.
Hoen, Anne G.
author_facet Lundgren, Sara N.
Madan, Juliette C.
Emond, Jennifer A.
Morrison, Hilary G.
Christensen, Brock C.
Karagas, Margaret R.
Hoen, Anne G.
author_sort Lundgren, Sara N.
collection PubMed
description BACKGROUND: The gut microbiome has an important role in infant health and immune development and may be affected by early-life exposures. Maternal diet may influence the infant gut microbiome through vertical transfer of maternal microbes to infants during vaginal delivery and breastfeeding. We aimed to examine the association of maternal diet during pregnancy with the infant gut microbiome 6 weeks post-delivery in mother-infant dyads enrolled in the New Hampshire Birth Cohort Study. Infant stool samples were collected from 145 infants, and maternal prenatal diet was assessed using a food frequency questionnaire. We used targeted sequencing of the 16S rRNA V4-V5 hypervariable region to characterize infant gut microbiota. To account for differences in baseline and trajectories of infant gut microbial profiles, we stratified analyses by delivery mode. RESULTS: We identified three infant gut microbiome clusters, characterized by increased abundance of Bifidobacterium, Streptococcus and Clostridium, and Bacteroides, respectively, overall and in the vaginally delivered infant stratum. In the analyses stratified to infants born vaginally and adjusted for other potential confounders, maternal fruit intake was associated with infant gut microbial community structure (PERMANOVA, p < 0.05). In multinomial logistic regression analyses, increased fruit intake was associated with an increased odds of belonging to the high Streptococcus/Clostridium group among infants born vaginally (OR (95% CI) = 2.73 (1.36, 5.46)). In infants delivered by Cesarean section, we identified three clusters that differed slightly from vaginally delivered infants, which were characterized by a high abundance of Bifidobacterium, high Clostridium and low Streptococcus and Ruminococcus genera, and high abundance of the family Enterobacteriaceae. Maternal dairy intake was associated with an increased odds of infants belonging to the high Clostridium cluster in infants born by Cesarean section (OR (95% CI) = 2.36 (1.05, 5.30)). Linear models suggested additional associations between maternal diet and infant intestinal microbes in both delivery mode strata. CONCLUSIONS: Our data indicate that maternal diet influences the infant gut microbiome and that these effects differ by delivery mode. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s40168-018-0490-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-60332322018-07-12 Maternal diet during pregnancy is related with the infant stool microbiome in a delivery mode-dependent manner Lundgren, Sara N. Madan, Juliette C. Emond, Jennifer A. Morrison, Hilary G. Christensen, Brock C. Karagas, Margaret R. Hoen, Anne G. Microbiome Research BACKGROUND: The gut microbiome has an important role in infant health and immune development and may be affected by early-life exposures. Maternal diet may influence the infant gut microbiome through vertical transfer of maternal microbes to infants during vaginal delivery and breastfeeding. We aimed to examine the association of maternal diet during pregnancy with the infant gut microbiome 6 weeks post-delivery in mother-infant dyads enrolled in the New Hampshire Birth Cohort Study. Infant stool samples were collected from 145 infants, and maternal prenatal diet was assessed using a food frequency questionnaire. We used targeted sequencing of the 16S rRNA V4-V5 hypervariable region to characterize infant gut microbiota. To account for differences in baseline and trajectories of infant gut microbial profiles, we stratified analyses by delivery mode. RESULTS: We identified three infant gut microbiome clusters, characterized by increased abundance of Bifidobacterium, Streptococcus and Clostridium, and Bacteroides, respectively, overall and in the vaginally delivered infant stratum. In the analyses stratified to infants born vaginally and adjusted for other potential confounders, maternal fruit intake was associated with infant gut microbial community structure (PERMANOVA, p < 0.05). In multinomial logistic regression analyses, increased fruit intake was associated with an increased odds of belonging to the high Streptococcus/Clostridium group among infants born vaginally (OR (95% CI) = 2.73 (1.36, 5.46)). In infants delivered by Cesarean section, we identified three clusters that differed slightly from vaginally delivered infants, which were characterized by a high abundance of Bifidobacterium, high Clostridium and low Streptococcus and Ruminococcus genera, and high abundance of the family Enterobacteriaceae. Maternal dairy intake was associated with an increased odds of infants belonging to the high Clostridium cluster in infants born by Cesarean section (OR (95% CI) = 2.36 (1.05, 5.30)). Linear models suggested additional associations between maternal diet and infant intestinal microbes in both delivery mode strata. CONCLUSIONS: Our data indicate that maternal diet influences the infant gut microbiome and that these effects differ by delivery mode. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s40168-018-0490-8) contains supplementary material, which is available to authorized users. BioMed Central 2018-07-05 /pmc/articles/PMC6033232/ /pubmed/29973274 http://dx.doi.org/10.1186/s40168-018-0490-8 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Lundgren, Sara N.
Madan, Juliette C.
Emond, Jennifer A.
Morrison, Hilary G.
Christensen, Brock C.
Karagas, Margaret R.
Hoen, Anne G.
Maternal diet during pregnancy is related with the infant stool microbiome in a delivery mode-dependent manner
title Maternal diet during pregnancy is related with the infant stool microbiome in a delivery mode-dependent manner
title_full Maternal diet during pregnancy is related with the infant stool microbiome in a delivery mode-dependent manner
title_fullStr Maternal diet during pregnancy is related with the infant stool microbiome in a delivery mode-dependent manner
title_full_unstemmed Maternal diet during pregnancy is related with the infant stool microbiome in a delivery mode-dependent manner
title_short Maternal diet during pregnancy is related with the infant stool microbiome in a delivery mode-dependent manner
title_sort maternal diet during pregnancy is related with the infant stool microbiome in a delivery mode-dependent manner
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6033232/
https://www.ncbi.nlm.nih.gov/pubmed/29973274
http://dx.doi.org/10.1186/s40168-018-0490-8
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