Cargando…
Synthetic antigen-binding fragments (Fabs) against S. mutans and S. sobrinus inhibit caries formation
Streptococcus mutans and Streptococcus sobrinus are the main causative agents of human dental caries. Current strategies for treating caries are costly and do not completely eradicate them completely. Passive immunization using nonhuman antibodies against Streptococcal surface antigens has shown suc...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6033933/ https://www.ncbi.nlm.nih.gov/pubmed/29976956 http://dx.doi.org/10.1038/s41598-018-28240-0 |
_version_ | 1783337773169639424 |
---|---|
author | Alam, Md. Kausar Zheng, Li Liu, Ruirui Papagerakis, Silvana Papagerakis, Petros Geyer, C. Ronald |
author_facet | Alam, Md. Kausar Zheng, Li Liu, Ruirui Papagerakis, Silvana Papagerakis, Petros Geyer, C. Ronald |
author_sort | Alam, Md. Kausar |
collection | PubMed |
description | Streptococcus mutans and Streptococcus sobrinus are the main causative agents of human dental caries. Current strategies for treating caries are costly and do not completely eradicate them completely. Passive immunization using nonhuman antibodies against Streptococcal surface antigens has shown success in human trials, however they often invoke immune reactions. We used phage display to generate human antigen-binding fragments (Fabs) against S. mutans and S. sobrinus. These Fabs were readily expressed in E. coli and bound to the surface S. mutans and S. sobrinus. Fabs inhibited sucrose-induced S. mutans and S. sobrinus biofilm formation in vitro and a combination of S. mutans and S. sobrinus Fabs prevented dental caries formation in a rat caries model. These results demonstrated that S. mutans and S. sobrinus Fabs could be used in passive immunization strategies to prevent dental caries. In the future, this strategy may be applied towards a caries therapy, whereby Fabs are topically applied to the tooth surface. |
format | Online Article Text |
id | pubmed-6033933 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-60339332018-07-12 Synthetic antigen-binding fragments (Fabs) against S. mutans and S. sobrinus inhibit caries formation Alam, Md. Kausar Zheng, Li Liu, Ruirui Papagerakis, Silvana Papagerakis, Petros Geyer, C. Ronald Sci Rep Article Streptococcus mutans and Streptococcus sobrinus are the main causative agents of human dental caries. Current strategies for treating caries are costly and do not completely eradicate them completely. Passive immunization using nonhuman antibodies against Streptococcal surface antigens has shown success in human trials, however they often invoke immune reactions. We used phage display to generate human antigen-binding fragments (Fabs) against S. mutans and S. sobrinus. These Fabs were readily expressed in E. coli and bound to the surface S. mutans and S. sobrinus. Fabs inhibited sucrose-induced S. mutans and S. sobrinus biofilm formation in vitro and a combination of S. mutans and S. sobrinus Fabs prevented dental caries formation in a rat caries model. These results demonstrated that S. mutans and S. sobrinus Fabs could be used in passive immunization strategies to prevent dental caries. In the future, this strategy may be applied towards a caries therapy, whereby Fabs are topically applied to the tooth surface. Nature Publishing Group UK 2018-07-05 /pmc/articles/PMC6033933/ /pubmed/29976956 http://dx.doi.org/10.1038/s41598-018-28240-0 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Alam, Md. Kausar Zheng, Li Liu, Ruirui Papagerakis, Silvana Papagerakis, Petros Geyer, C. Ronald Synthetic antigen-binding fragments (Fabs) against S. mutans and S. sobrinus inhibit caries formation |
title | Synthetic antigen-binding fragments (Fabs) against S. mutans and S. sobrinus inhibit caries formation |
title_full | Synthetic antigen-binding fragments (Fabs) against S. mutans and S. sobrinus inhibit caries formation |
title_fullStr | Synthetic antigen-binding fragments (Fabs) against S. mutans and S. sobrinus inhibit caries formation |
title_full_unstemmed | Synthetic antigen-binding fragments (Fabs) against S. mutans and S. sobrinus inhibit caries formation |
title_short | Synthetic antigen-binding fragments (Fabs) against S. mutans and S. sobrinus inhibit caries formation |
title_sort | synthetic antigen-binding fragments (fabs) against s. mutans and s. sobrinus inhibit caries formation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6033933/ https://www.ncbi.nlm.nih.gov/pubmed/29976956 http://dx.doi.org/10.1038/s41598-018-28240-0 |
work_keys_str_mv | AT alammdkausar syntheticantigenbindingfragmentsfabsagainstsmutansandssobrinusinhibitcariesformation AT zhengli syntheticantigenbindingfragmentsfabsagainstsmutansandssobrinusinhibitcariesformation AT liuruirui syntheticantigenbindingfragmentsfabsagainstsmutansandssobrinusinhibitcariesformation AT papagerakissilvana syntheticantigenbindingfragmentsfabsagainstsmutansandssobrinusinhibitcariesformation AT papagerakispetros syntheticantigenbindingfragmentsfabsagainstsmutansandssobrinusinhibitcariesformation AT geyercronald syntheticantigenbindingfragmentsfabsagainstsmutansandssobrinusinhibitcariesformation |