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Metagenomic analysis of basal ice from an Alaskan glacier
BACKGROUND: Glaciers cover ~ 10% of land but are among the least explored environments on Earth. The basal portion of glaciers often harbors unique aquatic microbial ecosystems in the absence of sunlight, and knowledge on the microbial community structures and their metabolic potential is very limit...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6034282/ https://www.ncbi.nlm.nih.gov/pubmed/29976249 http://dx.doi.org/10.1186/s40168-018-0505-5 |
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author | Kayani, Masood ur Rehman Doyle, Shawn M. Sangwan, Naseer Wang, Guanqun Gilbert, Jack A. Christner, Brent C. Zhu, Ting F. |
author_facet | Kayani, Masood ur Rehman Doyle, Shawn M. Sangwan, Naseer Wang, Guanqun Gilbert, Jack A. Christner, Brent C. Zhu, Ting F. |
author_sort | Kayani, Masood ur Rehman |
collection | PubMed |
description | BACKGROUND: Glaciers cover ~ 10% of land but are among the least explored environments on Earth. The basal portion of glaciers often harbors unique aquatic microbial ecosystems in the absence of sunlight, and knowledge on the microbial community structures and their metabolic potential is very limited. Here, we provide insights into the microbial lifestyle present at the base of the Matanuska Glacier, Alaska. RESULTS: DNA and RNA were extracted from samples of the Matanuska Glacier basal ice. Using Illumina MiSeq and HiSeq sequencing, we investigated the microbial diversity with the metagenomic shotgun reads and 16S ribosomal RNA data. We further assembled 9 partial and draft bacterial genomes from the metagenomic assembly, and identified key metabolic pathways such as sulfur oxidation and nitrification. Collectively, our analyses suggest a prevalence of lithotrophic and heterotrophic metabolisms in the subglacial microbiome. CONCLUSION: Our results present the first metagenomic assembly and bacterial draft genomes for a subglacial environment. These results extend our understanding of the chemical and biological processes in subglacial environments critically influenced by global climate change. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s40168-018-0505-5) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6034282 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-60342822018-07-12 Metagenomic analysis of basal ice from an Alaskan glacier Kayani, Masood ur Rehman Doyle, Shawn M. Sangwan, Naseer Wang, Guanqun Gilbert, Jack A. Christner, Brent C. Zhu, Ting F. Microbiome Microbiome Announcement BACKGROUND: Glaciers cover ~ 10% of land but are among the least explored environments on Earth. The basal portion of glaciers often harbors unique aquatic microbial ecosystems in the absence of sunlight, and knowledge on the microbial community structures and their metabolic potential is very limited. Here, we provide insights into the microbial lifestyle present at the base of the Matanuska Glacier, Alaska. RESULTS: DNA and RNA were extracted from samples of the Matanuska Glacier basal ice. Using Illumina MiSeq and HiSeq sequencing, we investigated the microbial diversity with the metagenomic shotgun reads and 16S ribosomal RNA data. We further assembled 9 partial and draft bacterial genomes from the metagenomic assembly, and identified key metabolic pathways such as sulfur oxidation and nitrification. Collectively, our analyses suggest a prevalence of lithotrophic and heterotrophic metabolisms in the subglacial microbiome. CONCLUSION: Our results present the first metagenomic assembly and bacterial draft genomes for a subglacial environment. These results extend our understanding of the chemical and biological processes in subglacial environments critically influenced by global climate change. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s40168-018-0505-5) contains supplementary material, which is available to authorized users. BioMed Central 2018-07-05 /pmc/articles/PMC6034282/ /pubmed/29976249 http://dx.doi.org/10.1186/s40168-018-0505-5 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Microbiome Announcement Kayani, Masood ur Rehman Doyle, Shawn M. Sangwan, Naseer Wang, Guanqun Gilbert, Jack A. Christner, Brent C. Zhu, Ting F. Metagenomic analysis of basal ice from an Alaskan glacier |
title | Metagenomic analysis of basal ice from an Alaskan glacier |
title_full | Metagenomic analysis of basal ice from an Alaskan glacier |
title_fullStr | Metagenomic analysis of basal ice from an Alaskan glacier |
title_full_unstemmed | Metagenomic analysis of basal ice from an Alaskan glacier |
title_short | Metagenomic analysis of basal ice from an Alaskan glacier |
title_sort | metagenomic analysis of basal ice from an alaskan glacier |
topic | Microbiome Announcement |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6034282/ https://www.ncbi.nlm.nih.gov/pubmed/29976249 http://dx.doi.org/10.1186/s40168-018-0505-5 |
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