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Honey reduces the metastatic characteristics of prostate cancer cell lines by promoting a loss of adhesion

Honey has been shown to have a range of therapeutic effects in humans, with anti-inflammatory and anti-bacterial effects among those previously characterised. Here, we examine the possibility of New Zealand thyme, manuka and honeydew honeys, and their major sugar and phenolic components, reducing th...

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Autores principales: Abel, Sean D.A., Dadhwal, Sumit, Gamble, Allan B., Baird, Sarah K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6034594/
https://www.ncbi.nlm.nih.gov/pubmed/30002964
http://dx.doi.org/10.7717/peerj.5115
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author Abel, Sean D.A.
Dadhwal, Sumit
Gamble, Allan B.
Baird, Sarah K.
author_facet Abel, Sean D.A.
Dadhwal, Sumit
Gamble, Allan B.
Baird, Sarah K.
author_sort Abel, Sean D.A.
collection PubMed
description Honey has been shown to have a range of therapeutic effects in humans, with anti-inflammatory and anti-bacterial effects among those previously characterised. Here, we examine the possibility of New Zealand thyme, manuka and honeydew honeys, and their major sugar and phenolic components, reducing the development of metastatic cancer. Their activity was examined in vitro, in PC3 and DU145 prostate cancer cell lines, through measuring the compounds’ effects on the metastatic characteristics of migration, invasion and adhesion. First, the phenolic compounds gallic acid, caffeic acid, quercetin, kaempferol and chrysin were quantified in the honeys using high performance liquid chromatography, and found in nanomolar concentrations. In a Boyden chamber-based migration assay, non-toxic concentrations of thyme and honeydew honeys reduced cell migration by 20%, and all phenolic compounds except caffeic acid also lowered migration, although a mixture of only the sugars found in honey had no effect. All of the honeys, phenolics and the sugar-only mixture reduced invasive movement of cells through extracellular matrix by up to 75%. Most notably, each of the three honeys and the sugar-only mixture reduced cell adhesion to collagen I by 90%. With the exception of quercetin, phenolic compounds did not reduce adhesion. Therefore, honey and its sugar and phenolic components can lower the metastatic properties of cancer cells, and may do this by preventing effective cell adhesion to the extracellular matrix. The sugars and phenol compounds of honey are much more effective in combination than individually.
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spelling pubmed-60345942018-07-12 Honey reduces the metastatic characteristics of prostate cancer cell lines by promoting a loss of adhesion Abel, Sean D.A. Dadhwal, Sumit Gamble, Allan B. Baird, Sarah K. PeerJ Cell Biology Honey has been shown to have a range of therapeutic effects in humans, with anti-inflammatory and anti-bacterial effects among those previously characterised. Here, we examine the possibility of New Zealand thyme, manuka and honeydew honeys, and their major sugar and phenolic components, reducing the development of metastatic cancer. Their activity was examined in vitro, in PC3 and DU145 prostate cancer cell lines, through measuring the compounds’ effects on the metastatic characteristics of migration, invasion and adhesion. First, the phenolic compounds gallic acid, caffeic acid, quercetin, kaempferol and chrysin were quantified in the honeys using high performance liquid chromatography, and found in nanomolar concentrations. In a Boyden chamber-based migration assay, non-toxic concentrations of thyme and honeydew honeys reduced cell migration by 20%, and all phenolic compounds except caffeic acid also lowered migration, although a mixture of only the sugars found in honey had no effect. All of the honeys, phenolics and the sugar-only mixture reduced invasive movement of cells through extracellular matrix by up to 75%. Most notably, each of the three honeys and the sugar-only mixture reduced cell adhesion to collagen I by 90%. With the exception of quercetin, phenolic compounds did not reduce adhesion. Therefore, honey and its sugar and phenolic components can lower the metastatic properties of cancer cells, and may do this by preventing effective cell adhesion to the extracellular matrix. The sugars and phenol compounds of honey are much more effective in combination than individually. PeerJ Inc. 2018-07-03 /pmc/articles/PMC6034594/ /pubmed/30002964 http://dx.doi.org/10.7717/peerj.5115 Text en ©2018 Abel et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Cell Biology
Abel, Sean D.A.
Dadhwal, Sumit
Gamble, Allan B.
Baird, Sarah K.
Honey reduces the metastatic characteristics of prostate cancer cell lines by promoting a loss of adhesion
title Honey reduces the metastatic characteristics of prostate cancer cell lines by promoting a loss of adhesion
title_full Honey reduces the metastatic characteristics of prostate cancer cell lines by promoting a loss of adhesion
title_fullStr Honey reduces the metastatic characteristics of prostate cancer cell lines by promoting a loss of adhesion
title_full_unstemmed Honey reduces the metastatic characteristics of prostate cancer cell lines by promoting a loss of adhesion
title_short Honey reduces the metastatic characteristics of prostate cancer cell lines by promoting a loss of adhesion
title_sort honey reduces the metastatic characteristics of prostate cancer cell lines by promoting a loss of adhesion
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6034594/
https://www.ncbi.nlm.nih.gov/pubmed/30002964
http://dx.doi.org/10.7717/peerj.5115
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