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Estrous cycle-dependent modulation of in vivo microvascular dysfunction after nanomaterial inhalation

Preconceptive health encompasses male and female reproductive capability. In females, this takes into account each of the stages of the estrous cycle. Microvascular reactivity varies throughout the estrous cycle in response to hormonal changes and in preparation for pregnancy. Microvascular alterati...

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Autores principales: Stapleton, P.A., McBride, C.R., Yi, J., Abukabda, A.B., Nurkiewicz, T.R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6034709/
https://www.ncbi.nlm.nih.gov/pubmed/29545171
http://dx.doi.org/10.1016/j.reprotox.2018.03.001
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author Stapleton, P.A.
McBride, C.R.
Yi, J.
Abukabda, A.B.
Nurkiewicz, T.R.
author_facet Stapleton, P.A.
McBride, C.R.
Yi, J.
Abukabda, A.B.
Nurkiewicz, T.R.
author_sort Stapleton, P.A.
collection PubMed
description Preconceptive health encompasses male and female reproductive capability. In females, this takes into account each of the stages of the estrous cycle. Microvascular reactivity varies throughout the estrous cycle in response to hormonal changes and in preparation for pregnancy. Microvascular alterations in response to engineered nanomaterial (ENM) exposure have been described within 24-h of inhalation; however, the impact upon the uterine vasculature at differing estrous stages and at late-stage pregnancy is unclear. Female Sprague Dawley (SD) rats (virgin and late stage pregnancy [GD 19]) were exposed to nano-TiO aerosols (173.2 ± 6.4 nm, 10.2 ± 0.46 mg/m(3), 5 h) 24-h prior to experimentation leading to a single calculated deposition of 42.2 ± 1.9 µg nano- TiO(2) (exposed) or 0µg (control). Animals were anesthetized, estrous status verified, and prepared for in situ assessment of leukocyte trafficking and vascular function by means of intravital microscopy, Uterine basal arteriolar reactivity was stimulated using iontophoretically applied chemicals: acetylcholine (ACh, 0.025 M; 20, 40, 100, 200 nA), sodium nitroprusside (SNP, 0.05 M; 20, 40, 100 nA), phenylephrine (PE, 0.05 M; 20, 40, 100 nA). Finally, adenosine (ADO, 10(−4) M) was superfused over the tissue to identify maximum diameter. In situ vessel reactivity after exposure was significantly blunted based on estrous stage, but not at late-stage pregnancy. Local uterine venular leukocyte trafficking and systemic inflammatory markers were also significantly affected during preparatory (proestrus), fertile (estrus), and infertile (diestrus) periods after ENM inhalation. Overall, these deficits in reactivity and increased inflammatory activity may impair female fertility after ENM exposure.
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spelling pubmed-60347092018-07-06 Estrous cycle-dependent modulation of in vivo microvascular dysfunction after nanomaterial inhalation Stapleton, P.A. McBride, C.R. Yi, J. Abukabda, A.B. Nurkiewicz, T.R. Reprod Toxicol Article Preconceptive health encompasses male and female reproductive capability. In females, this takes into account each of the stages of the estrous cycle. Microvascular reactivity varies throughout the estrous cycle in response to hormonal changes and in preparation for pregnancy. Microvascular alterations in response to engineered nanomaterial (ENM) exposure have been described within 24-h of inhalation; however, the impact upon the uterine vasculature at differing estrous stages and at late-stage pregnancy is unclear. Female Sprague Dawley (SD) rats (virgin and late stage pregnancy [GD 19]) were exposed to nano-TiO aerosols (173.2 ± 6.4 nm, 10.2 ± 0.46 mg/m(3), 5 h) 24-h prior to experimentation leading to a single calculated deposition of 42.2 ± 1.9 µg nano- TiO(2) (exposed) or 0µg (control). Animals were anesthetized, estrous status verified, and prepared for in situ assessment of leukocyte trafficking and vascular function by means of intravital microscopy, Uterine basal arteriolar reactivity was stimulated using iontophoretically applied chemicals: acetylcholine (ACh, 0.025 M; 20, 40, 100, 200 nA), sodium nitroprusside (SNP, 0.05 M; 20, 40, 100 nA), phenylephrine (PE, 0.05 M; 20, 40, 100 nA). Finally, adenosine (ADO, 10(−4) M) was superfused over the tissue to identify maximum diameter. In situ vessel reactivity after exposure was significantly blunted based on estrous stage, but not at late-stage pregnancy. Local uterine venular leukocyte trafficking and systemic inflammatory markers were also significantly affected during preparatory (proestrus), fertile (estrus), and infertile (diestrus) periods after ENM inhalation. Overall, these deficits in reactivity and increased inflammatory activity may impair female fertility after ENM exposure. 2018-03-12 2018-06 /pmc/articles/PMC6034709/ /pubmed/29545171 http://dx.doi.org/10.1016/j.reprotox.2018.03.001 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Stapleton, P.A.
McBride, C.R.
Yi, J.
Abukabda, A.B.
Nurkiewicz, T.R.
Estrous cycle-dependent modulation of in vivo microvascular dysfunction after nanomaterial inhalation
title Estrous cycle-dependent modulation of in vivo microvascular dysfunction after nanomaterial inhalation
title_full Estrous cycle-dependent modulation of in vivo microvascular dysfunction after nanomaterial inhalation
title_fullStr Estrous cycle-dependent modulation of in vivo microvascular dysfunction after nanomaterial inhalation
title_full_unstemmed Estrous cycle-dependent modulation of in vivo microvascular dysfunction after nanomaterial inhalation
title_short Estrous cycle-dependent modulation of in vivo microvascular dysfunction after nanomaterial inhalation
title_sort estrous cycle-dependent modulation of in vivo microvascular dysfunction after nanomaterial inhalation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6034709/
https://www.ncbi.nlm.nih.gov/pubmed/29545171
http://dx.doi.org/10.1016/j.reprotox.2018.03.001
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