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Diagnostic Value of Methylated Septin9 for Colorectal Cancer Detection
BACKGROUND: Methylated Septin9 (mSEPT9) has been suggested as a reliable biomarker in colorectal cancer (CRC) detection. We aimed to determine the diagnostic value of mSEPT9 for CRC detection in Chinese patients. In addition, we compared the diagnostic efficacy of mSEPT9 to traditional screening met...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6036110/ https://www.ncbi.nlm.nih.gov/pubmed/30013949 http://dx.doi.org/10.3389/fonc.2018.00247 |
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author | Xie, Li Jiang, Xiyi Li, Qian Sun, Zujun Quan, Wenqiang Duan, Yuping Li, Dong Chen, Tianhui |
author_facet | Xie, Li Jiang, Xiyi Li, Qian Sun, Zujun Quan, Wenqiang Duan, Yuping Li, Dong Chen, Tianhui |
author_sort | Xie, Li |
collection | PubMed |
description | BACKGROUND: Methylated Septin9 (mSEPT9) has been suggested as a reliable biomarker in colorectal cancer (CRC) detection. We aimed to determine the diagnostic value of mSEPT9 for CRC detection in Chinese patients. In addition, we compared the diagnostic efficacy of mSEPT9 to traditional screening method [fecal occult blood test (FOBT)] and two biomarkers [carcinoembryonic antigen (CEA) and carbohydrate antigen-199 (Ca-199)]. METHODS: Overall 248 subjects including 123 patients with CRC and 125 controls were included. Plasma and fecal samples were collected for CEA, Ca-199, mSEPT9, and FOBT tests. Sensitivity and specificity were calculated to evaluate the diagnostic efficacy of each method; receiver operating characteristic (ROC) curve was plotted for the assessment of diagnostic accuracy, and comparisons among FOBT, mSEPT9, and the combination were assessed through area under the ROC curve (AUC). RESULTS: mSEPT9 achieved overall sensitivity and specificity of 61.8% [95% confidence interval (CI): 53.0–69.9%] and 89.6% (83.0–93.8%), respectively, with an AUC value of 0.757 (95% CI: 0.701–0.807), superior to FOBT [sensitivity: 61.4% (50.9–70.9%); specificity: 70.3% (59.1–79.5%); AUC: 0.658 (0.578–0.723)], CEA [sensitivity: 35.0% (27.1–43.7%); specificity: 62.6% (53.8–70.7%); AUC: 0.485 (0.411–0.559)], and Ca-199 [sensitivity: 17.9% (12.1–25.6%); specificity: 55.7% (48.9–64.1%); AUC: 0.353 (0.283–0.423)]. The combination of mSEPT9 and FOBT further improved sensitivity and AUC value of 84.1% (75.1–90.3%) and 0.807 (0.752–0.863), respectively, while specificity was declined to 62.2% (50.8–72.4%). CONCLUSION: mSEPT9 demonstrated best diagnostic ability in CRC detection compared with FOBT, CEA, and Ca-199. The combination of mSEPT9 and FOBT further improved diagnostic sensitivity especially for early stage disease, which may provide a new approach for future CRC screening, though further investigations are warranted. |
format | Online Article Text |
id | pubmed-6036110 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-60361102018-07-16 Diagnostic Value of Methylated Septin9 for Colorectal Cancer Detection Xie, Li Jiang, Xiyi Li, Qian Sun, Zujun Quan, Wenqiang Duan, Yuping Li, Dong Chen, Tianhui Front Oncol Oncology BACKGROUND: Methylated Septin9 (mSEPT9) has been suggested as a reliable biomarker in colorectal cancer (CRC) detection. We aimed to determine the diagnostic value of mSEPT9 for CRC detection in Chinese patients. In addition, we compared the diagnostic efficacy of mSEPT9 to traditional screening method [fecal occult blood test (FOBT)] and two biomarkers [carcinoembryonic antigen (CEA) and carbohydrate antigen-199 (Ca-199)]. METHODS: Overall 248 subjects including 123 patients with CRC and 125 controls were included. Plasma and fecal samples were collected for CEA, Ca-199, mSEPT9, and FOBT tests. Sensitivity and specificity were calculated to evaluate the diagnostic efficacy of each method; receiver operating characteristic (ROC) curve was plotted for the assessment of diagnostic accuracy, and comparisons among FOBT, mSEPT9, and the combination were assessed through area under the ROC curve (AUC). RESULTS: mSEPT9 achieved overall sensitivity and specificity of 61.8% [95% confidence interval (CI): 53.0–69.9%] and 89.6% (83.0–93.8%), respectively, with an AUC value of 0.757 (95% CI: 0.701–0.807), superior to FOBT [sensitivity: 61.4% (50.9–70.9%); specificity: 70.3% (59.1–79.5%); AUC: 0.658 (0.578–0.723)], CEA [sensitivity: 35.0% (27.1–43.7%); specificity: 62.6% (53.8–70.7%); AUC: 0.485 (0.411–0.559)], and Ca-199 [sensitivity: 17.9% (12.1–25.6%); specificity: 55.7% (48.9–64.1%); AUC: 0.353 (0.283–0.423)]. The combination of mSEPT9 and FOBT further improved sensitivity and AUC value of 84.1% (75.1–90.3%) and 0.807 (0.752–0.863), respectively, while specificity was declined to 62.2% (50.8–72.4%). CONCLUSION: mSEPT9 demonstrated best diagnostic ability in CRC detection compared with FOBT, CEA, and Ca-199. The combination of mSEPT9 and FOBT further improved diagnostic sensitivity especially for early stage disease, which may provide a new approach for future CRC screening, though further investigations are warranted. Frontiers Media S.A. 2018-07-02 /pmc/articles/PMC6036110/ /pubmed/30013949 http://dx.doi.org/10.3389/fonc.2018.00247 Text en Copyright © 2018 Xie, Jiang, Li, Sun, Quan, Duan, Li and Chen. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Oncology Xie, Li Jiang, Xiyi Li, Qian Sun, Zujun Quan, Wenqiang Duan, Yuping Li, Dong Chen, Tianhui Diagnostic Value of Methylated Septin9 for Colorectal Cancer Detection |
title | Diagnostic Value of Methylated Septin9 for Colorectal Cancer Detection |
title_full | Diagnostic Value of Methylated Septin9 for Colorectal Cancer Detection |
title_fullStr | Diagnostic Value of Methylated Septin9 for Colorectal Cancer Detection |
title_full_unstemmed | Diagnostic Value of Methylated Septin9 for Colorectal Cancer Detection |
title_short | Diagnostic Value of Methylated Septin9 for Colorectal Cancer Detection |
title_sort | diagnostic value of methylated septin9 for colorectal cancer detection |
topic | Oncology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6036110/ https://www.ncbi.nlm.nih.gov/pubmed/30013949 http://dx.doi.org/10.3389/fonc.2018.00247 |
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