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Effect of Early Weaning on the Intestinal Microbiota and Expression of Genes Related to Barrier Function in Lambs
Weaning stress has been reported to impair intestinal health. The gut microbiota plays a vital role in the long-term health of the host. However, our understanding of weaning stress on gut microbiota and barrier function is very limited in livestock species, especially lambs. We investigated the eff...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6036172/ https://www.ncbi.nlm.nih.gov/pubmed/30013534 http://dx.doi.org/10.3389/fmicb.2018.01431 |
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author | Li, Chong Wang, Weimin Liu, Ting Zhang, Qian Wang, Guoxiu Li, Fadi Li, Fei Yue, Xiangpeng Li, Tingfu |
author_facet | Li, Chong Wang, Weimin Liu, Ting Zhang, Qian Wang, Guoxiu Li, Fadi Li, Fei Yue, Xiangpeng Li, Tingfu |
author_sort | Li, Chong |
collection | PubMed |
description | Weaning stress has been reported to impair intestinal health. The gut microbiota plays a vital role in the long-term health of the host. However, our understanding of weaning stress on gut microbiota and barrier function is very limited in livestock species, especially lambs. We investigated the effects of early weaning stress on intestinal bacterial communities and intestinal barrier function in lambs. A total of 24 neonatal male Hu lambs were randomly allocated into two groups, one weaned on day 28 and the other weaned on day 56. At 42 and 84 days, six lambs from each group were randomly selected and sacrificed. Ileal tissue and ileal digesta were collected to compare the differences in ileal microbiota and the mRNA levels of Toll-like receptors (TLRs) and tight junction proteins between the early weaning group and the control group at day 42 when the early weaning group have been weaned for 14 days, and at day 84 when the 28 and 56 days weaning groups had been weaned for 56 and 28 days, respectively. 16S rRNA gene sequencing of ileal samples revealed that the ileal microbiota was very different between the two groups, even at 84 days of age. Early weaning significantly increased alpha diversity and altered the relative abundance of several bacterial taxa. The expression of genes related to intestinal barrier function was affected by early weaning. Early weaning significantly increased ileal mRNA levels of TLR1 on days 42 and 84; TLR2, TLR4, and TLR5 on day 84; claudin1 and claudin4 on day 42; and occludin on day 84. We demonstrate that early weaning not only altered the ileal microbiota on day 42 (compared with lambs that were not weaned), but also had lasting effects on the ileal microbiota at day 84; furthermore, early weaning impacts expression levels of genes related to intestinal barrier function. |
format | Online Article Text |
id | pubmed-6036172 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-60361722018-07-16 Effect of Early Weaning on the Intestinal Microbiota and Expression of Genes Related to Barrier Function in Lambs Li, Chong Wang, Weimin Liu, Ting Zhang, Qian Wang, Guoxiu Li, Fadi Li, Fei Yue, Xiangpeng Li, Tingfu Front Microbiol Microbiology Weaning stress has been reported to impair intestinal health. The gut microbiota plays a vital role in the long-term health of the host. However, our understanding of weaning stress on gut microbiota and barrier function is very limited in livestock species, especially lambs. We investigated the effects of early weaning stress on intestinal bacterial communities and intestinal barrier function in lambs. A total of 24 neonatal male Hu lambs were randomly allocated into two groups, one weaned on day 28 and the other weaned on day 56. At 42 and 84 days, six lambs from each group were randomly selected and sacrificed. Ileal tissue and ileal digesta were collected to compare the differences in ileal microbiota and the mRNA levels of Toll-like receptors (TLRs) and tight junction proteins between the early weaning group and the control group at day 42 when the early weaning group have been weaned for 14 days, and at day 84 when the 28 and 56 days weaning groups had been weaned for 56 and 28 days, respectively. 16S rRNA gene sequencing of ileal samples revealed that the ileal microbiota was very different between the two groups, even at 84 days of age. Early weaning significantly increased alpha diversity and altered the relative abundance of several bacterial taxa. The expression of genes related to intestinal barrier function was affected by early weaning. Early weaning significantly increased ileal mRNA levels of TLR1 on days 42 and 84; TLR2, TLR4, and TLR5 on day 84; claudin1 and claudin4 on day 42; and occludin on day 84. We demonstrate that early weaning not only altered the ileal microbiota on day 42 (compared with lambs that were not weaned), but also had lasting effects on the ileal microbiota at day 84; furthermore, early weaning impacts expression levels of genes related to intestinal barrier function. Frontiers Media S.A. 2018-07-02 /pmc/articles/PMC6036172/ /pubmed/30013534 http://dx.doi.org/10.3389/fmicb.2018.01431 Text en Copyright © 2018 Li, Wang, Liu, Zhang, Wang, Li, Li, Yue and Li. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Li, Chong Wang, Weimin Liu, Ting Zhang, Qian Wang, Guoxiu Li, Fadi Li, Fei Yue, Xiangpeng Li, Tingfu Effect of Early Weaning on the Intestinal Microbiota and Expression of Genes Related to Barrier Function in Lambs |
title | Effect of Early Weaning on the Intestinal Microbiota and Expression of Genes Related to Barrier Function in Lambs |
title_full | Effect of Early Weaning on the Intestinal Microbiota and Expression of Genes Related to Barrier Function in Lambs |
title_fullStr | Effect of Early Weaning on the Intestinal Microbiota and Expression of Genes Related to Barrier Function in Lambs |
title_full_unstemmed | Effect of Early Weaning on the Intestinal Microbiota and Expression of Genes Related to Barrier Function in Lambs |
title_short | Effect of Early Weaning on the Intestinal Microbiota and Expression of Genes Related to Barrier Function in Lambs |
title_sort | effect of early weaning on the intestinal microbiota and expression of genes related to barrier function in lambs |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6036172/ https://www.ncbi.nlm.nih.gov/pubmed/30013534 http://dx.doi.org/10.3389/fmicb.2018.01431 |
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