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HHV-6 Specific T-Cell Immunity in Healthy Children and Adolescents
Objective: Primary infection with human herpes virus 6 (mainly HHV-6B) commonly occurs in the first 2 years of life leading to persistence and the possibility of virus reactivation later in life. Consequently, a specific cellular immune response is essential for effective control of virus reactivati...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6036236/ https://www.ncbi.nlm.nih.gov/pubmed/30013962 http://dx.doi.org/10.3389/fped.2018.00191 |
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author | Schwarz, Christine M. Strenger, Volker Strohmaier, Heimo Singer, Georg Kaiser, Margarita Raicht, Andrea Schwinger, Wolfgang Urban, Christian |
author_facet | Schwarz, Christine M. Strenger, Volker Strohmaier, Heimo Singer, Georg Kaiser, Margarita Raicht, Andrea Schwinger, Wolfgang Urban, Christian |
author_sort | Schwarz, Christine M. |
collection | PubMed |
description | Objective: Primary infection with human herpes virus 6 (mainly HHV-6B) commonly occurs in the first 2 years of life leading to persistence and the possibility of virus reactivation later in life. Consequently, a specific cellular immune response is essential for effective control of virus reactivation. We have studied cell-mediated immune response to HHV-6 (U54) in healthy children and adolescents. Materials and Methods: By flow cytometry, the amount of cytokine (interferon gamma—IFN- γ, interleukin 2—IL-2, tumor necrosis factor alpha—TNF-α) secreting T-cells were measured after 10 days of pre-sensitization and 6 h of re-stimulation with mixtures of pooled overlapping peptides from U54, staphylococcal enterotoxin B (SEB, positive control), or Actin (negative control) in healthy children and adolescents without any underlying immune disorder or infectious disease. Results: All individuals showed a virus-specific response for at least one cytokine in either CD4+ or CD8+ cells. Percentages of individuals with HHV-6-specific TNF-α response in CD4+ (48% of individuals) as well as CD8+ (56% of individuals) were always the highest. Our data show significantly higher frequencies of HHV-6-specific TNF-α producing CD8+ T-cells in individuals older than 10 years of life (p = 0.033). Additionally, the frequency of HHV-6 specific TNF-α producing CD8+ T-cells positively correlated with the age of the individuals. Linear regression analysis showed a positive relation between age and frequency of HHV-6-specific TNF-α producing CD8+ T-cells. Conclusion: Results indicate that T-cell immune response against HHV-6 is commonly detectable in healthy children and adolescents with higher frequencies of antigen-specific T-cells in older children and adolescents possibly reflecting repeated stimulation by viral persistence and subclinical reactivation. |
format | Online Article Text |
id | pubmed-6036236 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-60362362018-07-16 HHV-6 Specific T-Cell Immunity in Healthy Children and Adolescents Schwarz, Christine M. Strenger, Volker Strohmaier, Heimo Singer, Georg Kaiser, Margarita Raicht, Andrea Schwinger, Wolfgang Urban, Christian Front Pediatr Pediatrics Objective: Primary infection with human herpes virus 6 (mainly HHV-6B) commonly occurs in the first 2 years of life leading to persistence and the possibility of virus reactivation later in life. Consequently, a specific cellular immune response is essential for effective control of virus reactivation. We have studied cell-mediated immune response to HHV-6 (U54) in healthy children and adolescents. Materials and Methods: By flow cytometry, the amount of cytokine (interferon gamma—IFN- γ, interleukin 2—IL-2, tumor necrosis factor alpha—TNF-α) secreting T-cells were measured after 10 days of pre-sensitization and 6 h of re-stimulation with mixtures of pooled overlapping peptides from U54, staphylococcal enterotoxin B (SEB, positive control), or Actin (negative control) in healthy children and adolescents without any underlying immune disorder or infectious disease. Results: All individuals showed a virus-specific response for at least one cytokine in either CD4+ or CD8+ cells. Percentages of individuals with HHV-6-specific TNF-α response in CD4+ (48% of individuals) as well as CD8+ (56% of individuals) were always the highest. Our data show significantly higher frequencies of HHV-6-specific TNF-α producing CD8+ T-cells in individuals older than 10 years of life (p = 0.033). Additionally, the frequency of HHV-6 specific TNF-α producing CD8+ T-cells positively correlated with the age of the individuals. Linear regression analysis showed a positive relation between age and frequency of HHV-6-specific TNF-α producing CD8+ T-cells. Conclusion: Results indicate that T-cell immune response against HHV-6 is commonly detectable in healthy children and adolescents with higher frequencies of antigen-specific T-cells in older children and adolescents possibly reflecting repeated stimulation by viral persistence and subclinical reactivation. Frontiers Media S.A. 2018-07-02 /pmc/articles/PMC6036236/ /pubmed/30013962 http://dx.doi.org/10.3389/fped.2018.00191 Text en Copyright © 2018 Schwarz, Strenger, Strohmaier, Singer, Kaiser, Raicht, Schwinger and Urban. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pediatrics Schwarz, Christine M. Strenger, Volker Strohmaier, Heimo Singer, Georg Kaiser, Margarita Raicht, Andrea Schwinger, Wolfgang Urban, Christian HHV-6 Specific T-Cell Immunity in Healthy Children and Adolescents |
title | HHV-6 Specific T-Cell Immunity in Healthy Children and Adolescents |
title_full | HHV-6 Specific T-Cell Immunity in Healthy Children and Adolescents |
title_fullStr | HHV-6 Specific T-Cell Immunity in Healthy Children and Adolescents |
title_full_unstemmed | HHV-6 Specific T-Cell Immunity in Healthy Children and Adolescents |
title_short | HHV-6 Specific T-Cell Immunity in Healthy Children and Adolescents |
title_sort | hhv-6 specific t-cell immunity in healthy children and adolescents |
topic | Pediatrics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6036236/ https://www.ncbi.nlm.nih.gov/pubmed/30013962 http://dx.doi.org/10.3389/fped.2018.00191 |
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