Cargando…

Integrated Transcript and Metabolite Profiles Reveal That EbCHI Plays an Important Role in Scutellarin Accumulation in Erigeron breviscapus Hairy Roots

Scutellarin, a flavonoid 7-O-glucuronide, is an essential bioactive compound of Erigeron breviscapus (Vaniot) Hand.-Mazz. used for the treatment of cerebrovascular diseases. However, due to overexploitation and overuse, E. breviscapus is facing the problems of extinction and habitat degradation. In...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Ruibing, Chen, Xianghui, Zhu, Tingting, Liu, Jianghua, Xiang, Xing, Yu, Jian, Tan, Hexin, Gao, Shouhong, Li, Qing, Fang, Yichao, Chen, Wansheng, Zhang, Lei, Huang, Baokang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6036287/
https://www.ncbi.nlm.nih.gov/pubmed/30013578
http://dx.doi.org/10.3389/fpls.2018.00789
_version_ 1783338137200623616
author Chen, Ruibing
Chen, Xianghui
Zhu, Tingting
Liu, Jianghua
Xiang, Xing
Yu, Jian
Tan, Hexin
Gao, Shouhong
Li, Qing
Fang, Yichao
Chen, Wansheng
Zhang, Lei
Huang, Baokang
author_facet Chen, Ruibing
Chen, Xianghui
Zhu, Tingting
Liu, Jianghua
Xiang, Xing
Yu, Jian
Tan, Hexin
Gao, Shouhong
Li, Qing
Fang, Yichao
Chen, Wansheng
Zhang, Lei
Huang, Baokang
author_sort Chen, Ruibing
collection PubMed
description Scutellarin, a flavonoid 7-O-glucuronide, is an essential bioactive compound of Erigeron breviscapus (Vaniot) Hand.-Mazz. used for the treatment of cerebrovascular diseases. However, due to overexploitation and overuse, E. breviscapus is facing the problems of extinction and habitat degradation. In this study, a correlation analysis between the transcript and metabolite profiles of methyl jasmonate (MeJA)-treated E. breviscapus at different time points indicated that chalcone isomerase (EbCHI) was the primary contributor to scutellarin accumulation during flavonoid biosynthesis. EbCHI was then further characterized as a chalcone isomerase that efficiently converted chalcone to naringenin in vitro. Optimal parameters derived by comparing different culture conditions were successfully used to establish hairy root cultures of E. breviscapus with a maximum transformation rate of 60% in B5 medium. Furthermore, overexpression of EbCHI significantly enhanced scutellarin accumulation in E. breviscapus hairy roots with a maximum content of 2.21 mg g(-1) (dw), 10-fold higher than that of natural roots (0.21 mg g(-1) dw). This study sheds new light on a method of effective gene-based metabolic engineering by accurate and appropriate strategies and provides a protocol for hairy root cultures that accumulate high levels of scutellarin, providing a promising prospect for relieving the overexploitation and unavailability of E. breviscapus in the future.
format Online
Article
Text
id pubmed-6036287
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-60362872018-07-16 Integrated Transcript and Metabolite Profiles Reveal That EbCHI Plays an Important Role in Scutellarin Accumulation in Erigeron breviscapus Hairy Roots Chen, Ruibing Chen, Xianghui Zhu, Tingting Liu, Jianghua Xiang, Xing Yu, Jian Tan, Hexin Gao, Shouhong Li, Qing Fang, Yichao Chen, Wansheng Zhang, Lei Huang, Baokang Front Plant Sci Plant Science Scutellarin, a flavonoid 7-O-glucuronide, is an essential bioactive compound of Erigeron breviscapus (Vaniot) Hand.-Mazz. used for the treatment of cerebrovascular diseases. However, due to overexploitation and overuse, E. breviscapus is facing the problems of extinction and habitat degradation. In this study, a correlation analysis between the transcript and metabolite profiles of methyl jasmonate (MeJA)-treated E. breviscapus at different time points indicated that chalcone isomerase (EbCHI) was the primary contributor to scutellarin accumulation during flavonoid biosynthesis. EbCHI was then further characterized as a chalcone isomerase that efficiently converted chalcone to naringenin in vitro. Optimal parameters derived by comparing different culture conditions were successfully used to establish hairy root cultures of E. breviscapus with a maximum transformation rate of 60% in B5 medium. Furthermore, overexpression of EbCHI significantly enhanced scutellarin accumulation in E. breviscapus hairy roots with a maximum content of 2.21 mg g(-1) (dw), 10-fold higher than that of natural roots (0.21 mg g(-1) dw). This study sheds new light on a method of effective gene-based metabolic engineering by accurate and appropriate strategies and provides a protocol for hairy root cultures that accumulate high levels of scutellarin, providing a promising prospect for relieving the overexploitation and unavailability of E. breviscapus in the future. Frontiers Media S.A. 2018-06-21 /pmc/articles/PMC6036287/ /pubmed/30013578 http://dx.doi.org/10.3389/fpls.2018.00789 Text en Copyright © 2018 Chen, Chen, Zhu, Liu, Xiang, Yu, Tan, Gao, Li, Fang, Chen, Zhang and Huang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Chen, Ruibing
Chen, Xianghui
Zhu, Tingting
Liu, Jianghua
Xiang, Xing
Yu, Jian
Tan, Hexin
Gao, Shouhong
Li, Qing
Fang, Yichao
Chen, Wansheng
Zhang, Lei
Huang, Baokang
Integrated Transcript and Metabolite Profiles Reveal That EbCHI Plays an Important Role in Scutellarin Accumulation in Erigeron breviscapus Hairy Roots
title Integrated Transcript and Metabolite Profiles Reveal That EbCHI Plays an Important Role in Scutellarin Accumulation in Erigeron breviscapus Hairy Roots
title_full Integrated Transcript and Metabolite Profiles Reveal That EbCHI Plays an Important Role in Scutellarin Accumulation in Erigeron breviscapus Hairy Roots
title_fullStr Integrated Transcript and Metabolite Profiles Reveal That EbCHI Plays an Important Role in Scutellarin Accumulation in Erigeron breviscapus Hairy Roots
title_full_unstemmed Integrated Transcript and Metabolite Profiles Reveal That EbCHI Plays an Important Role in Scutellarin Accumulation in Erigeron breviscapus Hairy Roots
title_short Integrated Transcript and Metabolite Profiles Reveal That EbCHI Plays an Important Role in Scutellarin Accumulation in Erigeron breviscapus Hairy Roots
title_sort integrated transcript and metabolite profiles reveal that ebchi plays an important role in scutellarin accumulation in erigeron breviscapus hairy roots
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6036287/
https://www.ncbi.nlm.nih.gov/pubmed/30013578
http://dx.doi.org/10.3389/fpls.2018.00789
work_keys_str_mv AT chenruibing integratedtranscriptandmetaboliteprofilesrevealthatebchiplaysanimportantroleinscutellarinaccumulationinerigeronbreviscapushairyroots
AT chenxianghui integratedtranscriptandmetaboliteprofilesrevealthatebchiplaysanimportantroleinscutellarinaccumulationinerigeronbreviscapushairyroots
AT zhutingting integratedtranscriptandmetaboliteprofilesrevealthatebchiplaysanimportantroleinscutellarinaccumulationinerigeronbreviscapushairyroots
AT liujianghua integratedtranscriptandmetaboliteprofilesrevealthatebchiplaysanimportantroleinscutellarinaccumulationinerigeronbreviscapushairyroots
AT xiangxing integratedtranscriptandmetaboliteprofilesrevealthatebchiplaysanimportantroleinscutellarinaccumulationinerigeronbreviscapushairyroots
AT yujian integratedtranscriptandmetaboliteprofilesrevealthatebchiplaysanimportantroleinscutellarinaccumulationinerigeronbreviscapushairyroots
AT tanhexin integratedtranscriptandmetaboliteprofilesrevealthatebchiplaysanimportantroleinscutellarinaccumulationinerigeronbreviscapushairyroots
AT gaoshouhong integratedtranscriptandmetaboliteprofilesrevealthatebchiplaysanimportantroleinscutellarinaccumulationinerigeronbreviscapushairyroots
AT liqing integratedtranscriptandmetaboliteprofilesrevealthatebchiplaysanimportantroleinscutellarinaccumulationinerigeronbreviscapushairyroots
AT fangyichao integratedtranscriptandmetaboliteprofilesrevealthatebchiplaysanimportantroleinscutellarinaccumulationinerigeronbreviscapushairyroots
AT chenwansheng integratedtranscriptandmetaboliteprofilesrevealthatebchiplaysanimportantroleinscutellarinaccumulationinerigeronbreviscapushairyroots
AT zhanglei integratedtranscriptandmetaboliteprofilesrevealthatebchiplaysanimportantroleinscutellarinaccumulationinerigeronbreviscapushairyroots
AT huangbaokang integratedtranscriptandmetaboliteprofilesrevealthatebchiplaysanimportantroleinscutellarinaccumulationinerigeronbreviscapushairyroots