Cargando…
Integrated Transcript and Metabolite Profiles Reveal That EbCHI Plays an Important Role in Scutellarin Accumulation in Erigeron breviscapus Hairy Roots
Scutellarin, a flavonoid 7-O-glucuronide, is an essential bioactive compound of Erigeron breviscapus (Vaniot) Hand.-Mazz. used for the treatment of cerebrovascular diseases. However, due to overexploitation and overuse, E. breviscapus is facing the problems of extinction and habitat degradation. In...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6036287/ https://www.ncbi.nlm.nih.gov/pubmed/30013578 http://dx.doi.org/10.3389/fpls.2018.00789 |
_version_ | 1783338137200623616 |
---|---|
author | Chen, Ruibing Chen, Xianghui Zhu, Tingting Liu, Jianghua Xiang, Xing Yu, Jian Tan, Hexin Gao, Shouhong Li, Qing Fang, Yichao Chen, Wansheng Zhang, Lei Huang, Baokang |
author_facet | Chen, Ruibing Chen, Xianghui Zhu, Tingting Liu, Jianghua Xiang, Xing Yu, Jian Tan, Hexin Gao, Shouhong Li, Qing Fang, Yichao Chen, Wansheng Zhang, Lei Huang, Baokang |
author_sort | Chen, Ruibing |
collection | PubMed |
description | Scutellarin, a flavonoid 7-O-glucuronide, is an essential bioactive compound of Erigeron breviscapus (Vaniot) Hand.-Mazz. used for the treatment of cerebrovascular diseases. However, due to overexploitation and overuse, E. breviscapus is facing the problems of extinction and habitat degradation. In this study, a correlation analysis between the transcript and metabolite profiles of methyl jasmonate (MeJA)-treated E. breviscapus at different time points indicated that chalcone isomerase (EbCHI) was the primary contributor to scutellarin accumulation during flavonoid biosynthesis. EbCHI was then further characterized as a chalcone isomerase that efficiently converted chalcone to naringenin in vitro. Optimal parameters derived by comparing different culture conditions were successfully used to establish hairy root cultures of E. breviscapus with a maximum transformation rate of 60% in B5 medium. Furthermore, overexpression of EbCHI significantly enhanced scutellarin accumulation in E. breviscapus hairy roots with a maximum content of 2.21 mg g(-1) (dw), 10-fold higher than that of natural roots (0.21 mg g(-1) dw). This study sheds new light on a method of effective gene-based metabolic engineering by accurate and appropriate strategies and provides a protocol for hairy root cultures that accumulate high levels of scutellarin, providing a promising prospect for relieving the overexploitation and unavailability of E. breviscapus in the future. |
format | Online Article Text |
id | pubmed-6036287 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-60362872018-07-16 Integrated Transcript and Metabolite Profiles Reveal That EbCHI Plays an Important Role in Scutellarin Accumulation in Erigeron breviscapus Hairy Roots Chen, Ruibing Chen, Xianghui Zhu, Tingting Liu, Jianghua Xiang, Xing Yu, Jian Tan, Hexin Gao, Shouhong Li, Qing Fang, Yichao Chen, Wansheng Zhang, Lei Huang, Baokang Front Plant Sci Plant Science Scutellarin, a flavonoid 7-O-glucuronide, is an essential bioactive compound of Erigeron breviscapus (Vaniot) Hand.-Mazz. used for the treatment of cerebrovascular diseases. However, due to overexploitation and overuse, E. breviscapus is facing the problems of extinction and habitat degradation. In this study, a correlation analysis between the transcript and metabolite profiles of methyl jasmonate (MeJA)-treated E. breviscapus at different time points indicated that chalcone isomerase (EbCHI) was the primary contributor to scutellarin accumulation during flavonoid biosynthesis. EbCHI was then further characterized as a chalcone isomerase that efficiently converted chalcone to naringenin in vitro. Optimal parameters derived by comparing different culture conditions were successfully used to establish hairy root cultures of E. breviscapus with a maximum transformation rate of 60% in B5 medium. Furthermore, overexpression of EbCHI significantly enhanced scutellarin accumulation in E. breviscapus hairy roots with a maximum content of 2.21 mg g(-1) (dw), 10-fold higher than that of natural roots (0.21 mg g(-1) dw). This study sheds new light on a method of effective gene-based metabolic engineering by accurate and appropriate strategies and provides a protocol for hairy root cultures that accumulate high levels of scutellarin, providing a promising prospect for relieving the overexploitation and unavailability of E. breviscapus in the future. Frontiers Media S.A. 2018-06-21 /pmc/articles/PMC6036287/ /pubmed/30013578 http://dx.doi.org/10.3389/fpls.2018.00789 Text en Copyright © 2018 Chen, Chen, Zhu, Liu, Xiang, Yu, Tan, Gao, Li, Fang, Chen, Zhang and Huang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Chen, Ruibing Chen, Xianghui Zhu, Tingting Liu, Jianghua Xiang, Xing Yu, Jian Tan, Hexin Gao, Shouhong Li, Qing Fang, Yichao Chen, Wansheng Zhang, Lei Huang, Baokang Integrated Transcript and Metabolite Profiles Reveal That EbCHI Plays an Important Role in Scutellarin Accumulation in Erigeron breviscapus Hairy Roots |
title | Integrated Transcript and Metabolite Profiles Reveal That EbCHI Plays an Important Role in Scutellarin Accumulation in Erigeron breviscapus Hairy Roots |
title_full | Integrated Transcript and Metabolite Profiles Reveal That EbCHI Plays an Important Role in Scutellarin Accumulation in Erigeron breviscapus Hairy Roots |
title_fullStr | Integrated Transcript and Metabolite Profiles Reveal That EbCHI Plays an Important Role in Scutellarin Accumulation in Erigeron breviscapus Hairy Roots |
title_full_unstemmed | Integrated Transcript and Metabolite Profiles Reveal That EbCHI Plays an Important Role in Scutellarin Accumulation in Erigeron breviscapus Hairy Roots |
title_short | Integrated Transcript and Metabolite Profiles Reveal That EbCHI Plays an Important Role in Scutellarin Accumulation in Erigeron breviscapus Hairy Roots |
title_sort | integrated transcript and metabolite profiles reveal that ebchi plays an important role in scutellarin accumulation in erigeron breviscapus hairy roots |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6036287/ https://www.ncbi.nlm.nih.gov/pubmed/30013578 http://dx.doi.org/10.3389/fpls.2018.00789 |
work_keys_str_mv | AT chenruibing integratedtranscriptandmetaboliteprofilesrevealthatebchiplaysanimportantroleinscutellarinaccumulationinerigeronbreviscapushairyroots AT chenxianghui integratedtranscriptandmetaboliteprofilesrevealthatebchiplaysanimportantroleinscutellarinaccumulationinerigeronbreviscapushairyroots AT zhutingting integratedtranscriptandmetaboliteprofilesrevealthatebchiplaysanimportantroleinscutellarinaccumulationinerigeronbreviscapushairyroots AT liujianghua integratedtranscriptandmetaboliteprofilesrevealthatebchiplaysanimportantroleinscutellarinaccumulationinerigeronbreviscapushairyroots AT xiangxing integratedtranscriptandmetaboliteprofilesrevealthatebchiplaysanimportantroleinscutellarinaccumulationinerigeronbreviscapushairyroots AT yujian integratedtranscriptandmetaboliteprofilesrevealthatebchiplaysanimportantroleinscutellarinaccumulationinerigeronbreviscapushairyroots AT tanhexin integratedtranscriptandmetaboliteprofilesrevealthatebchiplaysanimportantroleinscutellarinaccumulationinerigeronbreviscapushairyroots AT gaoshouhong integratedtranscriptandmetaboliteprofilesrevealthatebchiplaysanimportantroleinscutellarinaccumulationinerigeronbreviscapushairyroots AT liqing integratedtranscriptandmetaboliteprofilesrevealthatebchiplaysanimportantroleinscutellarinaccumulationinerigeronbreviscapushairyroots AT fangyichao integratedtranscriptandmetaboliteprofilesrevealthatebchiplaysanimportantroleinscutellarinaccumulationinerigeronbreviscapushairyroots AT chenwansheng integratedtranscriptandmetaboliteprofilesrevealthatebchiplaysanimportantroleinscutellarinaccumulationinerigeronbreviscapushairyroots AT zhanglei integratedtranscriptandmetaboliteprofilesrevealthatebchiplaysanimportantroleinscutellarinaccumulationinerigeronbreviscapushairyroots AT huangbaokang integratedtranscriptandmetaboliteprofilesrevealthatebchiplaysanimportantroleinscutellarinaccumulationinerigeronbreviscapushairyroots |