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Culture conditions influence satellite cell activation and survival of single myofibers

Single myofiber isolation protocols allow to obtain an in vitro system in which the physical association between the myofiber and its stem cells, the satellite cells, is adequately preserved. This technique is an indispensable tool by which the muscle regeneration process can be recapitulated and st...

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Autores principales: Renzini, Alessandra, Benedetti, Anna, Bouchè, Marina, Silvestroni, Leopoldo, Adamo, Sergio, Moresi, Viviana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications, Pavia, Italy 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6036316/
https://www.ncbi.nlm.nih.gov/pubmed/29991990
http://dx.doi.org/10.4081/ejtm.2018.7567
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author Renzini, Alessandra
Benedetti, Anna
Bouchè, Marina
Silvestroni, Leopoldo
Adamo, Sergio
Moresi, Viviana
author_facet Renzini, Alessandra
Benedetti, Anna
Bouchè, Marina
Silvestroni, Leopoldo
Adamo, Sergio
Moresi, Viviana
author_sort Renzini, Alessandra
collection PubMed
description Single myofiber isolation protocols allow to obtain an in vitro system in which the physical association between the myofiber and its stem cells, the satellite cells, is adequately preserved. This technique is an indispensable tool by which the muscle regeneration process can be recapitulated and studied in each specific phase, from satellite cell activation to proliferation, from differentiation to fusion. This study aims to clarify the effect of different culture conditions on single myofibers, their associated satellite cells, and the physiological behavior of the satellite cells upon long term culture. By direct observations of the cultures, we compared different experimental conditions and their effect on both satellite cell behavior and myofiber viability.
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spelling pubmed-60363162018-07-10 Culture conditions influence satellite cell activation and survival of single myofibers Renzini, Alessandra Benedetti, Anna Bouchè, Marina Silvestroni, Leopoldo Adamo, Sergio Moresi, Viviana Eur J Transl Myol Article Single myofiber isolation protocols allow to obtain an in vitro system in which the physical association between the myofiber and its stem cells, the satellite cells, is adequately preserved. This technique is an indispensable tool by which the muscle regeneration process can be recapitulated and studied in each specific phase, from satellite cell activation to proliferation, from differentiation to fusion. This study aims to clarify the effect of different culture conditions on single myofibers, their associated satellite cells, and the physiological behavior of the satellite cells upon long term culture. By direct observations of the cultures, we compared different experimental conditions and their effect on both satellite cell behavior and myofiber viability. PAGEPress Publications, Pavia, Italy 2018-05-29 /pmc/articles/PMC6036316/ /pubmed/29991990 http://dx.doi.org/10.4081/ejtm.2018.7567 Text en http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License (by-nc 4.0) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Article
Renzini, Alessandra
Benedetti, Anna
Bouchè, Marina
Silvestroni, Leopoldo
Adamo, Sergio
Moresi, Viviana
Culture conditions influence satellite cell activation and survival of single myofibers
title Culture conditions influence satellite cell activation and survival of single myofibers
title_full Culture conditions influence satellite cell activation and survival of single myofibers
title_fullStr Culture conditions influence satellite cell activation and survival of single myofibers
title_full_unstemmed Culture conditions influence satellite cell activation and survival of single myofibers
title_short Culture conditions influence satellite cell activation and survival of single myofibers
title_sort culture conditions influence satellite cell activation and survival of single myofibers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6036316/
https://www.ncbi.nlm.nih.gov/pubmed/29991990
http://dx.doi.org/10.4081/ejtm.2018.7567
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