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miR-205-5p inhibits cell migration and invasion in prostatic carcinoma by targeting ZEB1
Many studies have demonstrated that miRNAs have influence on tumorigenesis and progression of human cancers, including invasion and migration. Thus, the role of miR-205/ZEB1 axis for the migration and invasion of prostate cancer cells was explored in the present study. The miR-205-5p and zinc finger...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6036508/ https://www.ncbi.nlm.nih.gov/pubmed/30008858 http://dx.doi.org/10.3892/ol.2018.8862 |
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author | Li, Lianpeng Li, Shouqiang |
author_facet | Li, Lianpeng Li, Shouqiang |
author_sort | Li, Lianpeng |
collection | PubMed |
description | Many studies have demonstrated that miRNAs have influence on tumorigenesis and progression of human cancers, including invasion and migration. Thus, the role of miR-205/ZEB1 axis for the migration and invasion of prostate cancer cells was explored in the present study. The miR-205-5p and zinc finger E-box binding homeobox 1 (ZEB1) mRNA expression levels were observed in prostate cancer tissues or cell lines via reverse transcription-quantitative PCR (RT-qPCR), and the protein level of ZEB1 was measured by western blotting. Dual-Luciferase Reporter Assay was used to verify the relationship between miR-205-5p and ZEB1. In addition, cell migration and invasion was measured by Transwell assay. The results revealed that, compared with the control, downregulation of miR-205-5p was detected in prostate cancer tissues and cell lines, and miR-205-5p overexpression was found to inhibit cell migration and invasion. Moreover, miR-205-5p was confirmed to directly target ZEB1 in prostate cancer. Importantly, ZEB1 was identified to weaken the inhibitory effect of miR-205-5p in prostate cancer. In conclusion, miR-205-5p inhibited cell migration and invasion in prostatic carcinoma by targeting ZEB1 and miR-205-5p/ZEB1 axis shows potential to be developed in therapeutic strategies for prostate cancer. |
format | Online Article Text |
id | pubmed-6036508 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-60365082018-07-15 miR-205-5p inhibits cell migration and invasion in prostatic carcinoma by targeting ZEB1 Li, Lianpeng Li, Shouqiang Oncol Lett Articles Many studies have demonstrated that miRNAs have influence on tumorigenesis and progression of human cancers, including invasion and migration. Thus, the role of miR-205/ZEB1 axis for the migration and invasion of prostate cancer cells was explored in the present study. The miR-205-5p and zinc finger E-box binding homeobox 1 (ZEB1) mRNA expression levels were observed in prostate cancer tissues or cell lines via reverse transcription-quantitative PCR (RT-qPCR), and the protein level of ZEB1 was measured by western blotting. Dual-Luciferase Reporter Assay was used to verify the relationship between miR-205-5p and ZEB1. In addition, cell migration and invasion was measured by Transwell assay. The results revealed that, compared with the control, downregulation of miR-205-5p was detected in prostate cancer tissues and cell lines, and miR-205-5p overexpression was found to inhibit cell migration and invasion. Moreover, miR-205-5p was confirmed to directly target ZEB1 in prostate cancer. Importantly, ZEB1 was identified to weaken the inhibitory effect of miR-205-5p in prostate cancer. In conclusion, miR-205-5p inhibited cell migration and invasion in prostatic carcinoma by targeting ZEB1 and miR-205-5p/ZEB1 axis shows potential to be developed in therapeutic strategies for prostate cancer. D.A. Spandidos 2018-08 2018-05-31 /pmc/articles/PMC6036508/ /pubmed/30008858 http://dx.doi.org/10.3892/ol.2018.8862 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Li, Lianpeng Li, Shouqiang miR-205-5p inhibits cell migration and invasion in prostatic carcinoma by targeting ZEB1 |
title | miR-205-5p inhibits cell migration and invasion in prostatic carcinoma by targeting ZEB1 |
title_full | miR-205-5p inhibits cell migration and invasion in prostatic carcinoma by targeting ZEB1 |
title_fullStr | miR-205-5p inhibits cell migration and invasion in prostatic carcinoma by targeting ZEB1 |
title_full_unstemmed | miR-205-5p inhibits cell migration and invasion in prostatic carcinoma by targeting ZEB1 |
title_short | miR-205-5p inhibits cell migration and invasion in prostatic carcinoma by targeting ZEB1 |
title_sort | mir-205-5p inhibits cell migration and invasion in prostatic carcinoma by targeting zeb1 |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6036508/ https://www.ncbi.nlm.nih.gov/pubmed/30008858 http://dx.doi.org/10.3892/ol.2018.8862 |
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