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A “Hole Punched Plate” method for easy generation and harvesting of microconidia in the dermatophyte Trichophyton rubrum

Handling of the medically important dermatophyte Trichophyton rubrum in the laboratory typically requires the generation of spores — for storage, treatment and plating when needed. The described method allows technically simple but efficient generation and harvesting of microconidia by cutting holes...

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Detalles Bibliográficos
Autor principal: Siede, Wolfram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6036861/
https://www.ncbi.nlm.nih.gov/pubmed/29992193
http://dx.doi.org/10.1016/j.heliyon.2018.e00676
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author Siede, Wolfram
author_facet Siede, Wolfram
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description Handling of the medically important dermatophyte Trichophyton rubrum in the laboratory typically requires the generation of spores — for storage, treatment and plating when needed. The described method allows technically simple but efficient generation and harvesting of microconidia by cutting holes in Sabouraud dextrose agar medium that is covered by a mature T. rubrum mycelium.
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spelling pubmed-60368612018-07-10 A “Hole Punched Plate” method for easy generation and harvesting of microconidia in the dermatophyte Trichophyton rubrum Siede, Wolfram Heliyon Article Handling of the medically important dermatophyte Trichophyton rubrum in the laboratory typically requires the generation of spores — for storage, treatment and plating when needed. The described method allows technically simple but efficient generation and harvesting of microconidia by cutting holes in Sabouraud dextrose agar medium that is covered by a mature T. rubrum mycelium. Elsevier 2018-07-05 /pmc/articles/PMC6036861/ /pubmed/29992193 http://dx.doi.org/10.1016/j.heliyon.2018.e00676 Text en © 2018 The Author http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Siede, Wolfram
A “Hole Punched Plate” method for easy generation and harvesting of microconidia in the dermatophyte Trichophyton rubrum
title A “Hole Punched Plate” method for easy generation and harvesting of microconidia in the dermatophyte Trichophyton rubrum
title_full A “Hole Punched Plate” method for easy generation and harvesting of microconidia in the dermatophyte Trichophyton rubrum
title_fullStr A “Hole Punched Plate” method for easy generation and harvesting of microconidia in the dermatophyte Trichophyton rubrum
title_full_unstemmed A “Hole Punched Plate” method for easy generation and harvesting of microconidia in the dermatophyte Trichophyton rubrum
title_short A “Hole Punched Plate” method for easy generation and harvesting of microconidia in the dermatophyte Trichophyton rubrum
title_sort “hole punched plate” method for easy generation and harvesting of microconidia in the dermatophyte trichophyton rubrum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6036861/
https://www.ncbi.nlm.nih.gov/pubmed/29992193
http://dx.doi.org/10.1016/j.heliyon.2018.e00676
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