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Evaluation and validation of an alternative method to detect Campylobacter spp. in dairy products

Foods implicated in human campylobacteriosis include raw or undercooked poultry and raw dairy products. Because Campylobacter spp. are the most frequently reported cause of bacterial infection in the European Union and because conventional methods are cumbersome, rapid methods for Campylobacter dete...

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Autores principales: Razzuoli, Elisabetta, Vencia, Walter, Fedele, Valeria, Mignone, Giulia, Lazzara, Fabrizio, Rubini, Danja, Vito, Guendalina, Porcario, Chiara, Bozzetta, Elena, Ferrari, Angelo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications, Pavia, Italy 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6036990/
https://www.ncbi.nlm.nih.gov/pubmed/30046558
http://dx.doi.org/10.4081/ijfs.2018.7180
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author Razzuoli, Elisabetta
Vencia, Walter
Fedele, Valeria
Mignone, Giulia
Lazzara, Fabrizio
Rubini, Danja
Vito, Guendalina
Porcario, Chiara
Bozzetta, Elena
Ferrari, Angelo
author_facet Razzuoli, Elisabetta
Vencia, Walter
Fedele, Valeria
Mignone, Giulia
Lazzara, Fabrizio
Rubini, Danja
Vito, Guendalina
Porcario, Chiara
Bozzetta, Elena
Ferrari, Angelo
author_sort Razzuoli, Elisabetta
collection PubMed
description Foods implicated in human campylobacteriosis include raw or undercooked poultry and raw dairy products. Because Campylobacter spp. are the most frequently reported cause of bacterial infection in the European Union and because conventional methods are cumbersome, rapid methods for Campylobacter detection and quantification in food are needed. With this study we sought to validate, according to the standard procedure (UNI EN ISO 16140:2003), an alternative to the reference analytical method (UNI EN ISO 10272-1:2006) for official controls of Campylobacter spp. in raw milk and dairy products. Milk samples collected from 16 milk vending machines located throughout the Genoa metropolitan area were analyzed using two different methods, an enzyme-linked fluorescent assay (ELFA) and a real-time PCR assay, and evaluated in parallel against the reference method. In addition, a total of 460 samples of raw milk collected from milk vending machines were analyzed by ELFA. Results obtained with ELFA showed it was compliant with UNI EN ISO 10272-1:2006 criteria and that the immunoassay had 100% sensitivity, specificity, and accuracy. Regarding samples of milk vending machines, 5.0% (23/460) tested positive at ELFA screening and were subsequently confirmed as C. jejuni. Validation according to UNI EN ISO 16140:2003 of the ELFA method suggests it may be a useful alternative to conventional methods for detecting Campylobacter spp. in official controls.
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spelling pubmed-60369902018-07-25 Evaluation and validation of an alternative method to detect Campylobacter spp. in dairy products Razzuoli, Elisabetta Vencia, Walter Fedele, Valeria Mignone, Giulia Lazzara, Fabrizio Rubini, Danja Vito, Guendalina Porcario, Chiara Bozzetta, Elena Ferrari, Angelo Ital J Food Saf Article Foods implicated in human campylobacteriosis include raw or undercooked poultry and raw dairy products. Because Campylobacter spp. are the most frequently reported cause of bacterial infection in the European Union and because conventional methods are cumbersome, rapid methods for Campylobacter detection and quantification in food are needed. With this study we sought to validate, according to the standard procedure (UNI EN ISO 16140:2003), an alternative to the reference analytical method (UNI EN ISO 10272-1:2006) for official controls of Campylobacter spp. in raw milk and dairy products. Milk samples collected from 16 milk vending machines located throughout the Genoa metropolitan area were analyzed using two different methods, an enzyme-linked fluorescent assay (ELFA) and a real-time PCR assay, and evaluated in parallel against the reference method. In addition, a total of 460 samples of raw milk collected from milk vending machines were analyzed by ELFA. Results obtained with ELFA showed it was compliant with UNI EN ISO 10272-1:2006 criteria and that the immunoassay had 100% sensitivity, specificity, and accuracy. Regarding samples of milk vending machines, 5.0% (23/460) tested positive at ELFA screening and were subsequently confirmed as C. jejuni. Validation according to UNI EN ISO 16140:2003 of the ELFA method suggests it may be a useful alternative to conventional methods for detecting Campylobacter spp. in official controls. PAGEPress Publications, Pavia, Italy 2018-07-03 /pmc/articles/PMC6036990/ /pubmed/30046558 http://dx.doi.org/10.4081/ijfs.2018.7180 Text en ©Copyright E. Razzuoli et al., 2018 http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Razzuoli, Elisabetta
Vencia, Walter
Fedele, Valeria
Mignone, Giulia
Lazzara, Fabrizio
Rubini, Danja
Vito, Guendalina
Porcario, Chiara
Bozzetta, Elena
Ferrari, Angelo
Evaluation and validation of an alternative method to detect Campylobacter spp. in dairy products
title Evaluation and validation of an alternative method to detect Campylobacter spp. in dairy products
title_full Evaluation and validation of an alternative method to detect Campylobacter spp. in dairy products
title_fullStr Evaluation and validation of an alternative method to detect Campylobacter spp. in dairy products
title_full_unstemmed Evaluation and validation of an alternative method to detect Campylobacter spp. in dairy products
title_short Evaluation and validation of an alternative method to detect Campylobacter spp. in dairy products
title_sort evaluation and validation of an alternative method to detect campylobacter spp. in dairy products
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6036990/
https://www.ncbi.nlm.nih.gov/pubmed/30046558
http://dx.doi.org/10.4081/ijfs.2018.7180
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