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DNA barcoding for the verification of supplier’s compliance in the seafood chain: How the lab can support companies in ensuring traceability
Food Business Operators (FBOs) rely on laboratory analysis to ensure seafood traceability. DNA barcoding and Forensically Informative Nucleotide Sequencing may represent a support within self-checking programs finalized to suppliers’ qualification and products identity certification. The present stu...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PAGEPress Publications, Pavia, Italy
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6037000/ https://www.ncbi.nlm.nih.gov/pubmed/30046552 http://dx.doi.org/10.4081/ijfs.2018.6894 |
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author | Tinacci, Lara Guidi, Alessandra Toto, Andrea Guardone, Lisa Giusti, Alice D’Amico, Priscilla Armani, Andrea |
author_facet | Tinacci, Lara Guidi, Alessandra Toto, Andrea Guardone, Lisa Giusti, Alice D’Amico, Priscilla Armani, Andrea |
author_sort | Tinacci, Lara |
collection | PubMed |
description | Food Business Operators (FBOs) rely on laboratory analysis to ensure seafood traceability. DNA barcoding and Forensically Informative Nucleotide Sequencing may represent a support within self-checking programs finalized to suppliers’ qualification and products identity certification. The present study aimed at verifying the usefulness of a decisional procedure (decision tree) set up at the FishLab (Department of Veterinary Sciences, University of Pisa, Italy) for seafood species identification by DNA analysis, to cope with FBOs’ needs. The decision tree was applied to the analysis of 182 seafood (fish and molluscs) products, conferred to the FishLab by different FBOs between 2014 and 2015 as result of their self-checking activities. The analysis relied on a standard COI gene fragment eventually integrated by the analysis of alternative or supportive molecular targets (cytb and 16S rRNA). It also included a mini-DNA barcoding approach for processed products. Overall, 96.2% of the samples were unambiguously identified at species level using the elective target alone (92.4%) or a multi-target approach (3.8%). The lack of species identification (3.8%) was attributable to the absence of reference sequences or to the low resolution of the molecular targets. Nonetheless, all the molecular results were deemed adequate to evaluate the sample’s compliance to the label information. Non-compliances were highlighted in 18.1% of the products. The protocol was proven as an effective supportive tool for the seafood identity verification within the supply chain self-checking activities. In addition, a considerable fraud rate was confirmed and the species most frequently involved in substitution were pointed out. |
format | Online Article Text |
id | pubmed-6037000 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | PAGEPress Publications, Pavia, Italy |
record_format | MEDLINE/PubMed |
spelling | pubmed-60370002018-07-25 DNA barcoding for the verification of supplier’s compliance in the seafood chain: How the lab can support companies in ensuring traceability Tinacci, Lara Guidi, Alessandra Toto, Andrea Guardone, Lisa Giusti, Alice D’Amico, Priscilla Armani, Andrea Ital J Food Saf Article Food Business Operators (FBOs) rely on laboratory analysis to ensure seafood traceability. DNA barcoding and Forensically Informative Nucleotide Sequencing may represent a support within self-checking programs finalized to suppliers’ qualification and products identity certification. The present study aimed at verifying the usefulness of a decisional procedure (decision tree) set up at the FishLab (Department of Veterinary Sciences, University of Pisa, Italy) for seafood species identification by DNA analysis, to cope with FBOs’ needs. The decision tree was applied to the analysis of 182 seafood (fish and molluscs) products, conferred to the FishLab by different FBOs between 2014 and 2015 as result of their self-checking activities. The analysis relied on a standard COI gene fragment eventually integrated by the analysis of alternative or supportive molecular targets (cytb and 16S rRNA). It also included a mini-DNA barcoding approach for processed products. Overall, 96.2% of the samples were unambiguously identified at species level using the elective target alone (92.4%) or a multi-target approach (3.8%). The lack of species identification (3.8%) was attributable to the absence of reference sequences or to the low resolution of the molecular targets. Nonetheless, all the molecular results were deemed adequate to evaluate the sample’s compliance to the label information. Non-compliances were highlighted in 18.1% of the products. The protocol was proven as an effective supportive tool for the seafood identity verification within the supply chain self-checking activities. In addition, a considerable fraud rate was confirmed and the species most frequently involved in substitution were pointed out. PAGEPress Publications, Pavia, Italy 2018-07-03 /pmc/articles/PMC6037000/ /pubmed/30046552 http://dx.doi.org/10.4081/ijfs.2018.6894 Text en ©Copyright L. Tinacci et al., 2018 http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Tinacci, Lara Guidi, Alessandra Toto, Andrea Guardone, Lisa Giusti, Alice D’Amico, Priscilla Armani, Andrea DNA barcoding for the verification of supplier’s compliance in the seafood chain: How the lab can support companies in ensuring traceability |
title | DNA barcoding for the verification of supplier’s compliance in the seafood chain: How the lab can support companies in ensuring traceability |
title_full | DNA barcoding for the verification of supplier’s compliance in the seafood chain: How the lab can support companies in ensuring traceability |
title_fullStr | DNA barcoding for the verification of supplier’s compliance in the seafood chain: How the lab can support companies in ensuring traceability |
title_full_unstemmed | DNA barcoding for the verification of supplier’s compliance in the seafood chain: How the lab can support companies in ensuring traceability |
title_short | DNA barcoding for the verification of supplier’s compliance in the seafood chain: How the lab can support companies in ensuring traceability |
title_sort | dna barcoding for the verification of supplier’s compliance in the seafood chain: how the lab can support companies in ensuring traceability |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6037000/ https://www.ncbi.nlm.nih.gov/pubmed/30046552 http://dx.doi.org/10.4081/ijfs.2018.6894 |
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