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Comparative Analysis of Ileal and Cecal Microbiota in Aged Rats

BACKGROUND: Gut microbiota contributes to intestinal and immune homeostasis through host-microbiota interactions. Distribution of the gut microbiota differs according to the location in the gastrointestinal tract. Although the microbiota properties change with age, evidence for the regional differen...

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Autores principales: Lee, Sun Min, Kim, Nayoung, Park, Ji Hyun, Nam, Ryoung Hee, Yoon, Kichul, Lee, Dong Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Cancer Prevention 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6037205/
https://www.ncbi.nlm.nih.gov/pubmed/30003066
http://dx.doi.org/10.15430/JCP.2018.23.2.70
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author Lee, Sun Min
Kim, Nayoung
Park, Ji Hyun
Nam, Ryoung Hee
Yoon, Kichul
Lee, Dong Ho
author_facet Lee, Sun Min
Kim, Nayoung
Park, Ji Hyun
Nam, Ryoung Hee
Yoon, Kichul
Lee, Dong Ho
author_sort Lee, Sun Min
collection PubMed
description BACKGROUND: Gut microbiota contributes to intestinal and immune homeostasis through host-microbiota interactions. Distribution of the gut microbiota differs according to the location in the gastrointestinal tract. Although the microbiota properties change with age, evidence for the regional difference of gut microbiota has been restricted to the young. The aim of this study is to compare the gut microbiota between terminal ileum and cecum of old rats. METHODS: We analyzed gut microbiome of luminal contents from ileum and cecum of 74-week-old and 2-year-old rats (corresponding to 60-year and 80-year-old of human age) by metagenome sequencing of 16S rRNA. RESULTS: Inter-individual variation (beta diversity) of microbiota was higher in ileum than in cecum. Conversely, alpha diversity of microbiota composition was higher in cecum than in ileum. Lactobacillaceae were more abundant in ileum compared to cecum while Ruminococcaceae and Lachnospiraceae were more enriched in cecum. The proportions of Deltaproteobacteria were increased in cecal microbiota of 2-year-old rats compared to 74-week-old rats. CONCLUSIONS: Major regional distinctions of microbiota between ileum and cecum of old rats appear consistent with those of young rats. Age-related alterations of gut microbiota in old rats seem to occur in minor compositions.
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spelling pubmed-60372052018-07-12 Comparative Analysis of Ileal and Cecal Microbiota in Aged Rats Lee, Sun Min Kim, Nayoung Park, Ji Hyun Nam, Ryoung Hee Yoon, Kichul Lee, Dong Ho J Cancer Prev Original Article BACKGROUND: Gut microbiota contributes to intestinal and immune homeostasis through host-microbiota interactions. Distribution of the gut microbiota differs according to the location in the gastrointestinal tract. Although the microbiota properties change with age, evidence for the regional difference of gut microbiota has been restricted to the young. The aim of this study is to compare the gut microbiota between terminal ileum and cecum of old rats. METHODS: We analyzed gut microbiome of luminal contents from ileum and cecum of 74-week-old and 2-year-old rats (corresponding to 60-year and 80-year-old of human age) by metagenome sequencing of 16S rRNA. RESULTS: Inter-individual variation (beta diversity) of microbiota was higher in ileum than in cecum. Conversely, alpha diversity of microbiota composition was higher in cecum than in ileum. Lactobacillaceae were more abundant in ileum compared to cecum while Ruminococcaceae and Lachnospiraceae were more enriched in cecum. The proportions of Deltaproteobacteria were increased in cecal microbiota of 2-year-old rats compared to 74-week-old rats. CONCLUSIONS: Major regional distinctions of microbiota between ileum and cecum of old rats appear consistent with those of young rats. Age-related alterations of gut microbiota in old rats seem to occur in minor compositions. Korean Society of Cancer Prevention 2018-06 2018-06-30 /pmc/articles/PMC6037205/ /pubmed/30003066 http://dx.doi.org/10.15430/JCP.2018.23.2.70 Text en Copyright © 2018 Korean Society of Cancer Prevention This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Lee, Sun Min
Kim, Nayoung
Park, Ji Hyun
Nam, Ryoung Hee
Yoon, Kichul
Lee, Dong Ho
Comparative Analysis of Ileal and Cecal Microbiota in Aged Rats
title Comparative Analysis of Ileal and Cecal Microbiota in Aged Rats
title_full Comparative Analysis of Ileal and Cecal Microbiota in Aged Rats
title_fullStr Comparative Analysis of Ileal and Cecal Microbiota in Aged Rats
title_full_unstemmed Comparative Analysis of Ileal and Cecal Microbiota in Aged Rats
title_short Comparative Analysis of Ileal and Cecal Microbiota in Aged Rats
title_sort comparative analysis of ileal and cecal microbiota in aged rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6037205/
https://www.ncbi.nlm.nih.gov/pubmed/30003066
http://dx.doi.org/10.15430/JCP.2018.23.2.70
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