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Vertex sliding drives intercalation by radial coupling of adhesion and actomyosin networks during Drosophila germband extension
Oriented cell intercalation is an essential developmental process that shapes tissue morphologies through the directional insertion of cells between their neighbors. Previous research has focused on properties of cell–cell interfaces, while the function of tricellular vertices has remained unaddress...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6037471/ https://www.ncbi.nlm.nih.gov/pubmed/29985789 http://dx.doi.org/10.7554/eLife.34586 |
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author | Vanderleest, Timothy E Smits, Celia M Xie, Yi Jewett, Cayla E Blankenship, J Todd Loerke, Dinah |
author_facet | Vanderleest, Timothy E Smits, Celia M Xie, Yi Jewett, Cayla E Blankenship, J Todd Loerke, Dinah |
author_sort | Vanderleest, Timothy E |
collection | PubMed |
description | Oriented cell intercalation is an essential developmental process that shapes tissue morphologies through the directional insertion of cells between their neighbors. Previous research has focused on properties of cell–cell interfaces, while the function of tricellular vertices has remained unaddressed. Here, we identify a highly novel mechanism in which vertices demonstrate independent sliding behaviors along cell peripheries to produce the topological deformations responsible for intercalation. Through systematic analysis, we find that the motion of vertices connected by contracting interfaces is not physically coupled, but instead possess strong radial coupling. E-cadherin and Myosin II exist in previously unstudied populations at cell vertices and undergo oscillatory cycles of accumulation and dispersion that are coordinated with changes in cell area. Additionally, peak enrichment of vertex E-cadherin/Myosin II coincides with interface length stabilization. Our results suggest a model in which asymmetric radial force balance directs the progressive, ratcheted motion of individual vertices to drive intercalation. |
format | Online Article Text |
id | pubmed-6037471 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-60374712018-07-11 Vertex sliding drives intercalation by radial coupling of adhesion and actomyosin networks during Drosophila germband extension Vanderleest, Timothy E Smits, Celia M Xie, Yi Jewett, Cayla E Blankenship, J Todd Loerke, Dinah eLife Computational and Systems Biology Oriented cell intercalation is an essential developmental process that shapes tissue morphologies through the directional insertion of cells between their neighbors. Previous research has focused on properties of cell–cell interfaces, while the function of tricellular vertices has remained unaddressed. Here, we identify a highly novel mechanism in which vertices demonstrate independent sliding behaviors along cell peripheries to produce the topological deformations responsible for intercalation. Through systematic analysis, we find that the motion of vertices connected by contracting interfaces is not physically coupled, but instead possess strong radial coupling. E-cadherin and Myosin II exist in previously unstudied populations at cell vertices and undergo oscillatory cycles of accumulation and dispersion that are coordinated with changes in cell area. Additionally, peak enrichment of vertex E-cadherin/Myosin II coincides with interface length stabilization. Our results suggest a model in which asymmetric radial force balance directs the progressive, ratcheted motion of individual vertices to drive intercalation. eLife Sciences Publications, Ltd 2018-07-09 /pmc/articles/PMC6037471/ /pubmed/29985789 http://dx.doi.org/10.7554/eLife.34586 Text en © 2018, Vanderleest et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Computational and Systems Biology Vanderleest, Timothy E Smits, Celia M Xie, Yi Jewett, Cayla E Blankenship, J Todd Loerke, Dinah Vertex sliding drives intercalation by radial coupling of adhesion and actomyosin networks during Drosophila germband extension |
title | Vertex sliding drives intercalation by radial coupling of adhesion and actomyosin networks during Drosophila germband extension |
title_full | Vertex sliding drives intercalation by radial coupling of adhesion and actomyosin networks during Drosophila germband extension |
title_fullStr | Vertex sliding drives intercalation by radial coupling of adhesion and actomyosin networks during Drosophila germband extension |
title_full_unstemmed | Vertex sliding drives intercalation by radial coupling of adhesion and actomyosin networks during Drosophila germband extension |
title_short | Vertex sliding drives intercalation by radial coupling of adhesion and actomyosin networks during Drosophila germband extension |
title_sort | vertex sliding drives intercalation by radial coupling of adhesion and actomyosin networks during drosophila germband extension |
topic | Computational and Systems Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6037471/ https://www.ncbi.nlm.nih.gov/pubmed/29985789 http://dx.doi.org/10.7554/eLife.34586 |
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