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Genetic editing and interrogation with Cpf1 and caged truncated pre-tRNA-like crRNA in mammalian cells

Cpf1, an RNA-guided DNA endonuclease that belongs to a new class II CRISPR system, has recently been harnessed for genome editing. Herein, we report an RNase-resistant caged truncated pre-tRNA-like crRNA (catRNA) that confers precise and efficient gene editing with the Lachnospiraceae bacterium Cpf1...

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Detalles Bibliográficos
Autores principales:	Zhang, Xuhua, Xu, Linping, Fan, Ruihua, Gao, Quanli, Song, Yunfeng, Lyu, Xiaodong, Ren, Jiangtao, Song, Yongping
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6037731/ https://www.ncbi.nlm.nih.gov/pubmed/30002872 http://dx.doi.org/10.1038/s41421-018-0035-0

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