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Comparative Phospho- and Acetyl Proteomics Analysis of Posttranslational Modifications Regulating Intestine Regeneration in Sea Cucumbers

Sea cucumbers exposed to stressful circumstances eviscerate most internal organs, and then regenerate them rapidly under favorable environments. Reversible protein phosphorylation and acetylation are major modifications regulating protein function. Herein, for the first time, we perform quantitative...

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Autores principales: Sun, Lina, Lin, Chenggang, Li, Xiaoni, Xing, Lili, Huo, Da, Sun, Jingchun, Zhang, Libin, Yang, Hongsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6037860/
https://www.ncbi.nlm.nih.gov/pubmed/30018572
http://dx.doi.org/10.3389/fphys.2018.00836
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author Sun, Lina
Lin, Chenggang
Li, Xiaoni
Xing, Lili
Huo, Da
Sun, Jingchun
Zhang, Libin
Yang, Hongsheng
author_facet Sun, Lina
Lin, Chenggang
Li, Xiaoni
Xing, Lili
Huo, Da
Sun, Jingchun
Zhang, Libin
Yang, Hongsheng
author_sort Sun, Lina
collection PubMed
description Sea cucumbers exposed to stressful circumstances eviscerate most internal organs, and then regenerate them rapidly under favorable environments. Reversible protein phosphorylation and acetylation are major modifications regulating protein function. Herein, for the first time, we perform quantitative phospho- and acetyl proteomics analyses of intestine regeneration in a sea cucumber species Apostichopus japonicus. We identified 1,862 phosphorylation sites in 1,169 proteins, and 712 acetylation sites in 470 proteins. Of the 147 and 251 proteins differentially modified by phosphorylation and acetylation, respectively, most were related to cytoskeleton biogenesis, protein synthesis and modification, signal recognition and transduction, energy production and conversion, or substance transport and metabolism. Phosphorylation appears to play a more important role in signal recognition and transduction than acetylation, while acetylation is of greater importance in posttranslational modification, protein turnover, chaperones; energy production and conversion; amino acid and lipid transport and metabolism. These results expanded our understanding of the regulatory mechanisms of posttranslational modifications in intestine regeneration of sea cucumbers after evisceration.
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spelling pubmed-60378602018-07-17 Comparative Phospho- and Acetyl Proteomics Analysis of Posttranslational Modifications Regulating Intestine Regeneration in Sea Cucumbers Sun, Lina Lin, Chenggang Li, Xiaoni Xing, Lili Huo, Da Sun, Jingchun Zhang, Libin Yang, Hongsheng Front Physiol Physiology Sea cucumbers exposed to stressful circumstances eviscerate most internal organs, and then regenerate them rapidly under favorable environments. Reversible protein phosphorylation and acetylation are major modifications regulating protein function. Herein, for the first time, we perform quantitative phospho- and acetyl proteomics analyses of intestine regeneration in a sea cucumber species Apostichopus japonicus. We identified 1,862 phosphorylation sites in 1,169 proteins, and 712 acetylation sites in 470 proteins. Of the 147 and 251 proteins differentially modified by phosphorylation and acetylation, respectively, most were related to cytoskeleton biogenesis, protein synthesis and modification, signal recognition and transduction, energy production and conversion, or substance transport and metabolism. Phosphorylation appears to play a more important role in signal recognition and transduction than acetylation, while acetylation is of greater importance in posttranslational modification, protein turnover, chaperones; energy production and conversion; amino acid and lipid transport and metabolism. These results expanded our understanding of the regulatory mechanisms of posttranslational modifications in intestine regeneration of sea cucumbers after evisceration. Frontiers Media S.A. 2018-07-03 /pmc/articles/PMC6037860/ /pubmed/30018572 http://dx.doi.org/10.3389/fphys.2018.00836 Text en Copyright © 2018 Sun, Lin, Li, Xing, Huo, Sun, Zhang and Yang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Sun, Lina
Lin, Chenggang
Li, Xiaoni
Xing, Lili
Huo, Da
Sun, Jingchun
Zhang, Libin
Yang, Hongsheng
Comparative Phospho- and Acetyl Proteomics Analysis of Posttranslational Modifications Regulating Intestine Regeneration in Sea Cucumbers
title Comparative Phospho- and Acetyl Proteomics Analysis of Posttranslational Modifications Regulating Intestine Regeneration in Sea Cucumbers
title_full Comparative Phospho- and Acetyl Proteomics Analysis of Posttranslational Modifications Regulating Intestine Regeneration in Sea Cucumbers
title_fullStr Comparative Phospho- and Acetyl Proteomics Analysis of Posttranslational Modifications Regulating Intestine Regeneration in Sea Cucumbers
title_full_unstemmed Comparative Phospho- and Acetyl Proteomics Analysis of Posttranslational Modifications Regulating Intestine Regeneration in Sea Cucumbers
title_short Comparative Phospho- and Acetyl Proteomics Analysis of Posttranslational Modifications Regulating Intestine Regeneration in Sea Cucumbers
title_sort comparative phospho- and acetyl proteomics analysis of posttranslational modifications regulating intestine regeneration in sea cucumbers
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6037860/
https://www.ncbi.nlm.nih.gov/pubmed/30018572
http://dx.doi.org/10.3389/fphys.2018.00836
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