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Study of seminal infection among an infertile male population in Qom, Iran, and its effect on sperm quality

BACKGROUND AND OBJECTIVES: There are very few analysis tools to examine seminal fluid specimens, so bacterial infections on male infertility has always been the subject of discussion. These infectious processes lead to deterioration of spermatogenesis, impairment of sperm function, and/or obstructio...

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Autores principales: Berjis, Katayon, Ghiasi, Mahdieh, Sangy, Sareh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6039451/
https://www.ncbi.nlm.nih.gov/pubmed/29997751
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author Berjis, Katayon
Ghiasi, Mahdieh
Sangy, Sareh
author_facet Berjis, Katayon
Ghiasi, Mahdieh
Sangy, Sareh
author_sort Berjis, Katayon
collection PubMed
description BACKGROUND AND OBJECTIVES: There are very few analysis tools to examine seminal fluid specimens, so bacterial infections on male infertility has always been the subject of discussion. These infectious processes lead to deterioration of spermatogenesis, impairment of sperm function, and/or obstruction of the seminal tract. In this study, we aimed at determining the role of bacterial infection on semen parameters including motility, count and normal morphology in infertile male patients. MATERIALS AND METHODS: In this cohort study, 150 infertile males having abnormal semen parameters (study group) and 150 healthy fertile males (control group) were included. A total of 300 semen samples were collected after 3 to 5 days of sexual abstinence. Volume, pH, concentration, normal morphology, and motility were evaluated. Samples were seeded using a calibrated loop on agar and EMB plates, which were incubated overnight. The microorganisms were identified by Gram staining technique, catalase and coagulase tests. RESULTS: The prevalence of seminal infection among infertile males in Qom was 21%. Among these infected samples 61.9%, 14.28%, 14.28% and 9.25% were contaminated with Staphylococcus aureus, coagulase negative staphylococci, Streptococcus and Escherichia coli, respectively. All the identified bacteria except Streptococcus caused a significant decrease in sperm concentration. Sperm motility was significantly lower in E. coli contaminated samples than in the control group, and the presence of E. coli and S. aureus led to a decline in normal morphology of the sperms. CONCLUSION: Sperm bacterial contamination is quite frequent and could contribute to the deterioration of the sperm quality of infertile males.
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spelling pubmed-60394512018-07-11 Study of seminal infection among an infertile male population in Qom, Iran, and its effect on sperm quality Berjis, Katayon Ghiasi, Mahdieh Sangy, Sareh Iran J Microbiol Original Article BACKGROUND AND OBJECTIVES: There are very few analysis tools to examine seminal fluid specimens, so bacterial infections on male infertility has always been the subject of discussion. These infectious processes lead to deterioration of spermatogenesis, impairment of sperm function, and/or obstruction of the seminal tract. In this study, we aimed at determining the role of bacterial infection on semen parameters including motility, count and normal morphology in infertile male patients. MATERIALS AND METHODS: In this cohort study, 150 infertile males having abnormal semen parameters (study group) and 150 healthy fertile males (control group) were included. A total of 300 semen samples were collected after 3 to 5 days of sexual abstinence. Volume, pH, concentration, normal morphology, and motility were evaluated. Samples were seeded using a calibrated loop on agar and EMB plates, which were incubated overnight. The microorganisms were identified by Gram staining technique, catalase and coagulase tests. RESULTS: The prevalence of seminal infection among infertile males in Qom was 21%. Among these infected samples 61.9%, 14.28%, 14.28% and 9.25% were contaminated with Staphylococcus aureus, coagulase negative staphylococci, Streptococcus and Escherichia coli, respectively. All the identified bacteria except Streptococcus caused a significant decrease in sperm concentration. Sperm motility was significantly lower in E. coli contaminated samples than in the control group, and the presence of E. coli and S. aureus led to a decline in normal morphology of the sperms. CONCLUSION: Sperm bacterial contamination is quite frequent and could contribute to the deterioration of the sperm quality of infertile males. Tehran University of Medical Sciences 2018-04 /pmc/articles/PMC6039451/ /pubmed/29997751 Text en Copyright© 2018 Iranian Neuroscience Society http://creativecommons.org/licenses/by/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
Berjis, Katayon
Ghiasi, Mahdieh
Sangy, Sareh
Study of seminal infection among an infertile male population in Qom, Iran, and its effect on sperm quality
title Study of seminal infection among an infertile male population in Qom, Iran, and its effect on sperm quality
title_full Study of seminal infection among an infertile male population in Qom, Iran, and its effect on sperm quality
title_fullStr Study of seminal infection among an infertile male population in Qom, Iran, and its effect on sperm quality
title_full_unstemmed Study of seminal infection among an infertile male population in Qom, Iran, and its effect on sperm quality
title_short Study of seminal infection among an infertile male population in Qom, Iran, and its effect on sperm quality
title_sort study of seminal infection among an infertile male population in qom, iran, and its effect on sperm quality
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6039451/
https://www.ncbi.nlm.nih.gov/pubmed/29997751
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