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Association of Transfer RNA Fragments in White Blood Cells With Antibody Response to Bovine Leukemia Virus in Holstein Cattle

Bovine leukemia virus (BLV) affects cattle health and productivity worldwide, causing abnormal immune function and immunosuppression. Transfer RNA fragments (tRFs) are known to be involved in inhibition of gene expression and have been associated with stress and immune response, tumor growth, and vi...

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Autores principales: Taxis, Tasia M., Kehrli, Marcus E., D’Orey-Branco, Rui, Casas, Eduardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6039543/
https://www.ncbi.nlm.nih.gov/pubmed/30023000
http://dx.doi.org/10.3389/fgene.2018.00236
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author Taxis, Tasia M.
Kehrli, Marcus E.
D’Orey-Branco, Rui
Casas, Eduardo
author_facet Taxis, Tasia M.
Kehrli, Marcus E.
D’Orey-Branco, Rui
Casas, Eduardo
author_sort Taxis, Tasia M.
collection PubMed
description Bovine leukemia virus (BLV) affects cattle health and productivity worldwide, causing abnormal immune function and immunosuppression. Transfer RNA fragments (tRFs) are known to be involved in inhibition of gene expression and have been associated with stress and immune response, tumor growth, and viral infection. The objective of this study was to identify tRFs associated with antibody response to BLV in Holstein cattle. Sera from 14 animals were collected to establish IgG reactivity to BLV by ELISA. Seven animals were seropositive (positive group) and seven were seronegative (negative group) for BLV exposure. Leukocytes from each animal were collected and tRFs were extracted for sequencing. tRF5(GlnCTG), tRF5(GlnTTG), and tRF5(HisGTG), were significantly different between seropositive and seronegative groups (P < 0.0067). In all cases the positive group had a lower number of normalized sequences for tRFs when compared to the negative group. Result suggests that tRF5s could potentially be used as biomarkers to establish exposure of cattle to BLV.
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spelling pubmed-60395432018-07-18 Association of Transfer RNA Fragments in White Blood Cells With Antibody Response to Bovine Leukemia Virus in Holstein Cattle Taxis, Tasia M. Kehrli, Marcus E. D’Orey-Branco, Rui Casas, Eduardo Front Genet Genetics Bovine leukemia virus (BLV) affects cattle health and productivity worldwide, causing abnormal immune function and immunosuppression. Transfer RNA fragments (tRFs) are known to be involved in inhibition of gene expression and have been associated with stress and immune response, tumor growth, and viral infection. The objective of this study was to identify tRFs associated with antibody response to BLV in Holstein cattle. Sera from 14 animals were collected to establish IgG reactivity to BLV by ELISA. Seven animals were seropositive (positive group) and seven were seronegative (negative group) for BLV exposure. Leukocytes from each animal were collected and tRFs were extracted for sequencing. tRF5(GlnCTG), tRF5(GlnTTG), and tRF5(HisGTG), were significantly different between seropositive and seronegative groups (P < 0.0067). In all cases the positive group had a lower number of normalized sequences for tRFs when compared to the negative group. Result suggests that tRF5s could potentially be used as biomarkers to establish exposure of cattle to BLV. Frontiers Media S.A. 2018-07-04 /pmc/articles/PMC6039543/ /pubmed/30023000 http://dx.doi.org/10.3389/fgene.2018.00236 Text en Copyright © 2018 Taxis, Kehrli, D’Orey-Branco and Casas. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Taxis, Tasia M.
Kehrli, Marcus E.
D’Orey-Branco, Rui
Casas, Eduardo
Association of Transfer RNA Fragments in White Blood Cells With Antibody Response to Bovine Leukemia Virus in Holstein Cattle
title Association of Transfer RNA Fragments in White Blood Cells With Antibody Response to Bovine Leukemia Virus in Holstein Cattle
title_full Association of Transfer RNA Fragments in White Blood Cells With Antibody Response to Bovine Leukemia Virus in Holstein Cattle
title_fullStr Association of Transfer RNA Fragments in White Blood Cells With Antibody Response to Bovine Leukemia Virus in Holstein Cattle
title_full_unstemmed Association of Transfer RNA Fragments in White Blood Cells With Antibody Response to Bovine Leukemia Virus in Holstein Cattle
title_short Association of Transfer RNA Fragments in White Blood Cells With Antibody Response to Bovine Leukemia Virus in Holstein Cattle
title_sort association of transfer rna fragments in white blood cells with antibody response to bovine leukemia virus in holstein cattle
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6039543/
https://www.ncbi.nlm.nih.gov/pubmed/30023000
http://dx.doi.org/10.3389/fgene.2018.00236
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