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Transgenerational Epigenetic Inheritance Under Environmental Stress by Genome-Wide DNA Methylation Profiling in Cyanobacterium

Epigenetic modifications such as DNA methylation are well known as connected with many important biological processes. Rapid accumulating evidence shows environmental stress can generate particular defense epigenetic changes across generations in eukaryotes. This transgenerational epigenetic inherit...

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Autores principales: Hu, Lang, Xiao, Peng, Jiang, Yongguang, Dong, Mingjie, Chen, Zixi, Li, Hui, Hu, Zhangli, Lei, Anping, Wang, Jiangxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6039552/
https://www.ncbi.nlm.nih.gov/pubmed/30022974
http://dx.doi.org/10.3389/fmicb.2018.01479
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author Hu, Lang
Xiao, Peng
Jiang, Yongguang
Dong, Mingjie
Chen, Zixi
Li, Hui
Hu, Zhangli
Lei, Anping
Wang, Jiangxin
author_facet Hu, Lang
Xiao, Peng
Jiang, Yongguang
Dong, Mingjie
Chen, Zixi
Li, Hui
Hu, Zhangli
Lei, Anping
Wang, Jiangxin
author_sort Hu, Lang
collection PubMed
description Epigenetic modifications such as DNA methylation are well known as connected with many important biological processes. Rapid accumulating evidence shows environmental stress can generate particular defense epigenetic changes across generations in eukaryotes. This transgenerational epigenetic inheritance in animals and plants has gained interest over the last years. Cyanobacteria play very crucial role in the earth, and as the primary producer they can adapt to nearly all diverse environments. However, few knowledge about the genome wide epigenetic information such as methylome information in cyanobacteria, especially under any environment stress, was reported so far. In this study we profiled the genome-wide cytosine methylation from a model cyanobacterium Synechocystis sp. PCC 6803, and explored the possibility of transgenerational epigenetic process in this ancient single-celled prokaryote by comparing the DNA methylomes among normal nitrogen medium cultivation, nitrogen starvation for 72 h and nitrogen recovery for about 12 generations. Our results shows that DNA methylation patterns in nitrogen starvation and nitrogen recovery are much more similar with each other, significantly different from that of the normal nitrogen. This study reveals the difference in global DNA methylation pattern of cyanobacteria between normal and nutrient stress conditions and reports the evidence of transgenerational epigenetic process in cyanobacteria. The results of this study may contribute to a better understanding of epigenetic regulation in prokaryotic adaptation to and survive in the ever changing environment.
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spelling pubmed-60395522018-07-18 Transgenerational Epigenetic Inheritance Under Environmental Stress by Genome-Wide DNA Methylation Profiling in Cyanobacterium Hu, Lang Xiao, Peng Jiang, Yongguang Dong, Mingjie Chen, Zixi Li, Hui Hu, Zhangli Lei, Anping Wang, Jiangxin Front Microbiol Microbiology Epigenetic modifications such as DNA methylation are well known as connected with many important biological processes. Rapid accumulating evidence shows environmental stress can generate particular defense epigenetic changes across generations in eukaryotes. This transgenerational epigenetic inheritance in animals and plants has gained interest over the last years. Cyanobacteria play very crucial role in the earth, and as the primary producer they can adapt to nearly all diverse environments. However, few knowledge about the genome wide epigenetic information such as methylome information in cyanobacteria, especially under any environment stress, was reported so far. In this study we profiled the genome-wide cytosine methylation from a model cyanobacterium Synechocystis sp. PCC 6803, and explored the possibility of transgenerational epigenetic process in this ancient single-celled prokaryote by comparing the DNA methylomes among normal nitrogen medium cultivation, nitrogen starvation for 72 h and nitrogen recovery for about 12 generations. Our results shows that DNA methylation patterns in nitrogen starvation and nitrogen recovery are much more similar with each other, significantly different from that of the normal nitrogen. This study reveals the difference in global DNA methylation pattern of cyanobacteria between normal and nutrient stress conditions and reports the evidence of transgenerational epigenetic process in cyanobacteria. The results of this study may contribute to a better understanding of epigenetic regulation in prokaryotic adaptation to and survive in the ever changing environment. Frontiers Media S.A. 2018-07-04 /pmc/articles/PMC6039552/ /pubmed/30022974 http://dx.doi.org/10.3389/fmicb.2018.01479 Text en Copyright © 2018 Hu, Xiao, Jiang, Dong, Chen, Li, Hu, Lei and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Hu, Lang
Xiao, Peng
Jiang, Yongguang
Dong, Mingjie
Chen, Zixi
Li, Hui
Hu, Zhangli
Lei, Anping
Wang, Jiangxin
Transgenerational Epigenetic Inheritance Under Environmental Stress by Genome-Wide DNA Methylation Profiling in Cyanobacterium
title Transgenerational Epigenetic Inheritance Under Environmental Stress by Genome-Wide DNA Methylation Profiling in Cyanobacterium
title_full Transgenerational Epigenetic Inheritance Under Environmental Stress by Genome-Wide DNA Methylation Profiling in Cyanobacterium
title_fullStr Transgenerational Epigenetic Inheritance Under Environmental Stress by Genome-Wide DNA Methylation Profiling in Cyanobacterium
title_full_unstemmed Transgenerational Epigenetic Inheritance Under Environmental Stress by Genome-Wide DNA Methylation Profiling in Cyanobacterium
title_short Transgenerational Epigenetic Inheritance Under Environmental Stress by Genome-Wide DNA Methylation Profiling in Cyanobacterium
title_sort transgenerational epigenetic inheritance under environmental stress by genome-wide dna methylation profiling in cyanobacterium
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6039552/
https://www.ncbi.nlm.nih.gov/pubmed/30022974
http://dx.doi.org/10.3389/fmicb.2018.01479
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