Cargando…

Profiling of lung microbiota in the patients with obstructive sleep apnea

Lung microbiota may affect innate immunity and treatment consequence in the obstructive sleep apnea (OSA) patients. Bronchoalveolar lavage fluid (BALF) was obtained from 11 OSA patients and 8 patients with other lung diseases as control, and used for lung microbiota profiling by PCR amplification an...

Descripción completa

Detalles Bibliográficos
Autores principales: Lu, Dongmei, Yao, Xiaoguang, Abulimiti, Ayinigeer, Cai, Li, Zhou, Ling, Hong, Jing, Li, Nanfang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer Health 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6039595/
https://www.ncbi.nlm.nih.gov/pubmed/29952967
http://dx.doi.org/10.1097/MD.0000000000011175
_version_ 1783338706563760128
author Lu, Dongmei
Yao, Xiaoguang
Abulimiti, Ayinigeer
Cai, Li
Zhou, Ling
Hong, Jing
Li, Nanfang
author_facet Lu, Dongmei
Yao, Xiaoguang
Abulimiti, Ayinigeer
Cai, Li
Zhou, Ling
Hong, Jing
Li, Nanfang
author_sort Lu, Dongmei
collection PubMed
description Lung microbiota may affect innate immunity and treatment consequence in the obstructive sleep apnea (OSA) patients. Bronchoalveolar lavage fluid (BALF) was obtained from 11 OSA patients and 8 patients with other lung diseases as control, and used for lung microbiota profiling by PCR amplification and sequencing of the microbial samples. It was demonstrated that phyla of Firmicutes, Fusobacteria, and Bacteriodetes were relatively abundant in the lung microbiota. Alpha-diversity comparison between OSA and control group revealed that Proteobacteria and Fusobacteria were significantly higher in OSA patients (0.3863 ± 0.0631 and 0.0682 ± 0.0159, respectively) than that in control group (0.119 ± 0.074 and 0.0006 ± 0.0187, respectively, P < .05 for both phyla). In contrast, Firmicutes was significantly less in OSA patients (0.1371 ± 0.0394) compared with that in the control group (0.384 ± 0.046, P < .05). Comparison within a group (ß-diversity) indicated that the top 5 phyla in the OSA lung were Proteobacteria, Bacteroidetes, Firmicutes, Fusobacteria, and Acidobacteria, while the top 5 phyla in the control group were Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, and Acidobacteria. These findings indicated that lung microbiota in OSA is distinct from that of non-OSA patients. Manipulation of the microbiota may be an alternative strategy to augment airway immunity and to reduce susceptibility to airway infection.
format Online
Article
Text
id pubmed-6039595
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Wolters Kluwer Health
record_format MEDLINE/PubMed
spelling pubmed-60395952018-07-16 Profiling of lung microbiota in the patients with obstructive sleep apnea Lu, Dongmei Yao, Xiaoguang Abulimiti, Ayinigeer Cai, Li Zhou, Ling Hong, Jing Li, Nanfang Medicine (Baltimore) Research Article Lung microbiota may affect innate immunity and treatment consequence in the obstructive sleep apnea (OSA) patients. Bronchoalveolar lavage fluid (BALF) was obtained from 11 OSA patients and 8 patients with other lung diseases as control, and used for lung microbiota profiling by PCR amplification and sequencing of the microbial samples. It was demonstrated that phyla of Firmicutes, Fusobacteria, and Bacteriodetes were relatively abundant in the lung microbiota. Alpha-diversity comparison between OSA and control group revealed that Proteobacteria and Fusobacteria were significantly higher in OSA patients (0.3863 ± 0.0631 and 0.0682 ± 0.0159, respectively) than that in control group (0.119 ± 0.074 and 0.0006 ± 0.0187, respectively, P < .05 for both phyla). In contrast, Firmicutes was significantly less in OSA patients (0.1371 ± 0.0394) compared with that in the control group (0.384 ± 0.046, P < .05). Comparison within a group (ß-diversity) indicated that the top 5 phyla in the OSA lung were Proteobacteria, Bacteroidetes, Firmicutes, Fusobacteria, and Acidobacteria, while the top 5 phyla in the control group were Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, and Acidobacteria. These findings indicated that lung microbiota in OSA is distinct from that of non-OSA patients. Manipulation of the microbiota may be an alternative strategy to augment airway immunity and to reduce susceptibility to airway infection. Wolters Kluwer Health 2018-06-29 /pmc/articles/PMC6039595/ /pubmed/29952967 http://dx.doi.org/10.1097/MD.0000000000011175 Text en Copyright © 2018 the Author(s). Published by Wolters Kluwer Health, Inc. http://creativecommons.org/licenses/by-nd/4.0 This is an open access article distributed under the Creative Commons Attribution-NoDerivatives License 4.0, which allows for redistribution, commercial and non-commercial, as long as it is passed along unchanged and in whole, with credit to the author. http://creativecommons.org/licenses/by-nd/4.0
spellingShingle Research Article
Lu, Dongmei
Yao, Xiaoguang
Abulimiti, Ayinigeer
Cai, Li
Zhou, Ling
Hong, Jing
Li, Nanfang
Profiling of lung microbiota in the patients with obstructive sleep apnea
title Profiling of lung microbiota in the patients with obstructive sleep apnea
title_full Profiling of lung microbiota in the patients with obstructive sleep apnea
title_fullStr Profiling of lung microbiota in the patients with obstructive sleep apnea
title_full_unstemmed Profiling of lung microbiota in the patients with obstructive sleep apnea
title_short Profiling of lung microbiota in the patients with obstructive sleep apnea
title_sort profiling of lung microbiota in the patients with obstructive sleep apnea
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6039595/
https://www.ncbi.nlm.nih.gov/pubmed/29952967
http://dx.doi.org/10.1097/MD.0000000000011175
work_keys_str_mv AT ludongmei profilingoflungmicrobiotainthepatientswithobstructivesleepapnea
AT yaoxiaoguang profilingoflungmicrobiotainthepatientswithobstructivesleepapnea
AT abulimitiayinigeer profilingoflungmicrobiotainthepatientswithobstructivesleepapnea
AT caili profilingoflungmicrobiotainthepatientswithobstructivesleepapnea
AT zhouling profilingoflungmicrobiotainthepatientswithobstructivesleepapnea
AT hongjing profilingoflungmicrobiotainthepatientswithobstructivesleepapnea
AT linanfang profilingoflungmicrobiotainthepatientswithobstructivesleepapnea