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Isolation and characterization of two phosphate-solubilizing fungi from rhizosphere soil of moso bamboo and their functional capacities when exposed to different phosphorus sources and pH environments
Phosphate-solubilizing fungi (PSF) generally enhance available phosphorus (P) released from soil, which contributes to plants’ P requirement, especially in P-limiting regions. In this study, two PSF, TalA-JX04 and AspN-JX16, were isolated from the rhizosphere soil of moso bamboo (Phyllostachys eduli...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6040707/ https://www.ncbi.nlm.nih.gov/pubmed/29995910 http://dx.doi.org/10.1371/journal.pone.0199625 |
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author | Zhang, Yang Chen, Fu-Sheng Wu, Xiao-Qin Luan, Feng-Gang Zhang, Lin-Ping Fang, Xiang-Min Wan, Song-Ze Hu, Xiao-Fei Ye, Jian-Ren |
author_facet | Zhang, Yang Chen, Fu-Sheng Wu, Xiao-Qin Luan, Feng-Gang Zhang, Lin-Ping Fang, Xiang-Min Wan, Song-Ze Hu, Xiao-Fei Ye, Jian-Ren |
author_sort | Zhang, Yang |
collection | PubMed |
description | Phosphate-solubilizing fungi (PSF) generally enhance available phosphorus (P) released from soil, which contributes to plants’ P requirement, especially in P-limiting regions. In this study, two PSF, TalA-JX04 and AspN-JX16, were isolated from the rhizosphere soil of moso bamboo (Phyllostachys edulis) widely distributed in P-deficient areas in China and identified as Talaromyces aurantiacus and Aspergillus neoniger, respectively. The two PSF were cultured in potato dextrose liquid medium with six types of initial pH values ranging from 6.5 to 1.5 to assess acid resistance. Both PSF were incubated in Pikovskaya’s liquid media with different pH values containing five recalcitrant P sources, including Ca(3)(PO(4))(2), FePO(4), CaHPO(4), AlPO(4,) and C(6)H(6)Ca(6)O(24)P(6), to estimate their P-solubilizing capacity. No significant differences were found in the biomass of both fungi grown in media with different initial pH, indicating that these fungi could grow well under acid stress. The P-solubilizing capacity of TalA-JX04 was highest in medium containing CaHPO(4), followed by Ca(3)(PO(4))(2), FePO(4), C(6)H(6)Ca(6)O(24)P(6), and AlPO(4) in six types of initial pH treatments, while the recalcitrant P-solubilizing capacity of AspN-JX16 varied with initial pH. Meanwhile, the P-solubilizing capacity of AspN-JX16 was much higher than TalA-JX04. The pH of fermentation broth was negatively correlated with P-solubilizing capacity (p<0.01), suggesting that the fungi promote the dissolution of P sources by secreting organic acids. Our results showed that TalA-JX04 and AspN-JX16 could survive in acidic environments and both fungi had a considerable ability to release soluble P by decomposing recalcitrant P-bearing compounds. The two fungi had potential for application as environment-friendly biofertilizers in subtropical bamboo ecosystem. |
format | Online Article Text |
id | pubmed-6040707 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-60407072018-07-19 Isolation and characterization of two phosphate-solubilizing fungi from rhizosphere soil of moso bamboo and their functional capacities when exposed to different phosphorus sources and pH environments Zhang, Yang Chen, Fu-Sheng Wu, Xiao-Qin Luan, Feng-Gang Zhang, Lin-Ping Fang, Xiang-Min Wan, Song-Ze Hu, Xiao-Fei Ye, Jian-Ren PLoS One Research Article Phosphate-solubilizing fungi (PSF) generally enhance available phosphorus (P) released from soil, which contributes to plants’ P requirement, especially in P-limiting regions. In this study, two PSF, TalA-JX04 and AspN-JX16, were isolated from the rhizosphere soil of moso bamboo (Phyllostachys edulis) widely distributed in P-deficient areas in China and identified as Talaromyces aurantiacus and Aspergillus neoniger, respectively. The two PSF were cultured in potato dextrose liquid medium with six types of initial pH values ranging from 6.5 to 1.5 to assess acid resistance. Both PSF were incubated in Pikovskaya’s liquid media with different pH values containing five recalcitrant P sources, including Ca(3)(PO(4))(2), FePO(4), CaHPO(4), AlPO(4,) and C(6)H(6)Ca(6)O(24)P(6), to estimate their P-solubilizing capacity. No significant differences were found in the biomass of both fungi grown in media with different initial pH, indicating that these fungi could grow well under acid stress. The P-solubilizing capacity of TalA-JX04 was highest in medium containing CaHPO(4), followed by Ca(3)(PO(4))(2), FePO(4), C(6)H(6)Ca(6)O(24)P(6), and AlPO(4) in six types of initial pH treatments, while the recalcitrant P-solubilizing capacity of AspN-JX16 varied with initial pH. Meanwhile, the P-solubilizing capacity of AspN-JX16 was much higher than TalA-JX04. The pH of fermentation broth was negatively correlated with P-solubilizing capacity (p<0.01), suggesting that the fungi promote the dissolution of P sources by secreting organic acids. Our results showed that TalA-JX04 and AspN-JX16 could survive in acidic environments and both fungi had a considerable ability to release soluble P by decomposing recalcitrant P-bearing compounds. The two fungi had potential for application as environment-friendly biofertilizers in subtropical bamboo ecosystem. Public Library of Science 2018-07-11 /pmc/articles/PMC6040707/ /pubmed/29995910 http://dx.doi.org/10.1371/journal.pone.0199625 Text en © 2018 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Zhang, Yang Chen, Fu-Sheng Wu, Xiao-Qin Luan, Feng-Gang Zhang, Lin-Ping Fang, Xiang-Min Wan, Song-Ze Hu, Xiao-Fei Ye, Jian-Ren Isolation and characterization of two phosphate-solubilizing fungi from rhizosphere soil of moso bamboo and their functional capacities when exposed to different phosphorus sources and pH environments |
title | Isolation and characterization of two phosphate-solubilizing fungi from rhizosphere soil of moso bamboo and their functional capacities when exposed to different phosphorus sources and pH environments |
title_full | Isolation and characterization of two phosphate-solubilizing fungi from rhizosphere soil of moso bamboo and their functional capacities when exposed to different phosphorus sources and pH environments |
title_fullStr | Isolation and characterization of two phosphate-solubilizing fungi from rhizosphere soil of moso bamboo and their functional capacities when exposed to different phosphorus sources and pH environments |
title_full_unstemmed | Isolation and characterization of two phosphate-solubilizing fungi from rhizosphere soil of moso bamboo and their functional capacities when exposed to different phosphorus sources and pH environments |
title_short | Isolation and characterization of two phosphate-solubilizing fungi from rhizosphere soil of moso bamboo and their functional capacities when exposed to different phosphorus sources and pH environments |
title_sort | isolation and characterization of two phosphate-solubilizing fungi from rhizosphere soil of moso bamboo and their functional capacities when exposed to different phosphorus sources and ph environments |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6040707/ https://www.ncbi.nlm.nih.gov/pubmed/29995910 http://dx.doi.org/10.1371/journal.pone.0199625 |
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