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Improving preservation state assessment of carbonate microfossils in paleontological research using label-free stimulated Raman imaging

In micropaleontological and paleoclimatological studies based on microfossil morphology and geochemistry, assessing the preservation state of fossils is of the highest importance, as diagenetic alteration invalidates textural features and compromises the correct interpretation of stable isotope and...

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Autores principales: Golreihan, Asefeh, Steuwe, Christian, Woelders, Lineke, Deprez, Arne, Fujita, Yasuhiko, Vellekoop, Johan, Swennen, Rudy, Roeffaers, Maarten B. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6040746/
https://www.ncbi.nlm.nih.gov/pubmed/29995961
http://dx.doi.org/10.1371/journal.pone.0199695
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author Golreihan, Asefeh
Steuwe, Christian
Woelders, Lineke
Deprez, Arne
Fujita, Yasuhiko
Vellekoop, Johan
Swennen, Rudy
Roeffaers, Maarten B. J.
author_facet Golreihan, Asefeh
Steuwe, Christian
Woelders, Lineke
Deprez, Arne
Fujita, Yasuhiko
Vellekoop, Johan
Swennen, Rudy
Roeffaers, Maarten B. J.
author_sort Golreihan, Asefeh
collection PubMed
description In micropaleontological and paleoclimatological studies based on microfossil morphology and geochemistry, assessing the preservation state of fossils is of the highest importance, as diagenetic alteration invalidates textural features and compromises the correct interpretation of stable isotope and trace elemental analysis. In this paper, we present a novel non-invasive and label-free tomographic approach to reconstruct the three-dimensional architecture of microfossils with submicron resolution based on stimulated Raman scattering (SRS). Furthermore, this technique allows deciphering the three-dimensional (3D) distribution of the minerals within these fossils in a chemically sensitive manner. Our method, therefore, allows to identify microfossils, to chemically map their internal structure and eventually to determine their preservation state. We demonstrate the effectiveness of this method by analyzing several benthic and planktonic foraminifera, obtaining full 3D distributions of carbonate, iron oxide and porosity for each specimen. Subsequently, the preservation state of each microfossil can be assessed using these 3D compositional maps. The technique is highly sensitive, non-destructive, time-efficient and avoids the need for sample pretreatment. Therefore, its predestined application is the final check of the state of microfossils before applying subsequent geochemical analyses.
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spelling pubmed-60407462018-07-19 Improving preservation state assessment of carbonate microfossils in paleontological research using label-free stimulated Raman imaging Golreihan, Asefeh Steuwe, Christian Woelders, Lineke Deprez, Arne Fujita, Yasuhiko Vellekoop, Johan Swennen, Rudy Roeffaers, Maarten B. J. PLoS One Research Article In micropaleontological and paleoclimatological studies based on microfossil morphology and geochemistry, assessing the preservation state of fossils is of the highest importance, as diagenetic alteration invalidates textural features and compromises the correct interpretation of stable isotope and trace elemental analysis. In this paper, we present a novel non-invasive and label-free tomographic approach to reconstruct the three-dimensional architecture of microfossils with submicron resolution based on stimulated Raman scattering (SRS). Furthermore, this technique allows deciphering the three-dimensional (3D) distribution of the minerals within these fossils in a chemically sensitive manner. Our method, therefore, allows to identify microfossils, to chemically map their internal structure and eventually to determine their preservation state. We demonstrate the effectiveness of this method by analyzing several benthic and planktonic foraminifera, obtaining full 3D distributions of carbonate, iron oxide and porosity for each specimen. Subsequently, the preservation state of each microfossil can be assessed using these 3D compositional maps. The technique is highly sensitive, non-destructive, time-efficient and avoids the need for sample pretreatment. Therefore, its predestined application is the final check of the state of microfossils before applying subsequent geochemical analyses. Public Library of Science 2018-07-11 /pmc/articles/PMC6040746/ /pubmed/29995961 http://dx.doi.org/10.1371/journal.pone.0199695 Text en © 2018 Golreihan et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Golreihan, Asefeh
Steuwe, Christian
Woelders, Lineke
Deprez, Arne
Fujita, Yasuhiko
Vellekoop, Johan
Swennen, Rudy
Roeffaers, Maarten B. J.
Improving preservation state assessment of carbonate microfossils in paleontological research using label-free stimulated Raman imaging
title Improving preservation state assessment of carbonate microfossils in paleontological research using label-free stimulated Raman imaging
title_full Improving preservation state assessment of carbonate microfossils in paleontological research using label-free stimulated Raman imaging
title_fullStr Improving preservation state assessment of carbonate microfossils in paleontological research using label-free stimulated Raman imaging
title_full_unstemmed Improving preservation state assessment of carbonate microfossils in paleontological research using label-free stimulated Raman imaging
title_short Improving preservation state assessment of carbonate microfossils in paleontological research using label-free stimulated Raman imaging
title_sort improving preservation state assessment of carbonate microfossils in paleontological research using label-free stimulated raman imaging
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6040746/
https://www.ncbi.nlm.nih.gov/pubmed/29995961
http://dx.doi.org/10.1371/journal.pone.0199695
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