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All-Optical Assay to Study Biological Neural Networks

We introduce a novel all-optical assay for functional studies of biological neural networks in vitro. We created a novel optogenetic construct named OptoCaMP which is a combination of a channelrhodopsin variant (CheRiff) and a red genetically encoded calcium indicator (GECI) (jRCaMP1b). It enables s...

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Detalles Bibliográficos
Autores principales: Afshar Saber, Wardiya, Gasparoli, Federico M., Dirks, Marjet G., Gunn-Moore, Frank J., Antkowiak, Maciej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6041400/
https://www.ncbi.nlm.nih.gov/pubmed/30026684
http://dx.doi.org/10.3389/fnins.2018.00451
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author Afshar Saber, Wardiya
Gasparoli, Federico M.
Dirks, Marjet G.
Gunn-Moore, Frank J.
Antkowiak, Maciej
author_facet Afshar Saber, Wardiya
Gasparoli, Federico M.
Dirks, Marjet G.
Gunn-Moore, Frank J.
Antkowiak, Maciej
author_sort Afshar Saber, Wardiya
collection PubMed
description We introduce a novel all-optical assay for functional studies of biological neural networks in vitro. We created a novel optogenetic construct named OptoCaMP which is a combination of a channelrhodopsin variant (CheRiff) and a red genetically encoded calcium indicator (GECI) (jRCaMP1b). It enables simultaneous optical stimulation and recording from large population of neurons with single-cell readout. Additionally, we have developed a spatio-temporal all-optical assay to simultaneously stimulate a sub-section of a neural network and record evoked calcium activity, in both stimulated and non-stimulated neurons, thus allowing the investigation of the spread of excitation through an interconnected network. Finally, we demonstrate the sensitivity of this assay to the change of neural network connectivity.
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spelling pubmed-60414002018-07-19 All-Optical Assay to Study Biological Neural Networks Afshar Saber, Wardiya Gasparoli, Federico M. Dirks, Marjet G. Gunn-Moore, Frank J. Antkowiak, Maciej Front Neurosci Neuroscience We introduce a novel all-optical assay for functional studies of biological neural networks in vitro. We created a novel optogenetic construct named OptoCaMP which is a combination of a channelrhodopsin variant (CheRiff) and a red genetically encoded calcium indicator (GECI) (jRCaMP1b). It enables simultaneous optical stimulation and recording from large population of neurons with single-cell readout. Additionally, we have developed a spatio-temporal all-optical assay to simultaneously stimulate a sub-section of a neural network and record evoked calcium activity, in both stimulated and non-stimulated neurons, thus allowing the investigation of the spread of excitation through an interconnected network. Finally, we demonstrate the sensitivity of this assay to the change of neural network connectivity. Frontiers Media S.A. 2018-07-05 /pmc/articles/PMC6041400/ /pubmed/30026684 http://dx.doi.org/10.3389/fnins.2018.00451 Text en Copyright © 2018 Afshar Saber, Gasparoli, Dirks, Gunn-Moore and Antkowiak. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Afshar Saber, Wardiya
Gasparoli, Federico M.
Dirks, Marjet G.
Gunn-Moore, Frank J.
Antkowiak, Maciej
All-Optical Assay to Study Biological Neural Networks
title All-Optical Assay to Study Biological Neural Networks
title_full All-Optical Assay to Study Biological Neural Networks
title_fullStr All-Optical Assay to Study Biological Neural Networks
title_full_unstemmed All-Optical Assay to Study Biological Neural Networks
title_short All-Optical Assay to Study Biological Neural Networks
title_sort all-optical assay to study biological neural networks
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6041400/
https://www.ncbi.nlm.nih.gov/pubmed/30026684
http://dx.doi.org/10.3389/fnins.2018.00451
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