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Rapid detection of four non-fermenting Gram-negative bacteria directly from cystic fibrosis patient's respiratory samples on the BD MAX™ system
The aim of this study was to develop a multiplex PCR test to detect Achromobacter xylosoxidans (AX), Burkholderia cepacia (BC), Pseudomonas aeruginosa (PSA) and Stenotrophomonas maltophilia (SM) directly from CF patient's respiratory samples using the open mode of the BD MAX™ System. A total of...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6041425/ https://www.ncbi.nlm.nih.gov/pubmed/30009245 http://dx.doi.org/10.1016/j.plabm.2018.e00102 |
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author | Rocchetti, Talita T. Silbert, Suzane Gostnell, Alicia Kubasek, Carly Jerris, Robert Vong, Jennifer Widen, Raymond |
author_facet | Rocchetti, Talita T. Silbert, Suzane Gostnell, Alicia Kubasek, Carly Jerris, Robert Vong, Jennifer Widen, Raymond |
author_sort | Rocchetti, Talita T. |
collection | PubMed |
description | The aim of this study was to develop a multiplex PCR test to detect Achromobacter xylosoxidans (AX), Burkholderia cepacia (BC), Pseudomonas aeruginosa (PSA) and Stenotrophomonas maltophilia (SM) directly from CF patient's respiratory samples using the open mode of the BD MAX™ System. A total of 402 CF respiratory samples were evaluated by culture and PCR. Specific sets of primers and probes for each target were designed in-house. Out of 402 samples tested, 196 were identified as negative and 206 as positive by culture for AX, PSA, BC and SM. Among culture positive samples, PCR detected 21/27 AX, 4/5 BC, 138/140 PSA and 29/34 SM. In addition, PCR assay identified 35 samples as positive that were initially negative by culture for those 4 targets. The CF BDM test proved to be an excellent tool to detect AX, BC, PSA and SM by real-time PCR on an automated platform. |
format | Online Article Text |
id | pubmed-6041425 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-60414252018-07-13 Rapid detection of four non-fermenting Gram-negative bacteria directly from cystic fibrosis patient's respiratory samples on the BD MAX™ system Rocchetti, Talita T. Silbert, Suzane Gostnell, Alicia Kubasek, Carly Jerris, Robert Vong, Jennifer Widen, Raymond Pract Lab Med Article The aim of this study was to develop a multiplex PCR test to detect Achromobacter xylosoxidans (AX), Burkholderia cepacia (BC), Pseudomonas aeruginosa (PSA) and Stenotrophomonas maltophilia (SM) directly from CF patient's respiratory samples using the open mode of the BD MAX™ System. A total of 402 CF respiratory samples were evaluated by culture and PCR. Specific sets of primers and probes for each target were designed in-house. Out of 402 samples tested, 196 were identified as negative and 206 as positive by culture for AX, PSA, BC and SM. Among culture positive samples, PCR detected 21/27 AX, 4/5 BC, 138/140 PSA and 29/34 SM. In addition, PCR assay identified 35 samples as positive that were initially negative by culture for those 4 targets. The CF BDM test proved to be an excellent tool to detect AX, BC, PSA and SM by real-time PCR on an automated platform. Elsevier 2018-05-24 /pmc/articles/PMC6041425/ /pubmed/30009245 http://dx.doi.org/10.1016/j.plabm.2018.e00102 Text en © 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Rocchetti, Talita T. Silbert, Suzane Gostnell, Alicia Kubasek, Carly Jerris, Robert Vong, Jennifer Widen, Raymond Rapid detection of four non-fermenting Gram-negative bacteria directly from cystic fibrosis patient's respiratory samples on the BD MAX™ system |
title | Rapid detection of four non-fermenting Gram-negative bacteria directly from cystic fibrosis patient's respiratory samples on the BD MAX™ system |
title_full | Rapid detection of four non-fermenting Gram-negative bacteria directly from cystic fibrosis patient's respiratory samples on the BD MAX™ system |
title_fullStr | Rapid detection of four non-fermenting Gram-negative bacteria directly from cystic fibrosis patient's respiratory samples on the BD MAX™ system |
title_full_unstemmed | Rapid detection of four non-fermenting Gram-negative bacteria directly from cystic fibrosis patient's respiratory samples on the BD MAX™ system |
title_short | Rapid detection of four non-fermenting Gram-negative bacteria directly from cystic fibrosis patient's respiratory samples on the BD MAX™ system |
title_sort | rapid detection of four non-fermenting gram-negative bacteria directly from cystic fibrosis patient's respiratory samples on the bd max™ system |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6041425/ https://www.ncbi.nlm.nih.gov/pubmed/30009245 http://dx.doi.org/10.1016/j.plabm.2018.e00102 |
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