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WRKY1 acts as a key component improving resistance against Alternaria solani in wild tomato, Solanum arcanum Peralta
Early blight (EB), caused by Alternaria solani, is a major threat to global tomato production. In comparison with cultivated tomato (Solanum lycopersicum), a wild relative, S. arcanum exhibits strong resistance against EB. However, molecular cascades operating during EB resistance in wild or cultiva...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6041441/ https://www.ncbi.nlm.nih.gov/pubmed/29377467 http://dx.doi.org/10.1111/pbi.12892 |
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author | Shinde, Balkrishna A. Dholakia, Bhushan B. Hussain, Khalid Aharoni, Asaph Giri, Ashok P. Kamble, Avinash C. |
author_facet | Shinde, Balkrishna A. Dholakia, Bhushan B. Hussain, Khalid Aharoni, Asaph Giri, Ashok P. Kamble, Avinash C. |
author_sort | Shinde, Balkrishna A. |
collection | PubMed |
description | Early blight (EB), caused by Alternaria solani, is a major threat to global tomato production. In comparison with cultivated tomato (Solanum lycopersicum), a wild relative, S. arcanum exhibits strong resistance against EB. However, molecular cascades operating during EB resistance in wild or cultivated tomato plants are largely obscure. Here, we provide novel insight into spatio‐temporal molecular events in S. arcanum against A. solani. Transcriptome and co‐expression analysis presented 33‐WRKYs as promising candidates of which 12 SaWRKYs displayed differential expression patterns in resistant and susceptible accessions during EB disease progression. Among these, SaWRKY1 exhibited induced expression with significant modulation in xyloglucan endotrans hydrolase 5 (XTH5) and MYB2 expressions that correlated with the disease phenotypes. Electro‐mobility shift assay confirmed physical interaction of recombinant SaWRKY1 to SaXTH5 and SaMYB2 promoters. Comparative WRKY1 promoter analysis between resistant and susceptible plants revealed the presence of crucial motifs for defence mechanism exclusively in resistant accession. Additionally, many defence‐related genes displayed significant expression variations in both the accessions. Further, WRKY1 overexpressing transgenic plants exhibited higher levels of EB resistance while RNAi silencing lines had increased susceptibility to A. solani with altered expression of XTH5 and MYB2. Overall, these findings demonstrate the positive influence of WRKY1 in improving EB resistance in wild tomato and this could be further utilized as a potential target through genetic engineering to augment protection against A. solani in crop plants. |
format | Online Article Text |
id | pubmed-6041441 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-60414412018-07-15 WRKY1 acts as a key component improving resistance against Alternaria solani in wild tomato, Solanum arcanum Peralta Shinde, Balkrishna A. Dholakia, Bhushan B. Hussain, Khalid Aharoni, Asaph Giri, Ashok P. Kamble, Avinash C. Plant Biotechnol J Research Articles Early blight (EB), caused by Alternaria solani, is a major threat to global tomato production. In comparison with cultivated tomato (Solanum lycopersicum), a wild relative, S. arcanum exhibits strong resistance against EB. However, molecular cascades operating during EB resistance in wild or cultivated tomato plants are largely obscure. Here, we provide novel insight into spatio‐temporal molecular events in S. arcanum against A. solani. Transcriptome and co‐expression analysis presented 33‐WRKYs as promising candidates of which 12 SaWRKYs displayed differential expression patterns in resistant and susceptible accessions during EB disease progression. Among these, SaWRKY1 exhibited induced expression with significant modulation in xyloglucan endotrans hydrolase 5 (XTH5) and MYB2 expressions that correlated with the disease phenotypes. Electro‐mobility shift assay confirmed physical interaction of recombinant SaWRKY1 to SaXTH5 and SaMYB2 promoters. Comparative WRKY1 promoter analysis between resistant and susceptible plants revealed the presence of crucial motifs for defence mechanism exclusively in resistant accession. Additionally, many defence‐related genes displayed significant expression variations in both the accessions. Further, WRKY1 overexpressing transgenic plants exhibited higher levels of EB resistance while RNAi silencing lines had increased susceptibility to A. solani with altered expression of XTH5 and MYB2. Overall, these findings demonstrate the positive influence of WRKY1 in improving EB resistance in wild tomato and this could be further utilized as a potential target through genetic engineering to augment protection against A. solani in crop plants. John Wiley and Sons Inc. 2018-05-24 2018-08 /pmc/articles/PMC6041441/ /pubmed/29377467 http://dx.doi.org/10.1111/pbi.12892 Text en © 2018 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Shinde, Balkrishna A. Dholakia, Bhushan B. Hussain, Khalid Aharoni, Asaph Giri, Ashok P. Kamble, Avinash C. WRKY1 acts as a key component improving resistance against Alternaria solani in wild tomato, Solanum arcanum Peralta |
title |
WRKY1 acts as a key component improving resistance against Alternaria solani in wild tomato, Solanum arcanum Peralta |
title_full |
WRKY1 acts as a key component improving resistance against Alternaria solani in wild tomato, Solanum arcanum Peralta |
title_fullStr |
WRKY1 acts as a key component improving resistance against Alternaria solani in wild tomato, Solanum arcanum Peralta |
title_full_unstemmed |
WRKY1 acts as a key component improving resistance against Alternaria solani in wild tomato, Solanum arcanum Peralta |
title_short |
WRKY1 acts as a key component improving resistance against Alternaria solani in wild tomato, Solanum arcanum Peralta |
title_sort | wrky1 acts as a key component improving resistance against alternaria solani in wild tomato, solanum arcanum peralta |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6041441/ https://www.ncbi.nlm.nih.gov/pubmed/29377467 http://dx.doi.org/10.1111/pbi.12892 |
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