CRISPR/Cascade 9-Mediated Genome Editing-Challenges and Opportunities

Clustered Regularly Interspaced Palindromic Repeats (CRISPR) and Cascade 9 (also known as Cas9, CRISPR associated protein 9) confer protection against invading viruses or plasmids. The CRISPR/Cascade 9 system constitutes one of the most powerful genome technologies available to researchers today. So...

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Autores principales: Roy, Bhaskar, Zhao, Jing, Yang, Chao, Luo, Wen, Xiong, Teng, Li, Yong, Fang, Xiaodong, Gao, Guanjun, Singh, Chabungbam O., Madsen, Lise, Zhou, Yong, Kristiansen, Karsten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Genetics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6042012/
https://www.ncbi.nlm.nih.gov/pubmed/30026755
http://dx.doi.org/10.3389/fgene.2018.00240
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author Roy, Bhaskar
Zhao, Jing
Yang, Chao
Luo, Wen
Xiong, Teng
Li, Yong
Fang, Xiaodong
Gao, Guanjun
Singh, Chabungbam O.
Madsen, Lise
Zhou, Yong
Kristiansen, Karsten
author_facet Roy, Bhaskar
Zhao, Jing
Yang, Chao
Luo, Wen
Xiong, Teng
Li, Yong
Fang, Xiaodong
Gao, Guanjun
Singh, Chabungbam O.
Madsen, Lise
Zhou, Yong
Kristiansen, Karsten
author_sort Roy, Bhaskar
collection PubMed
description Clustered Regularly Interspaced Palindromic Repeats (CRISPR) and Cascade 9 (also known as Cas9, CRISPR associated protein 9) confer protection against invading viruses or plasmids. The CRISPR/Cascade 9 system constitutes one of the most powerful genome technologies available to researchers today. So far, this technology has enabled efficient genome editing and modification in several model organisms and has successfully been used in biomedicine and biomedical engineering. However, challenges for efficient and safe genetic manipulation in several organisms persist. Here, we review functional approaches and future challenges associated with the use of the CRISPR/Cascade 9 genome editing system and discuss opportunities, ethical issues and future directions within this field.
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spelling pubmed-60420122018-07-19 CRISPR/Cascade 9-Mediated Genome Editing-Challenges and Opportunities Roy, Bhaskar Zhao, Jing Yang, Chao Luo, Wen Xiong, Teng Li, Yong Fang, Xiaodong Gao, Guanjun Singh, Chabungbam O. Madsen, Lise Zhou, Yong Kristiansen, Karsten Front Genet Genetics Clustered Regularly Interspaced Palindromic Repeats (CRISPR) and Cascade 9 (also known as Cas9, CRISPR associated protein 9) confer protection against invading viruses or plasmids. The CRISPR/Cascade 9 system constitutes one of the most powerful genome technologies available to researchers today. So far, this technology has enabled efficient genome editing and modification in several model organisms and has successfully been used in biomedicine and biomedical engineering. However, challenges for efficient and safe genetic manipulation in several organisms persist. Here, we review functional approaches and future challenges associated with the use of the CRISPR/Cascade 9 genome editing system and discuss opportunities, ethical issues and future directions within this field. Frontiers Media S.A. 2018-07-05 /pmc/articles/PMC6042012/ /pubmed/30026755 http://dx.doi.org/10.3389/fgene.2018.00240 Text en Copyright © 2018 Roy, Zhao, Yang, Luo, Xiong, Li, Fang, Gao, Singh, Madsen, Zhou and Kristiansen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Roy, Bhaskar
Zhao, Jing
Yang, Chao
Luo, Wen
Xiong, Teng
Li, Yong
Fang, Xiaodong
Gao, Guanjun
Singh, Chabungbam O.
Madsen, Lise
Zhou, Yong
Kristiansen, Karsten
CRISPR/Cascade 9-Mediated Genome Editing-Challenges and Opportunities
title CRISPR/Cascade 9-Mediated Genome Editing-Challenges and Opportunities
title_full CRISPR/Cascade 9-Mediated Genome Editing-Challenges and Opportunities
title_fullStr CRISPR/Cascade 9-Mediated Genome Editing-Challenges and Opportunities
title_full_unstemmed CRISPR/Cascade 9-Mediated Genome Editing-Challenges and Opportunities
title_short CRISPR/Cascade 9-Mediated Genome Editing-Challenges and Opportunities
title_sort crispr/cascade 9-mediated genome editing-challenges and opportunities
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6042012/
https://www.ncbi.nlm.nih.gov/pubmed/30026755
http://dx.doi.org/10.3389/fgene.2018.00240
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