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An archaeal primase functions as a nanoscale caliper to define primer length

The cellular replicative DNA polymerases cannot initiate DNA synthesis without a priming 3′ OH. During DNA replication, this is supplied in the context of a short RNA primer molecule synthesized by DNA primase. The primase of archaea and eukaryotes, despite having varying subunit compositions, share...

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Detalles Bibliográficos
Autores principales: Yan, Jiangyu, Holzer, Sandro, Pellegrini, Luca, Bell, Stephen D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6042085/
https://www.ncbi.nlm.nih.gov/pubmed/29891690
http://dx.doi.org/10.1073/pnas.1806351115
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author Yan, Jiangyu
Holzer, Sandro
Pellegrini, Luca
Bell, Stephen D.
author_facet Yan, Jiangyu
Holzer, Sandro
Pellegrini, Luca
Bell, Stephen D.
author_sort Yan, Jiangyu
collection PubMed
description The cellular replicative DNA polymerases cannot initiate DNA synthesis without a priming 3′ OH. During DNA replication, this is supplied in the context of a short RNA primer molecule synthesized by DNA primase. The primase of archaea and eukaryotes, despite having varying subunit compositions, share sequence and structural homology. Intriguingly, archaeal primase has been demonstrated to possess the ability to synthesize DNA de novo, a property shared with the eukaryotic PrimPol enzymes. The dual RNA and DNA synthetic capabilities of the archaeal DNA primase have led to the proposal that there may be a sequential hand-off between these synthetic modes of primase. In the current work, we dissect the functional interplay between DNA and RNA synthetic modes of primase. In addition, we determine the key determinants that govern primer length definition by the archaeal primase. Our results indicate a primer measuring system that functions akin to a caliper.
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spelling pubmed-60420852018-07-13 An archaeal primase functions as a nanoscale caliper to define primer length Yan, Jiangyu Holzer, Sandro Pellegrini, Luca Bell, Stephen D. Proc Natl Acad Sci U S A Biological Sciences The cellular replicative DNA polymerases cannot initiate DNA synthesis without a priming 3′ OH. During DNA replication, this is supplied in the context of a short RNA primer molecule synthesized by DNA primase. The primase of archaea and eukaryotes, despite having varying subunit compositions, share sequence and structural homology. Intriguingly, archaeal primase has been demonstrated to possess the ability to synthesize DNA de novo, a property shared with the eukaryotic PrimPol enzymes. The dual RNA and DNA synthetic capabilities of the archaeal DNA primase have led to the proposal that there may be a sequential hand-off between these synthetic modes of primase. In the current work, we dissect the functional interplay between DNA and RNA synthetic modes of primase. In addition, we determine the key determinants that govern primer length definition by the archaeal primase. Our results indicate a primer measuring system that functions akin to a caliper. National Academy of Sciences 2018-06-26 2018-06-11 /pmc/articles/PMC6042085/ /pubmed/29891690 http://dx.doi.org/10.1073/pnas.1806351115 Text en Copyright © 2018 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/ This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Biological Sciences
Yan, Jiangyu
Holzer, Sandro
Pellegrini, Luca
Bell, Stephen D.
An archaeal primase functions as a nanoscale caliper to define primer length
title An archaeal primase functions as a nanoscale caliper to define primer length
title_full An archaeal primase functions as a nanoscale caliper to define primer length
title_fullStr An archaeal primase functions as a nanoscale caliper to define primer length
title_full_unstemmed An archaeal primase functions as a nanoscale caliper to define primer length
title_short An archaeal primase functions as a nanoscale caliper to define primer length
title_sort archaeal primase functions as a nanoscale caliper to define primer length
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6042085/
https://www.ncbi.nlm.nih.gov/pubmed/29891690
http://dx.doi.org/10.1073/pnas.1806351115
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