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An archaeal primase functions as a nanoscale caliper to define primer length
The cellular replicative DNA polymerases cannot initiate DNA synthesis without a priming 3′ OH. During DNA replication, this is supplied in the context of a short RNA primer molecule synthesized by DNA primase. The primase of archaea and eukaryotes, despite having varying subunit compositions, share...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6042085/ https://www.ncbi.nlm.nih.gov/pubmed/29891690 http://dx.doi.org/10.1073/pnas.1806351115 |
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author | Yan, Jiangyu Holzer, Sandro Pellegrini, Luca Bell, Stephen D. |
author_facet | Yan, Jiangyu Holzer, Sandro Pellegrini, Luca Bell, Stephen D. |
author_sort | Yan, Jiangyu |
collection | PubMed |
description | The cellular replicative DNA polymerases cannot initiate DNA synthesis without a priming 3′ OH. During DNA replication, this is supplied in the context of a short RNA primer molecule synthesized by DNA primase. The primase of archaea and eukaryotes, despite having varying subunit compositions, share sequence and structural homology. Intriguingly, archaeal primase has been demonstrated to possess the ability to synthesize DNA de novo, a property shared with the eukaryotic PrimPol enzymes. The dual RNA and DNA synthetic capabilities of the archaeal DNA primase have led to the proposal that there may be a sequential hand-off between these synthetic modes of primase. In the current work, we dissect the functional interplay between DNA and RNA synthetic modes of primase. In addition, we determine the key determinants that govern primer length definition by the archaeal primase. Our results indicate a primer measuring system that functions akin to a caliper. |
format | Online Article Text |
id | pubmed-6042085 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-60420852018-07-13 An archaeal primase functions as a nanoscale caliper to define primer length Yan, Jiangyu Holzer, Sandro Pellegrini, Luca Bell, Stephen D. Proc Natl Acad Sci U S A Biological Sciences The cellular replicative DNA polymerases cannot initiate DNA synthesis without a priming 3′ OH. During DNA replication, this is supplied in the context of a short RNA primer molecule synthesized by DNA primase. The primase of archaea and eukaryotes, despite having varying subunit compositions, share sequence and structural homology. Intriguingly, archaeal primase has been demonstrated to possess the ability to synthesize DNA de novo, a property shared with the eukaryotic PrimPol enzymes. The dual RNA and DNA synthetic capabilities of the archaeal DNA primase have led to the proposal that there may be a sequential hand-off between these synthetic modes of primase. In the current work, we dissect the functional interplay between DNA and RNA synthetic modes of primase. In addition, we determine the key determinants that govern primer length definition by the archaeal primase. Our results indicate a primer measuring system that functions akin to a caliper. National Academy of Sciences 2018-06-26 2018-06-11 /pmc/articles/PMC6042085/ /pubmed/29891690 http://dx.doi.org/10.1073/pnas.1806351115 Text en Copyright © 2018 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/ This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Biological Sciences Yan, Jiangyu Holzer, Sandro Pellegrini, Luca Bell, Stephen D. An archaeal primase functions as a nanoscale caliper to define primer length |
title | An archaeal primase functions as a nanoscale caliper to define primer length |
title_full | An archaeal primase functions as a nanoscale caliper to define primer length |
title_fullStr | An archaeal primase functions as a nanoscale caliper to define primer length |
title_full_unstemmed | An archaeal primase functions as a nanoscale caliper to define primer length |
title_short | An archaeal primase functions as a nanoscale caliper to define primer length |
title_sort | archaeal primase functions as a nanoscale caliper to define primer length |
topic | Biological Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6042085/ https://www.ncbi.nlm.nih.gov/pubmed/29891690 http://dx.doi.org/10.1073/pnas.1806351115 |
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