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Modified magnetic nanoparticles by PEG-400-immobilized Ag nanoparticles (Fe(3)O(4)@PEG–Ag) as a core/shell nanocomposite and evaluation of its antimicrobial activity

BACKGROUND: Noble metal nanoparticles, due to their good physicochemical properties, have been exploited in biological applications. Among these metals, nanosilver has attracted great attention because of its optical properties and broad-spectrum antimicrobial activities with no drug tolerance. PURP...

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Autores principales: Zomorodian, Kamiar, Veisi, Hamed, Mousavi, Seyed Mahmoud, Ataabadi, Mahmoud Sadeghi, Yazdanpanah, Somayeh, Bagheri, Jafar, Mehr, Ali Parvizi, Hemmati, Saba, Veisi, Hojat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6042532/
https://www.ncbi.nlm.nih.gov/pubmed/30022820
http://dx.doi.org/10.2147/IJN.S161002
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author Zomorodian, Kamiar
Veisi, Hamed
Mousavi, Seyed Mahmoud
Ataabadi, Mahmoud Sadeghi
Yazdanpanah, Somayeh
Bagheri, Jafar
Mehr, Ali Parvizi
Hemmati, Saba
Veisi, Hojat
author_facet Zomorodian, Kamiar
Veisi, Hamed
Mousavi, Seyed Mahmoud
Ataabadi, Mahmoud Sadeghi
Yazdanpanah, Somayeh
Bagheri, Jafar
Mehr, Ali Parvizi
Hemmati, Saba
Veisi, Hojat
author_sort Zomorodian, Kamiar
collection PubMed
description BACKGROUND: Noble metal nanoparticles, due to their good physicochemical properties, have been exploited in biological applications. Among these metals, nanosilver has attracted great attention because of its optical properties and broad-spectrum antimicrobial activities with no drug tolerance. PURPOSE: The present study has attempted to conduct chemical synthesis of Fe(3)O(4)@PEG-Ag core/shell nanocomposites in aqueous solutions through co-precipitation of Fe(3+) and Fe(2+) ions, encapsulating the iron oxide core by poly (ethylene-glycol) (PEG) improve its hydrophilicity and biocompatibility, and immobilizing silver ions by application of NaBH(4) as a reducing agent. PATIENTS AND METHODS: The synthesized structures were characterized by Fourier-transform infrared (FT-IR), field emission scanning electron microscopy, energy-dispersive X-ray spectrum, wavelength-dispersive X-ray, vibrating sample magnetometer, inductively coupled plasma-mass spectrometry and transmission electron microscopy methods. Antimicrobial activity of the nanostructures against Staphylococcus aureus, Escherichia coli and Candida albicans was evaluated by broth microdilution based on the methods suggested by Clinical Laboratory Standard Institute. Furthermore, the nanocomposite was tested for possible anti-parasitic effects against Leishmania major promastigotes by MTT assay. Also, its impacts on bacterial cell morphology were defined using atomic force microscopy. Moreover, toxicity of the nanostructure related to animal cell line was determined based on MTT assay. RESULTS: In general, the synthesized core/shell nanostructure can demonstrate noticeable activity against the evaluated representative microorganisms while its toxicity against animal cell line is not considerable. CONCLUSION: This nanostructure can be applied as a smart drug delivery system with the help of an external magnetic field or it can be used as a powerful antibiotic agent along with other antibiotics that can form a shell on its structure.
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spelling pubmed-60425322018-07-18 Modified magnetic nanoparticles by PEG-400-immobilized Ag nanoparticles (Fe(3)O(4)@PEG–Ag) as a core/shell nanocomposite and evaluation of its antimicrobial activity Zomorodian, Kamiar Veisi, Hamed Mousavi, Seyed Mahmoud Ataabadi, Mahmoud Sadeghi Yazdanpanah, Somayeh Bagheri, Jafar Mehr, Ali Parvizi Hemmati, Saba Veisi, Hojat Int J Nanomedicine Original Research BACKGROUND: Noble metal nanoparticles, due to their good physicochemical properties, have been exploited in biological applications. Among these metals, nanosilver has attracted great attention because of its optical properties and broad-spectrum antimicrobial activities with no drug tolerance. PURPOSE: The present study has attempted to conduct chemical synthesis of Fe(3)O(4)@PEG-Ag core/shell nanocomposites in aqueous solutions through co-precipitation of Fe(3+) and Fe(2+) ions, encapsulating the iron oxide core by poly (ethylene-glycol) (PEG) improve its hydrophilicity and biocompatibility, and immobilizing silver ions by application of NaBH(4) as a reducing agent. PATIENTS AND METHODS: The synthesized structures were characterized by Fourier-transform infrared (FT-IR), field emission scanning electron microscopy, energy-dispersive X-ray spectrum, wavelength-dispersive X-ray, vibrating sample magnetometer, inductively coupled plasma-mass spectrometry and transmission electron microscopy methods. Antimicrobial activity of the nanostructures against Staphylococcus aureus, Escherichia coli and Candida albicans was evaluated by broth microdilution based on the methods suggested by Clinical Laboratory Standard Institute. Furthermore, the nanocomposite was tested for possible anti-parasitic effects against Leishmania major promastigotes by MTT assay. Also, its impacts on bacterial cell morphology were defined using atomic force microscopy. Moreover, toxicity of the nanostructure related to animal cell line was determined based on MTT assay. RESULTS: In general, the synthesized core/shell nanostructure can demonstrate noticeable activity against the evaluated representative microorganisms while its toxicity against animal cell line is not considerable. CONCLUSION: This nanostructure can be applied as a smart drug delivery system with the help of an external magnetic field or it can be used as a powerful antibiotic agent along with other antibiotics that can form a shell on its structure. Dove Medical Press 2018-07-09 /pmc/articles/PMC6042532/ /pubmed/30022820 http://dx.doi.org/10.2147/IJN.S161002 Text en © 2018 Zomorodian et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Zomorodian, Kamiar
Veisi, Hamed
Mousavi, Seyed Mahmoud
Ataabadi, Mahmoud Sadeghi
Yazdanpanah, Somayeh
Bagheri, Jafar
Mehr, Ali Parvizi
Hemmati, Saba
Veisi, Hojat
Modified magnetic nanoparticles by PEG-400-immobilized Ag nanoparticles (Fe(3)O(4)@PEG–Ag) as a core/shell nanocomposite and evaluation of its antimicrobial activity
title Modified magnetic nanoparticles by PEG-400-immobilized Ag nanoparticles (Fe(3)O(4)@PEG–Ag) as a core/shell nanocomposite and evaluation of its antimicrobial activity
title_full Modified magnetic nanoparticles by PEG-400-immobilized Ag nanoparticles (Fe(3)O(4)@PEG–Ag) as a core/shell nanocomposite and evaluation of its antimicrobial activity
title_fullStr Modified magnetic nanoparticles by PEG-400-immobilized Ag nanoparticles (Fe(3)O(4)@PEG–Ag) as a core/shell nanocomposite and evaluation of its antimicrobial activity
title_full_unstemmed Modified magnetic nanoparticles by PEG-400-immobilized Ag nanoparticles (Fe(3)O(4)@PEG–Ag) as a core/shell nanocomposite and evaluation of its antimicrobial activity
title_short Modified magnetic nanoparticles by PEG-400-immobilized Ag nanoparticles (Fe(3)O(4)@PEG–Ag) as a core/shell nanocomposite and evaluation of its antimicrobial activity
title_sort modified magnetic nanoparticles by peg-400-immobilized ag nanoparticles (fe(3)o(4)@peg–ag) as a core/shell nanocomposite and evaluation of its antimicrobial activity
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6042532/
https://www.ncbi.nlm.nih.gov/pubmed/30022820
http://dx.doi.org/10.2147/IJN.S161002
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