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Characterized non-transient microbiota from stinkbug (Nezara viridula) midgut deactivates soybean chemical defenses
The Southern green stinkbug (N. viridula) feeds on developing soybean seeds in spite of their strong defenses against herbivory, making this pest one of the most harmful to soybean crops. To test the hypothesis that midgut bacterial community allows stinkbugs to tolerate chemical defenses of soybean...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6042706/ https://www.ncbi.nlm.nih.gov/pubmed/30001328 http://dx.doi.org/10.1371/journal.pone.0200161 |
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author | Medina, Virginia Sardoy, Pedro M. Soria, Marcelo Vay, Carlos A. Gutkind, Gabriel O. Zavala, Jorge A. |
author_facet | Medina, Virginia Sardoy, Pedro M. Soria, Marcelo Vay, Carlos A. Gutkind, Gabriel O. Zavala, Jorge A. |
author_sort | Medina, Virginia |
collection | PubMed |
description | The Southern green stinkbug (N. viridula) feeds on developing soybean seeds in spite of their strong defenses against herbivory, making this pest one of the most harmful to soybean crops. To test the hypothesis that midgut bacterial community allows stinkbugs to tolerate chemical defenses of soybean developing seeds, we identified and characterized midgut microbiota of stinkbugs collected from soybean crops, different secondary plant hosts or insects at diapause on Eucalyptus trees. Our study demonstrated that while more than 54% of N. viridula adults collected in the field had no detectable bacteria in the V1-V3 midgut ventricles, the guts of the rest of stinkbugs were colonized by non-transient microbiota (NTM) and transient microbiota not present in stinkbugs at diapause. While transient microbiota Bacillus sp., Micrococcus sp., Streptomyces sp., Staphylococcus sp. and others had low abundance, NTM microbiota was represented by Yokenella sp., Pantoea sp. and Enterococcus sp. isolates. We found some isolates that showed in vitro β-glucosidase and raffinase activities plus the ability to degrade isoflavonoids and deactivate soybean protease inhibitors. Our results suggest that the stinkbugs´ NTM microbiota may impact on nutrition, detoxification and deactivation of chemical defenses, and Enterococcus sp., Yokenella sp. and Pantoea sp. strains might help stinkbugs to feed on soybean developing seeds in spite of its chemical defenses. |
format | Online Article Text |
id | pubmed-6042706 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-60427062018-07-19 Characterized non-transient microbiota from stinkbug (Nezara viridula) midgut deactivates soybean chemical defenses Medina, Virginia Sardoy, Pedro M. Soria, Marcelo Vay, Carlos A. Gutkind, Gabriel O. Zavala, Jorge A. PLoS One Research Article The Southern green stinkbug (N. viridula) feeds on developing soybean seeds in spite of their strong defenses against herbivory, making this pest one of the most harmful to soybean crops. To test the hypothesis that midgut bacterial community allows stinkbugs to tolerate chemical defenses of soybean developing seeds, we identified and characterized midgut microbiota of stinkbugs collected from soybean crops, different secondary plant hosts or insects at diapause on Eucalyptus trees. Our study demonstrated that while more than 54% of N. viridula adults collected in the field had no detectable bacteria in the V1-V3 midgut ventricles, the guts of the rest of stinkbugs were colonized by non-transient microbiota (NTM) and transient microbiota not present in stinkbugs at diapause. While transient microbiota Bacillus sp., Micrococcus sp., Streptomyces sp., Staphylococcus sp. and others had low abundance, NTM microbiota was represented by Yokenella sp., Pantoea sp. and Enterococcus sp. isolates. We found some isolates that showed in vitro β-glucosidase and raffinase activities plus the ability to degrade isoflavonoids and deactivate soybean protease inhibitors. Our results suggest that the stinkbugs´ NTM microbiota may impact on nutrition, detoxification and deactivation of chemical defenses, and Enterococcus sp., Yokenella sp. and Pantoea sp. strains might help stinkbugs to feed on soybean developing seeds in spite of its chemical defenses. Public Library of Science 2018-07-12 /pmc/articles/PMC6042706/ /pubmed/30001328 http://dx.doi.org/10.1371/journal.pone.0200161 Text en © 2018 Medina et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Medina, Virginia Sardoy, Pedro M. Soria, Marcelo Vay, Carlos A. Gutkind, Gabriel O. Zavala, Jorge A. Characterized non-transient microbiota from stinkbug (Nezara viridula) midgut deactivates soybean chemical defenses |
title | Characterized non-transient microbiota from stinkbug (Nezara viridula) midgut deactivates soybean chemical defenses |
title_full | Characterized non-transient microbiota from stinkbug (Nezara viridula) midgut deactivates soybean chemical defenses |
title_fullStr | Characterized non-transient microbiota from stinkbug (Nezara viridula) midgut deactivates soybean chemical defenses |
title_full_unstemmed | Characterized non-transient microbiota from stinkbug (Nezara viridula) midgut deactivates soybean chemical defenses |
title_short | Characterized non-transient microbiota from stinkbug (Nezara viridula) midgut deactivates soybean chemical defenses |
title_sort | characterized non-transient microbiota from stinkbug (nezara viridula) midgut deactivates soybean chemical defenses |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6042706/ https://www.ncbi.nlm.nih.gov/pubmed/30001328 http://dx.doi.org/10.1371/journal.pone.0200161 |
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